Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Screening for Anti-inflammatory Activities in Extracts from Korean Herb Medicines

국산 생약 추출물의 항염증 활성 스크리닝

  • An, Sang Mi (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Kim, Hyoung Gun (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Choi, Eun Jung (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Hwang, Hyoung Hoon (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Lee, Eunseok (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Baek, Ji Hwoon (Dermapro Skin Research Center, DERMAPRO LTD.) ;
  • Boo, Yong Chool (Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Medical Education Program for Human Resources, Kyungpook National University School of Medicine) ;
  • Koh, Jae Sook (Dermapro Skin Research Center, DERMAPRO LTD.)
  • 안상미 ((주)더마프로 피부과학연구소) ;
  • 김형건 ((주)더마프로 피부과학연구소) ;
  • 최은정 ((주)더마프로 피부과학연구소) ;
  • 황형훈 ((주)더마프로 피부과학연구소) ;
  • 이은석 ((주)더마프로 피부과학연구소) ;
  • 백지훈 ((주)더마프로 피부과학연구소) ;
  • 부용출 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소) ;
  • 고재숙 ((주)더마프로 피부과학연구소)
  • Received : 2013.10.11
  • Accepted : 2013.12.03
  • Published : 2014.03.31
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Abstract

Cosmetics are products used over long periods by the public, and their safety is very important. Contact dermatitis induced by cosmetics is the result of an inflammatory response of the skin to direct irritancy. The initial event that this inflammatory response is observed is the release of pro-inflammatory cytokines. In this study, the anti-inflammatory activities of extracts from Korean herb medicines were investigated using RAW264.7 macrophage. Among the fifty one extracts tested, the ethanol extracts from Biotae Orientalis Folium, Biotae Orientalis Folium (roasted), Cyperi Rhizoma, Nepetae Spica, Benincasae Semen, Dioscoreae Rhizoma, Dioscoreae Rhizoma (roasted), Mori Ramulus, Pini Ramulus and Alismatis Rhizoma reduced the cytotoxicity and inhibited the productions of Nitric oxide (NO) and cytokines such as interleukin (IL)-1${\beta}$, IL-6 and tumor necrosis factor (TNF)-${\alpha}$n lipopolysaccharide (LPS)-induced RAW264.7 macrophage. Additionally, they didn't induce the skin irritation when tested the human patch test. Overall, the result of this study suggests that the extracts of the ten Korean herb medicines are useful cosmetic agents for preventing the skin irritation.

화장품은 일반인이 장기간 연용하는 제품으로 인체 피부에 대한 안전성이 매우 중요하다. 화장품에 의한 접촉피부염은 자극성 물질이 피부에 접촉 후 침투하여 유발하는 염증반응으로 활성화된 면역세포에서 염증을 매개하는 다양한 인자를 분비함으로써 시작된다. 본 연구에서는 국산 생약 추출물을 이용하여 RAW264.7 대식 세포에서 염증 관련 인자에 대한 영향을 통해 항염증 활성을 스크리닝 하였다. 51종의 국산 생약 추출물 중 측백, 측백엽(초), 향부자, 형개, 동과자, 산약, 산약(초), 상지, 송절, 택사의 에탄올 추출물 10종이 lipopolysaccharide(LPS)에 의해 유도된 세포독성을 감소시킴과 동시에 염증 관련 인자인 Nitric oxide (NO), interleukin (IL)-1${\beta}$, IL-6, tumor necrosis factor (TNF)-${\alpha}$의 생성을 억제하는 것을 확인하였다. 또한, 이들 생약 추출물은 인체피부에서도 자극을 유발하지 않음을 인체 첩포시험을 통해 검증하였다. 따라서, 항염증 활성이 확인된 10종 국산 생약 추출물은 피부 자극을 예방할 수 있는 화장품 소재로서의 활용이 가능할 것으로 사료된다.

Keywords

References

  1. A. C. de Groot, Labelling cosmetics with their ingredients, BMJ (Clinical research ed.), 300(6740), 1636 (1990). https://doi.org/10.1136/bmj.300.6740.1636
  2. A. C. de Groot, E. G. Beverdam, C. T. Ayong, P. J. Coenraads, and J. P. Nater, The role of contact allergy in the spectrum of adverse effects caused by cosmetics and toiletries, Contact dermatitis, 19(3), 195 (1988). https://doi.org/10.1111/j.1600-0536.1988.tb02894.x
  3. E. Gendler, Adverse reactions to cosmetics, Cutis, 39(6), 525 (1987).
  4. S. Wahie, J. J. Lloyd, and P. M. Farr, Sunscreen ingredients and labelling: a survey of products available in the UK, Clinical and experimental dermatology, 32(4), 359 (2007). https://doi.org/10.1111/j.1365-2230.2007.02404.x
  5. E. G. Lee, B. M. Mickle-Kawar, and R. M. Gallucci, IL-6 deficiency exacerbates skin inflammation in a murine model of irritant dermatitis, Journal of immunotoxicology, 10(2), 192 (2013). https://doi.org/10.3109/1547691X.2012.707700
  6. M. Kamsteeg, P. A. Jansen, I. M. van Vlijmen- Willems, P. E. van Erp, D. Rodijk-Olthuis, P. G. van der Valk, T. Feuth, P. L. Zeeuwen, and J. Schalkwijk, Molecular diagnostics of psoriasis, atopic dermatitis, allergic contact dermatitis and irritant contact dermatitis, The British journal of dermatology, 162(3), 568 (2010). https://doi.org/10.1111/j.1365-2133.2009.09547.x
  7. J. Seneschal, E. Kubica, L. Boursault, J. Stokkermans, C. Labreze, B. Milpied, K. Ezzedine, and A. Taieb, Exogenous inflammatory acne due to combined application of cosmetic and facial rubbing, Dermatology, 224(3), 221 (2012). https://doi.org/10.1159/000338694
  8. G. Wolf, Nitric oxide and nitric oxide synthase: biology, pathology, localization, Histology and histopathology, 12(1), 251 (1997).
  9. I. Stewart, P. J. Schluter, and G. R. Shaw, Cyanobacterial lipopolysaccharides and human health - a review, Environmental health : a global access science source, 57 (2006).
  10. K. Aono, K. Isobe, K. Kiuchi, Z. H. Fan, M. Ito, A. Takeuchi, M. Miyachi, I. Nakashima, and Y. Nimura, In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines, Journal of cellular biochemistry, 65(3), 349 (1997). https://doi.org/10.1002/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S
  11. J. C. Park, The integrated dictionary of herb medicine, oriental medicine and functional food, ed. J. C. Park, Purunhangbok, Seoul (2011).
  12. Y. M. Park, S. J. Kim, K. H. Jo, E. J. Yang, and S. T. Jung, Anticariogenic and Antioxidant Activities from Medicinal Herbs, Journal of the Korean Society of Food Science and Nutrition, 35(3), 10 (2006).
  13. S. E. Lee, N. S. Seong, J. K. Bang, C. G. Park, J. S. Sung, and J. Song, Antioxidative Activities of Korean Medicinal Plants, Korean Journal of Medicinal Crop Science, 11(2), 8 (2003).
  14. S. Y. Park and J. W. Kim, Screening and Isolation of the Antitumor Agents from Medicinal Plants (I), Korean Journal of Pharmacognosy, 23(4), 4 (1992).
  15. G. Y. Kim, K. W. Nam, S. J. Jang, J. E. Kim, J. S. Iim, B. S. Ahn, D. S. Kwun, H. W. Jung, and K. O. Cho, Antibacterial Activities of Medicinal Herbs on Salmonella and E. Coli, Korean Journal of Oriental Physiology and Pathology, 22(3), 5 (2008).
  16. T. Mosmann, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65(1-2), 55 (1983). https://doi.org/10.1016/0022-1759(83)90303-4
  17. World Medical Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects, JAMA : the journal of the American Medical Association, (2013).
  18. Korea Food & Drug Administration, Clinical test and in vitro test guideline for cosmetics (2011).
  19. P. J. Frosch and A. M. Kligman, The soap chamber test, A new method for assessing the irritancy of soaps, J. Am. Acad. Dermatol., 1(1), 35 (1979). https://doi.org/10.1097/00000372-197901010-00004
  20. L. J. Loretz and J. E. Bailey, CTFA Safety Evaluation Guidelines, The Cosmetic, Toiletry, and Fragrance Association, (2007).
  21. Y. Yamamoto, P. He, T. Klein, and H. Friedman, Endotoxin induced cytotoxicity of macrophages is due to apoptosis caused by nitric oxide production, Journal of Endotoxin Research, 1(3), 181 (1994). https://doi.org/10.1177/096805199400100307
  22. J. L. Adams and C. J. Czuprynski, Mycobacterial cell wall components induce the production of TNF-$\alpha$, IL-1, and IL-6 by bovine monocytes and the murine macrophage cell line RAW 264.7, Microbial pathogenesis, 16(6), 401 (1994). https://doi.org/10.1006/mpat.1994.1040
  23. M. Iwamoto, M. Kurachi, T. Nakashima, D. Kim, K. Yamaguchi, T. Oda, Y. Iwamoto, and T. Muramatsu, Structure-activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264. 7 cells, FEBS letters, 579(20), 4423 (2005). https://doi.org/10.1016/j.febslet.2005.07.007
  24. D. K. Ahn, Illustrated guide to Korean medical herbs, Kyohaksa, Seoul (2003).
  25. J. Y. Kim, H. J. Kim, S. M. Kim, K. R. Park, H. J. Jang, E. H. Lee, S. H. Jung, and K. S. Ahn, Methylene chloride fraction of the leaves of Thuja orientalis inhibits in vitro inflammatory biomarkers by blocking NF-kappaB and p38 MAPK signaling and protects mice from lethal endotoxemia, Journal of ethnopharmacology, 133(2), 687 (2011). https://doi.org/10.1016/j.jep.2010.10.051
  26. H. W. Jung, S. Y. Kang, K. H. Park, T. W. Oh, J. K. Jung, S. H. Kim, D. J. Choi, and Y. K. Park, Effect of the semen extract of Thuja orientalis on inflammatory responses in transient focal cerebral ischemia rat model and LPS-stimulated BV-2 microglia, The American journal of Chinese medicine, 41(1), 99 (2013). https://doi.org/10.1142/S0192415X13500080
  27. T. H. Kim, H. Li, Q. Wu, H. J. Lee, and J. H. Ryu, A new labdane diterpenoid with anti-inflammatory activity from Thuja orientalis, Journal of ethnopharmacology, 146(3), 760 (2013). https://doi.org/10.1016/j.jep.2013.02.001
  28. Y. J. Lee, S. M. Hwang, J. J. Yoon, S. M. Lee, E. H. Kyung, J. S. Kim, D. G. Kang, and H. S. Lee, Inhibitory effect of Thuja orientalis on TNF-alpha-induced vascular inflammation, Phytother Res., 24(10), 1489 (2010). https://doi.org/10.1002/ptr.3131
  29. Y. J. Kim, H. C. Chung, H. T. Chun, K. Y. Choi, Y. G. Yun, and S. I. Jang, Hair Growth Promoting Effect of Thuja orientalis Ethanol Extracts on Hair Loss-induced DBA1J Mice, Korean J. Oriental Physiology & Pathology, 18(5), 5 (2004).
  30. H. Y. Ahn, S. J. Heo, M. J. Kang, J. H. Lee, J. Y. Cha, and Y. S. Cho, Antioxidative Activity and Chemical Characteristics of Leaf and Fruit Extracts from Thuja orientalis, Journal of Life Science, 21(5), 7 (2011).
  31. D. S. Park and J. S. Lee, Cytotoxicity against Human Normal Epithelial Cell (Beas-2B) and Antioxidant Activity of Leaves Extracts of Thuja orientalis L., J. Kor. Soc. Cosm., 16(3), 5 (2010).
  32. T. H. Youm and H. B. Lim, Antimicrobial Activities of Organic Extracts from Fruit of Thuja orientalis L., Korean J. Medicinal Crop Sci., 18(5), 315 (2010).
  33. Y. S. Lee, O. K. Han, S. W. Shin, J. H. Park, and Y. K. Kwon, Effects of Water-Extracted Cyperus Rotundus on the Nitric Oxide Production and Cytokine gene Expression, Korean J. Oriental Physiology & Pathology, 17(3), 6 (2003).
  34. K. Hemanth Kumar, A. Tamatam, A. Pal, and F. Khanum, Neuroprotective effects of Cyperus rotundus on SIN-1 induced nitric oxide generation and protein nitration: ameliorative effect against apoptosis mediated neuronal cell damage, Neurotoxicology, 34, 150 (2013). https://doi.org/10.1016/j.neuro.2012.11.002
  35. S. Kim, H. Song, K. Park, and M. Shin, Studies on the Expectorant Activities of Water Extract of Semen Benincasae and its Fractions in the Rat Trachea, The Korea Journal of Herbology, 17(1), 12 (2003).
  36. H. R. Choi, K. H. Lee, and C. H. Kim, Radiosensitizing and Antitumor Effect of the Seed of Benincasae hispida, Korean journal of food science and technology, 35(3), 4 (2003).
  37. M. Bimakr, R. A. Rahman, F. S. Taip, N. M. Adzahan, M. Z. Sarker, and A. Ganjloo, Optimization of ultrasound-assisted extraction of crude oil from winter melon (Benincasa hispida) seed using response surface methodology and evaluation of its antioxidant activity, total phenolic content and fatty acid composition, Molecules, 17(10), 11748 (2012). https://doi.org/10.3390/molecules171011748
  38. G. Y. Choi and B. W. Kim, Experimental Study on the Antioxidant and Antimicrobial Properties of Dioscoreae Rhizoma, Korean J. Orient. Int. Med., 31(2), 8 (2010).
  39. Y. J. Jun, M. Y. Son, M. J. Khil, J. S. Sung, J. C. Jeong, and D. I. Kim, Cell differentiation and Anti-oxydative effect of Dioscoreae Rhizoma on HeLa Cell, The Journal of Oriental Obstetrics & Gynecology, 20(2), 5 (2007).
  40. J. Y. Nam, J. J. Roh, J. H. Seung, M. Y. Son, M. J. Khil, J. S. Sung, and D. I. Kim, Non-toxic and Anti-oxydative effect of Dioscoreae Rhizoma on PC12 Cell, The Journal of Oriental Obstetrics & Gynecology, 19(4), 16 (2006).
  41. S. Kim, S. Shin, B. Hyun, H. Kong, S. Han, A. Lee, S. Lee, and K. Kim, Immunomodulatory Effects of Dioscoreae Rhizome Against Inflammation through Suppressed Production of Cytokines Via Inhibition of the NF-kappaB Pathway, Immune Netw., 12(5), 181 (2012). https://doi.org/10.4110/in.2012.12.5.181
  42. S. W. Shin, Y. S. Lee, J. H. Park, T. K. Kwon, S. I. Suh, and Y. K. Kwon, Comparison of Immunomodualtory Effects of Water-extracted Ginseng Radix, Pilose Asia-bell, Astragali Radix, Astractylodes Rhizoma alba and Dioscoreae Rhizoma, Korean Journal of Oriental Physiology and Pathology, 18(4), 7 (2004).
  43. S. W. Lim, S. H. Lee, J. M. Hur, Y. M. Lee, and D. K. Kim, The Inhibitory Effect of Fermented Dioscoreae batatas Extract on Lipopolysaccharide-induced Macrophage Activation, Yakhak Hoeji, 55(5), 7 (2011).
  44. J. H. Choi, H. S. Kim, M. J. Jung, and J. S. Choi, (+)-Catechin, an Antioxidant Principle from the Leaves of Pinus densiflora that Acts on 1,1- Diphenyl-2-picrylhydrazyl Radical, Natural Product Sciences, 7(1), 4 (2001).
  45. K. P. Lee and J. Y. Moon, Pinus Densiflora Gnarl Extract for Pharmacopuncture Inhibits Inflammatory Responses through Heme Oxygenase-1 Induction in Lipopolysaccharide-Stimulated RAW264.7 Macrophages, Korean Journal of Acupuncture, 29(1), 10 (2012).
  46. K. T. Lee, K. S. Lee, J. H. Jeong, B. K. Jo, M. Y. Heo, and H. P. Kim, Inhibitory effects of Ramulus mori extracts on melanogenesis, Journal of cosmetic science, 54(2), 133 (2003).
  47. K. I. Seo, Y. S. Cho, J. R. Park, S. T. Yee, and C. K. Park, Antimicrobial and Antioxidative Effects of Alismatis Rhizoma Extract, Journal of Life Science, 10(5), 5 (2000).
  48. J. W. Nam, S. H. Rhee, M. S. Kang, Y. K. Choi, C. Y. Jun, C. H. Park, and D. W. Kim, Effects of Different Lengths of Treatment with Rhizoma Alismatis on Diabetic Mellitus of Streptozotocin- Induced Hyperglycemic Rats, The Journal of Korean Oriental Internal Medicine, 27(4), 7 (2006).
  49. C. A. Toh, Antimicrobial and Antifungal Studies on Alismae Rhizoma, Korean Journal of Pharmacognosy, 27(4), 5 (1996).
  50. B. T. Ahn, S. A. Shin, J. G. Kim, and W. H. Park, Inhibitory Effect of Amentoflavone of Selaginella Tamariscina on MMP-9 Expression through NF-B and AP-1 in Macrophage Raw 264.7 cells, Korean Journal of Oriental Physiology and Pathology, 21(1), 7 (2007).
  51. D. H. Shon, D. W. Choi, and M. H. Kim, Improvement of Anti-Inflammation Activity of Gardeniae fructus Extract by the Treatment of $\beta$ -Glucosidase, Korean journal of food science and technology, 44(3), 6 (2012).
  52. B. S. Chae and T. Y. Shin, Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines, Journal of the Pharmaceutical Society of Korea, 50(3), 7 (2006).
  53. D. H. Kim, E. Y. Hwang, and J. H. Son, Anti-Inflammatory Activity of Carthamus tinctorious Seed Extracts in Raw 264.7 cell, Journal of Life Science, 23(1), 8 (2013). https://doi.org/10.5352/JLS.2013.23.1.8
  54. C. H. Kang, J. R. Koo, and J. S. So, Inhibitory Effects of Aralia cordata Thunb Extracts on Nitric Oxide Synthesis in RAW 264.7 Macrophage Cells, Korean journal of food science and technology, 44(5), 7 (2012).
  55. T. D. Kim, J. Y. Lee, B. J. Cho, T. W. Park, and C. J. Kim, The analgesic and anti-inflammatory effects of 7-oxosandaracopimaric acid isolated from the roots of Aralia cordata, Archives of pharmacal research, 33(4), 509 (2010). https://doi.org/10.1007/s12272-010-0403-2
  56. S. Y. Han and E. Yy. Lee, Effect of Paeoniae Radix Rubra Extract on the Production of NO and Prostaglandin $E_2$ in LPS-stimulated RAW264.7 Marcrophages, The Journal of Korean Acupuncture & Moxibustion Society, 28(1), 8 (2011).
  57. S. Y. Choi, K. C. Lee, Y. J. Jeoung, and B. O. Lim, In vitro Anti-inflammatory Activity of Rubus coreanus Miq. on Nitric Oxide, Interferon-$\gamma$, Cycloxygenase-2, and Tumor Necrosis Factor-$\alpha$ Production in the Macrophage like Cell Line RAW 264.7 Activated by Lipopolysccharide, Korean Journal of Medicinal Crop Science, 15(5), 5 (2007).
  58. H. W. Jung, C. H. Yoon, K. M. Park, H. S. Han, and Y. K. Park, Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-kappaB pathway, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 47(6), 1190 (2009). https://doi.org/10.1016/j.fct.2009.02.012
  59. S. Y. Lee, H. J. Park, D. S. Cha, T. Y. Shin, H. J. Na, W. S. Moon, Y. G. Kang, and H. Jeon, Antioxidant and Anti-inflammatory Effect of Angelica Tenuissima in IFN-$\gamma$/LPS-stimulated Peritoneal Macrophage, Korean J. Oriental Physiology & Pathology, 22(6), 8 (2008).
  60. S. W. Choi, E. O. Kim, H.-H. Leem, and J.-K. Kim, Anti-Inflammatory Effects of Volatile Flavor Extracts from Cnidium officinale and Angelica giga, J. Korean Soc. Food Sci. Nutr., 41(8), 9 (2012).
  61. M. Ryu, E. H. Kim, M. Chun, S. Kang, B. Shim, Y. B. Yu, G. Jeong, and J. S. Lee, Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk, Journal of ethnopharmacology, 115(2), 184 (2008). https://doi.org/10.1016/j.jep.2007.09.027

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