The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Inula helenium on Inflammation and Insulin Resistance in Obesity-induced Insulin Resistance Mouse by High Fat Diet

목향(木香)이 고지방 식이에 의한 비만으로 유발된 인슐린 저항성 mouse의 염증 및 인슐린 저항성에 미치는 영향

  • Oh, Jae-Seon (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University) ;
  • Ma, Young-Hun (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University) ;
  • Choi, Seung-Bum (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University) ;
  • Kim, Jong-Ho (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University) ;
  • Kim, Kyung-Kook (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University) ;
  • Jeon, Sang-Yun (Dept. of Internal medicine, College of Korean Medicine, Dong-Shin University)
  • 오재선 (동신대학교 한의과대학 내과학교실) ;
  • 마영훈 (동신대학교 한의과대학 내과학교실) ;
  • 최승범 (동신대학교 한의과대학 내과학교실) ;
  • 김종호 (동신대학교 한의과대학 내과학교실) ;
  • 김경국 (동신대학교 한의과대학 내과학교실) ;
  • 전상윤 (동신대학교 한의과대학 내과학교실)
  • Published : 2014.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Obesity is an important cause of insulin resistance that leads to obese type 2 diabetes. Recently it has been found that obesity is associated with adipose tissue accumulation which causes systemic inflammation. In this study, we investigated effects of Inula helenium on the inflammation in high fat diet-induced insulin resistance mouse. Methods: Insulin resistance was induced in C57BL/6 male mice (19~21 g) on a 60% fat diet. Mice were divided into 3 groups (n=6) of normal, control and Inula helenium. After 12 weeks, body weight, FBS, oral glucose tolerance test (OGTT), serum level of insulin, epididymal fat pad, liver weight and the gene expression of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6, interleukin (IL)-10 and cluster of differentiation (CD) 68 were measured. Also, adipose tissue macrophage was analyzed by fluorescence activated cell sorting. Results: Inula helenium significantly reduces oral glucose tolerance levels, insulin serum level and adipose tissue macrophage. Also Inula helenium increased IL-10 gene expression and decreased CD68 gene expression. Conclusions: These results show that Inula helenium has anti-insulin resistance and anti-inflammatory effects on a high fat diet-induced insulin resistance mouse model.

Keywords

References

  1. 대한내과학회 해리슨내과학 편집위원회. HARRISON'S 내과학. 서울: MIP; 2010, p. 2744.
  2. 대한비만학회. 임상비만학. 서울: 고려의학; 2008, p. 143, 167, 263-4, 275.
  3. 대한당뇨병학회. 당뇨병학. 서울: 고려의학; 2005, p. 310.
  4. 대한내분비학회. 내분비대사학. 서울: 군자출판사; 2011, p. 639-40, 645, 647-8.
  5. 박용우. 비만치료의 최신지견. 서울: 한미의학; 2007, p. 49, 107, 242.
  6. Mohamed-Ali V, Pinkney JH, Coppack SW. Adipose tissue as an endocrine and paracrine organ. Int J Obes (Lond) 1998;22:1145-58. https://doi.org/10.1038/sj.ijo.0800770
  7. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW JR. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 2003;112:1796-808. https://doi.org/10.1172/JCI200319246
  8. 전국 한의과대학 신계내과학교실. 신계내과학. 서울: 군자출판사; 2011, p. 89, 91, 300, 303.
  9. 윤창렬, 이남구, 김선호. 黃帝內經素問 王氷注(中). 대전: 주민출판사; 2003, p. 231-2.
  10. 朱震亨. 丹溪醫集. 북경: 인민위생출판사; 1995, p. 324.
  11. 張介賓. 景岳全書 雜證謨(上). 서울: 한미의학; 2006, p. 836.
  12. 대성문화사편집부. 欽定 四庫全書 子部 五醫家類. 서울: 대성문화사; 1995, p. 527-8.
  13. 한의과대학 본초학 편찬위원회. 본초학. 서울: 영림사; 2012, p. 395.
  14. 이영준, 구세광, 강수진. A549세포에 대한 목향 추출물의 ROS 매개 세포독성. 대한예방한의학회지 2013;17(2):169-78.
  15. 김대성, 이성진, 이장천, 우원홍, 임규상, 문연자. 목향에탄올추출물의 ROS-MAPKs 경로를 통한 세포사멸 유도. 약학회지 2012;56(3):173-9.
  16. 오희라, 고성규. 木香과 車前草가 胃癌細胞의 活性, 增殖, 自己殺害能 및 세포주기관련 유전자 발현에 미치는 영향. 대한한방종양학회지 2001(1);7:1-18.
  17. 윤태숙, 성윤영, 장자영, 양원경, 지윤의, 김호경. 목향 추출물의 항비만 활성 효과. 韓藥作誌 2010;18(3):151-6.
  18. 이명수, 김정중, 오재민, 최민규, 송미진, 안용환, 등. 파골세포 분화에 목향 물 추출물의 효과. 동의생리병리학회지 2011;25(3):516-20.
  19. 이민석, 류도곤, 권강범. 설치류 대식세포에서 木香 추출물의 항염증 효과. 동의생리병리학회지 2011;25(2):275-9.
  20. Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Invest 2000;106(4):473-81. https://doi.org/10.1172/JCI10842
  21. 전국의과대학교수. 오늘의 진단 및 치료. 서울: 한우리; 2000, p. 1252.
  22. 권오준, 이승욱, 백선호, 한수련, 안영민, 안세영, 등. 길경 투여가 고지방, 고탄수화물 식이로 유발된 비만형 제2형 당뇨병 동물모델에 미치는 영향. 대한한의학회지 2013;34(1):1-14.
  23. Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, et al. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med 2002;8(7):731-7. https://doi.org/10.1038/nm724
  24. 백선호, 한수련, 권오준, 안영민, 안세영, 이병철. 神麯 투여가 비만형 제 2형 당뇨병 동물모델의 대사인지와 지방조직 염증반응에 미치는 영향. 대한한의학회지 2012;33(3):33-45.
  25. Hotamisligil GS, Arner P, Caro JF, Atkinson RL, Spiegelman BM. Increased adipose tissue expression of tumor necrosis factor-$\alpha$ in human obesity and insulin resistance. J Clin. Invest 1995;95:2409-15. https://doi.org/10.1172/JCI117936
  26. Hotamisligil GS, Peraldi P, Budavari A, Ellis R, White MF, Spiegelman BM. IRS-1 mediated inhibition of insulin receptor tyrosine kinase activity in TNF-alpha and obesity-induced insulin resistance. Science 1996;271(5249):665-8. https://doi.org/10.1126/science.271.5249.665
  27. Fantuzzi G. Adipose tissue, adipokines and inflammation. J Allergy Clin Immunol 2005;115:911-9. https://doi.org/10.1016/j.jaci.2005.02.023
  28. Yilma AN, Singh SR, Fairley SJ, Taha MA, Dennis VA. The anti-inflammatory cytokine, interleukin-10, inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV-inactivated Chlamydia trachomatis. Mediators Inflamm 2012;2012:1-10.
  29. Bogdan C, Vodovotz Y, Nathan C. Macrophage deactivation by interleukin 10. J Exp Med 1991;174(1):1549-55. https://doi.org/10.1084/jem.174.6.1549
  30. Di Gregorio GB, Yao-Borengasser A, Rasouli N, Varma V, Lu T, Miles LM, et al. Expression of CD68 and macrophage chemoattractant protein-1 genes in human adipose and muslce tissues. Diabetes 2005;54(8):2305-13. https://doi.org/10.2337/diabetes.54.8.2305