Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Pathogenicity bioassay of entomopathogenic nematode, Steinernema carpocapsae GSN1 strain (Nematoda: Steinernematidae) on the shiitake fungus moth, Morophagoides moriutii (Lepidoptera: Tineidae)

표고버섯좀나방에 대한 곤충병원성 선충의 병원성 검정

  • Kim, Hyeong Hwan (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dong Hwan (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Myung Rae (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yang, Chang Yeol (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kang, Taek Jun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jeon, Sung Wook (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Song, Jin Sun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jung, Young Hak (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Park, Hae Woong (World Institute of Kimchi, An Annex of Korea Food Research Institute) ;
  • Park, Chung Gyoo (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Choo, Ho Yul (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 김형환 (국립원예특작과학원 원예특작환경과) ;
  • 김동환 (국립원예특작과학원 원예특작환경과) ;
  • 조명래 (국립원예특작과학원 원예특작환경과) ;
  • 양창열 (국립원예특작과학원 원예특작환경과) ;
  • 강택준 (국립원예특작과학원 원예특작환경과) ;
  • 전성욱 (국립원예특작과학원 원예특작환경과) ;
  • 송진선 (국립원예특작과학원 원예특작환경과) ;
  • 정영학 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 박해웅 (세계김치연구소) ;
  • 박정규 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 추호렬 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2014.10.30
  • Accepted : 2014.12.31
  • Published : 2014.12.31
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Abstract

Pathogenicity of Steinernema carpocapsae GSN1 strain was evaluated against Morophagoides moriutii larvae. In Petridish tests, insect mortality by the nematode was dose dependent, which increased with dose from 5 to 160 infective juveniles(IJs)/larva. Pathogenicity against fourth-instar larvae was higher than the rate of corresponding second- and third-instar larvae, showing 100% insect mortality with the dose of 40 IJs/$4^{th}$ instar larva and 80 IJs/$2^{nd}$ or $3^{rd}$ instar larvae. Lethal concentration values at 50% ($LC_{50}$) of S. carpocapsae GSN1 strain were 4.2 IJs/$2^{nd}$ instar larva; 8.5 IJs/$3^{rd}$ instar larva; and 2.3 IJs/$4^{th}$ instar larva, respectively. The number of nematodes established in M. moriutii larvae after infection increased in the increment of dose and insect developmental stage. The highest number of nematodes was harvested from fourth instar larvae of M. moriutii at a dose of 160 IJs per larva, showing 22.5 nematodes per insect larva. Nematode reproductive capacity was related to insect developmental stage, showing 6,335 IJs/$2^{nd}$ instar larva, 21,660 IJs/$3^{rd}$ instar larvae, and 88,700 IJs/$4^{th}$ instar larvae.

표고버섯좀나방(Morophagoides moriutii)은 최근 버섯에 심각한 피해를 주는 해충으로 유충이 골목이나 종균, 자실체를 직접 가해하여 생산량을 감소시키고 상품성을 저하 시킨다. 표고버섯좀나방의 환경 친화적 방제에 이용할 수 있는 가능성을 알아보기 위하여 국내 토양에서 탐색된 토착 곤충병원성 선충 Steinernema carpocapsae GSN1 계통의 접종 농도별 병원성, 침입수, 증식수를 표고버섯좀나방의 령기에 따라 조사하였다. 선충의 표고버섯좀나방에 대한 병원성은 접종농도가 높을수록 증가하였다. 표고버섯좀나방 2령충, 3령충에서 유충 한 마리당 선충감염태 유충(3령충) 80마리 접종부터 100%의 높은 병원성을 나타내었고, 표고버섯좀나방 4령충은 한 마리당 선충 감염태 유충 40마리 접종부터 100%의 치사율을 나타내었다. 선충의 표고버섯좀나방 유충 체내로의 침입수는 접종 농도와 령기가 높을수록 선충의 침입수도 증가 하였다. 선충 감염태 유충 5마리 농도 처리에서는 2령충 1.3마리, 3령충 1.7마리, 4령충 1.8마리 였으나, 160마리 농도처리에서 2령충 12.3마리, 3령충 21.1마리, 4령충 22.5마리로 조사 되었다. 끝으로 표고버섯좀나방 유충 한 마리당 침입한 선충의 증식수는 같은 령기에서는 선충의 접종농도가 많아질수록 증식수가 증가하였다. 또한 표고버섯좀나방의 령기에 따라 선충 증식수의 차이는 많았는데, 선충 감염태 유충 160마리 처리농도에서는 각각 표고버섯좀나방 2령충이 6,335마리, 3령충이 21,660마리, 4령충이 88,700마리로 조사되었다. 따라서 토착 곤충병원성 선충 S. carpocapsae GSN1 계통은 표고버섯좀나방의 생물적 방제를 위한 방제제로 활용할 가능성이 높음을 알 수 있었다.

Keywords

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