BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells

  • Nam, Seung Taek (Department of Life Science, College of Natural Science, Daejin University) ;
  • Hwang, Jung Hwan (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Dae Hong (Department of Life Science, College of Natural Science, Daejin University) ;
  • Park, Mi Jung (Department of Life Science, College of Natural Science, Daejin University) ;
  • Lee, Ik Hwan (Department of Life Science, College of Natural Science, Daejin University) ;
  • Nam, Hyo Jung (Department of Life Science, College of Natural Science, Daejin University) ;
  • Kang, Jin Ku (Department of Life Science, College of Natural Science, Daejin University) ;
  • Kim, Sung Kuk (Department of Life Science, College of Natural Science, Daejin University) ;
  • Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Chung, Hyo Kyun (Department of Internal Medicine, Chungnam National University) ;
  • Shong, Minho (Department of Internal Medicine, Chungnam National University) ;
  • Lee, Chul-Ho (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Ho (Department of Life Science, College of Natural Science, Daejin University)
  • Received : 2013.08.28
  • Accepted : 2013.12.19
  • Published : 2014.09.30
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Abstract

NADH:quinone oxidoreductase 1 (NQO1) is known to be involved in the regulation of energy synthesis and metabolism, and the functional studies of NQO1 have largely focused on metabolic disorders. Here, we show for the first time that compared to NQO1-WT mice, NQO1-KO mice exhibited a marked increase of permeability and spontaneous inflammation in the gut. In the DSS-induced colitis model, NQO1-KO mice showed more severe inflammatory responses than NQO1-WT mice. Interestingly, the transcript levels of claudin and occludin, the major tight junction molecules of gut epithelial cells, were significantly decreased in NQO1-KO mice. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. Taken together, these novel findings indicate that NQO1 contributes to the barrier function of gut epithelial cells by regulating the transcription of tight junction molecules.

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References

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