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Effect of Co-Culture with Various Somatic Cells during In Vitro Maturation of Immature Oocytes

미성숙 난자의 체외 성숙 시 다양한 체세포의 공동 배양 효과

  • Yoon, Junchul David (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Eun-Hye (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Hwang, Seon-Ung (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Cai, Lian (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University)
  • 윤준철 (충북대학교 수의과대학 수의학과) ;
  • 김은혜 (충북대학교 수의과대학 수의학과) ;
  • 황선웅 (충북대학교 수의과대학 수의학과) ;
  • 채련 (충북대학교 수의과대학 수의학과) ;
  • 현상환 (충북대학교 수의과대학 수의학과)
  • Received : 2014.03.17
  • Accepted : 2014.03.24
  • Published : 2014.03.31

Abstract

Recent 2 decades, including in vitro maturation (IVM), assisted reproductive technologies (ARTs) achieved noteworthy development. However the efficiency of ARTs with in vitro matured oocytes is still lower than that with in vivo oocytes. To overcome those limitations, many researchers attempted to adapt co-culture system during IVM and consequently maturation efficiency has been increased. The beneficial effects of applying co-culture system is contemplated base on communication and interaction between various somatic cells and oocytes, achievement of paracrine factors, and spatial effects of extracellular matrix (ECM) from somatic cell surface. The understanding of co-culture system can provide some information to narrow the gap between in vitro and in vivo. Here we will review current studies about issues for understanding cu-culture system with various somatic cells to improve in vitro maturation microenvironment and provide bird view and strategies for further studies.

Keywords

References

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