DOI QR코드

DOI QR Code

Effect of dietary fermented spent mushroom (Hypsizygus marmoreus) substrates on laying hens

느티만가닥버섯 수확후배지 발효사료 급여가 산란계에 미치는 영향

  • Kim, Su Cheol (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Moon, Yea Hwang (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Kim, Hay Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Hong Chul (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Jong Ok (Namkyung Co., Ltd.) ;
  • Cheong, Jong-Chun (Mushroom Research Division, International Institute of Horticultural & Herhal Science, RDA) ;
  • Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김수철 (경남과학기술대학교 제약공학과) ;
  • 문여황 (경남과학기술대학교 동물생명과학과) ;
  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 김홍출 (경남과학기술대학교 제약공학과) ;
  • 김종옥 (남경농산) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Received : 2014.11.17
  • Accepted : 2014.12.30
  • Published : 2014.12.31

Abstract

This study was conducted to evaluate the feeding value of the spent mushroom (Hypsizygus marmoreus) substrates (SMS) in laying hens (Hy-Line Brown). The fresh spent mushroom (Hypsizygus marmoreus) substrates collected from the Namkyung farm were fermented with Bacillus subtilis EJ3 for 2 weeks. A total of twenty-four laying hens were fed corn-soy based experimental diets containing 0% (T0), 5% (T1), 10% (T2) and 15%(T3) fermented SMS for 12 weeks. There were no significant differences among the treatments in egg production, egg weight, egg mass, feed conversion and viability during the experimental period. Feed intake was significantly lowered in T0 (118.3 g) than T1 (121.9 g), T2 (120.3 g) and T3 (122.4 g). There were no significant differences among the treatments eggshell breaking strength, thickness and haugh unit, whereas the yolk color of T1, T2 and T3 were significantly heavy than T0. The palatability of boiled meat was significantly better in the T3 laying hens than in the T0 laying hens. In conclusion, fermented SMS can be used as resource of feed in laying hen feed at 5.0-15% level without effect on performance and egg qualify.

느티만가닥버섯 수확후배지 발효사료 급여가 산란계에 미치는 영향을 조사하기 위하여 도준농산에서 수거한 느티만가닥버섯 수확후배지를 Bacillus subtilis EJ3 배양액과 혼합하여 2주 동안 발효시킨 것을 공시사료로 사용하였다. 실험동물인 12주령된 Hy-line brown 갈색계 24수는 시판사료 급여구인 대조구(T0)와 5(T1), 10(T2), 15%(T3)의 버섯수확후배지 발효산물이 첨가된 시판사료 급여구인 시험구로 나누어 12주 동안 공시사료를 급여하였다. 12주동안 평균 산란율은 대조구에 비해 5% 버섯수확후배지 발효산물 첨가구에서 증가하였다가 버섯수확후배지 첨가량이 증가할수록 감소하였다. 사료섭취량은 대조구와 5% 버섯수확후배지 발효산물 첨가구에서는 큰 차이를 보이지 않았지만 버섯수확후배지 발효산물 첨가량이 증가할수록 약간 증가하였다. 사료요구율은 대조구와 5% 버섯수확후 배지 첨가구에서 비슷하게 나타났으며 10% 버섯수확후배지 첨가구부터는 버섯수확후배지 첨가량이 증가할수록 높게 나타났다. 산란량과 산란율은 대조구보다는 5% 버섯수확후배지 첨가구에서 높게 나타났다. 총 12주의 사양시험기간 동안 난중, 난각강도, 난각두께, 난황색 모두 5% 버섯수확후배지 발효산물을 첨가한 처리구에서는 대조구와 큰 차이가 없었지만 버섯수확후배지 발효산물의 첨가량이 증가할수록 감소하는 경향을 나타내었다. 계란의 내부 품질을 평가하는 기준인 난황색은 5% 버섯수확후배지 발효산물 첨가구에서는 대조구와 차이가 없었으나 10% 이상의 버섯수확후배지 발효산물을 첨가한 처리구에서는 난황색이 대조구에 비해 낮게 나타났다. 계란의 내부 오염 지표인 육반과 혈반에서는 처리구간의 차이는 없었으며 외부 품질 기준인 난각색도 처리구간에 차이를 나타내지 않았다. 그러나 계란의 포장과 수송과정에서 계란의 상품성에 영향을 미치는 요인인 난각중과 난각 두께는 5% 버섯수확후배지 발효산물 첨가구에서 대조구보다 높게 나타났다. 버섯수확후배지 발효산물의 급여구에서 산란계의 가스발생량은 버섯수확후배지 첨가량이 증가할수록 암모니아 가스 발생량이 감소하는 경향을 나타내었다.

Keywords

References

  1. AOAC. 1995. Official methods of analytical chemists, Washington DC, USA.
  2. Baba E, Nagaishi S, Fukuta T, Arakawa A. 1991. The role of intestinal microflora on the prevention of Salmonella colonization in chickens. Poultrt Sci 70:1902-1907. https://doi.org/10.3382/ps.0701902
  3. Bong MH, Ji SY, Park JC, Moon HK, Lee SC, Lee JH, Hong JK. 2011. Effect of feeding plum and red ginseng marc on vital reaction in broiler stress. Korean J Poult Sci 38(3):213-223. https://doi.org/10.5536/KJPS.2011.38.3.213
  4. Conway PL, Kjelleberg S. 1989. Protein-mediated adhension of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J Gen Microbial 135:1175-1186.
  5. Dunham HJ, William C, Edens FW, Casas IA, Dorbrogosz WJ. 1993. Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys. Poultry Sci 72(Suppl. 1):103.
  6. Feng P, Hunt CW, Pritchard GT, Julien WE. 1996. Effect of enzyme preparations on in situ and in vitro degration and in vivo digestive characteristics of mature cool-season grass forage in beef steer. J Ani Sci 74:1349-1357. https://doi.org/10.2527/1996.7461349x
  7. Fuller R. 1989. Probiotics in man and animals; A review. J Appl Bacteriol 66:365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  8. Haddadin M, Abdulrahim SM, Hashlamoun EAR, Robinson RK. 1996. The effect of Lactobacillus acidophilus on the production and chemical composition of hen's eggs. Poultry Sci 75:491-494. https://doi.org/10.3382/ps.0750491
  9. Heck JX, Hertz PF, Ayub MAZ. 2002. Cellualse and xylanase production by isolated amazon Bacillus strains using soybean industrial residue based solid-state cutivation. Braz J Microbiol 33:213-218. https://doi.org/10.1590/S1517-83822002000300005
  10. Jang BS, Yun HI, Park SC, Kim MK, Choi YW, Oh TK. 1999. Bacillus amyloiquefaciens DS11 phytase on phsporus concentration in broiler gastrointestinal tract. Kor J Vet Publ Hlth 23(1):45.
  11. Kang HK, Seo OS, Choi HC, Chae HS, Na JC, Yu DJ, Kang GH, Bang HT, Park SB, Kim MJ, Lee JE, Kim DW, Kim SH. 2010. Effects of feed supplementations for fermented apple pomace and cinamon on egg quality and performance in laying hens. Korean J Poult Sci 37(1):63-68. https://doi.org/10.5536/KJPS.2010.37.1.063
  12. Kim HJ, Woo JS, Kwon OS, Min BJ, Shon KS, Jo JH, Chen YJ, Kim IH. 2005. Effects of Bacillus subtilis supplementation on egg quality, blood characteristics and fecal $NH_3$-N in laying hens. Korean J Poult Sci 32(1):9-14.
  13. Kim JE, Park SK, Kim TW, Mun M, Koh JS, Jeong SK, Kook K. 2010. Effect of feeding fermentation of spent mushroom substrate (FSMS) on growth performance in broiler chicks. Korean J Vet Serv 33(4):387-392.
  14. Kim OK, Kim HK, Bae KS, Yu JH, Oh DK. 1997. purification of properties of a thermostable phytase from Bacillus sp. DS11. Enzyme Microb Technol 22:2.
  15. Kim YI, Jeon SH, Seok JS, Yang SY, Huh JW, Kwak WS. 2008a. Isolation and identification of hydrolytic enzymeproducing bacteria from spent mushroom substrate. J Anim Sci Technol (Kor) 50(5):713-720. https://doi.org/10.5187/JAST.2008.50.5.713
  16. Kim YI, Seok JS, Kwak WS. 2008b. Effect of mixed microbes addition on chemical change and silage storage of spent mushroom substrates. J Anim Sci Technol (Kor) 50(6):831-838. https://doi.org/10.5187/JAST.2008.50.6.831
  17. Kim YI, Lee YH, Kim KH, Oh YK, Moon YH, Kwak WS. 2012. Effect of supplementing micorobially-fermented spent mushroom substrates on growth performance and carcass characteristics of Hanwoo stress (a field study). Asian-Aust J Anim Sci 25(11):1575-1581. https://doi.org/10.5713/ajas.2012.12251
  18. Kim YJ. 2013. Effect of dietary supplementation of yacon (Polymnia sonchifolia) by-products on performance and physico-chemical properties of chicken thigh meat. Korean J Poult Sci 40(1):1-9. https://doi.org/10.5536/KJPS.2013.40.1.001
  19. Kwak WS, Jung SH, Kim YI. 2008. Broiler litter supplementation improves storage and feed-nutritional value of sawdust-based spent mushroom substrates. Bioresou Technol 99:2947-2955. https://doi.org/10.1016/j.biortech.2007.06.021
  20. Lanza M, Fasone V, Galofaro V, Barbagallo D, Bella M, Pennisi P. 2004. Citrus pulp as an ingredient in ostrich diet: effects on meat quality. Meat Science 68:269-275. https://doi.org/10.1016/j.meatsci.2004.03.005
  21. Lee JH, Kim KS, Shin SO, Cho JH, Chen YJ, Kim IH. 2007. Effects of dietary pine cone meal on egg production, egg quality, serum cholesterol and cholesterol content and fatty acid composition of egg yolk in laying hens. Korean J Poult Sci 34(3):223-229. https://doi.org/10.5536/KJPS.2007.34.3.223
  22. Lee SB, Choi YH, Cho SK, Shin TS, Cho BW, Kang HS, Kim KK, Kim SK, Lee HG. 2012. Effects of dietary Flammulina velutipes mycelium on broiler chick performance, pathogenic bacterial counts in caecal contents and amount of $NH_3$ in excreta. J Anim Sci Technol(Kor) 54:341-347. https://doi.org/10.5187/JAST.2012.54.5.341
  23. Lee SB, Wang T, Choi YH, Jeong SY, Kim WS, Kim YC, Kang HS, Kim SK, Lee HG. 2014. Effects of dietary fermented Pleurotus eryngii mycelium on performance and egg quality in laying hens. J Agri Life Sci 48(3):203-216.
  24. Lopez-Contreras AM, Gabor K, Martens AA, Renckens BAM, Claassen PAM, Van Der Oost J, De Vos WM. 2004. Substrate-induced production and secretion of cellulase production by Clostridium acetobutylicum. Appl Environ Microbiol 70:5238-5243. https://doi.org/10.1128/AEM.70.9.5238-5243.2004
  25. Mawadza C, Hatti KR, Zvauya R, Mattiasson B. 2000. Purification and characterization of cellulase produced two Bacillus strains. J Biothechnol 83:177-187. https://doi.org/10.1016/S0168-1656(00)00305-9
  26. Moon YH, Shin PG, Cho SJ. 2012. Feeding Value of Spent Mushroom (Pleurotus eryngii) substrate. J Mushroom Sci Production 10(4):236-243.
  27. Na JC, Jang BG, Kim SH, Kim JH, Kim SK, Kang HS, Lee DS, Lee SJ, Cheong JC, Lee JK. 2005. Influence of feeding Flammulina velutipes media on productivity and egg quality in laying hens. Korea J Poult Sci 32:143-147.
  28. Na, JC, Kim TH, Seo OS, Yu DJ, Kim HK, Lee SJ, Kim SH, Ha JK, Kim JH. 2003. Effects of dietary supplementation of complex probiotics on performance in laying hens. Korean J Poult Sci 30:145-150.
  29. Nahashon SN, Nakaue HS, Mirosh LW. 1996. Nutrient retention and production parameters of single comb white leghorn layers fed diets with varying crude protein levels and supplemented with direct-fed microbials. Anim Feed Sci Technol 61:17-26. https://doi.org/10.1016/0377-8401(96)00956-X
  30. Panda AK, Reddy MR, Rama Rao SV, Praharaj NK. 2003. Production performance, serum/yolk cholesterol and immune competence of white Leghorn layer as influenced by dietary supplementation. J Trop Anim Health Prod 35:85-94. https://doi.org/10.1023/A:1022036023325
  31. Park CI, Kim YJ. 2012. Effect of dietary supplementation of powdered Mulberry leaves on egg production, quality and blood characteristics in laying hens. Korean J Poult Sci 39(3):215-222. https://doi.org/10.5536/KJPS.2012.39.3.215
  32. Santoso U, Ohtani S, Tanaka K, Sakaida M. 1999. Dried Bacillus subtilis culture ammonia gas release in poultry house. Asian-Aust J Anim Sci 12:806. https://doi.org/10.5713/ajas.1999.806
  33. Sukumaran RK, Singhania RR, Pandey A. 2005. Microbial cellulase-Production, applications and challenges. J Sci Ind Res 64:832-844.
  34. Uzun I. 2004. Use of spent mushroom compost in sustainable fruit production. J. Fruit and Ornam. Plant Res Special ed 12:157-165.
  35. Yousefi M, Karkoodi K. 2007. Effect of probiotic thepax and Saccharomyces cerevisiae supplementaion on performance and egg quality of laying hens. Int J Poult Sci 6:52-54. https://doi.org/10.3923/ijps.2007.52.54