Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Lipolytic Effect of Supercritical Extraction from Pine Cone (Pinus koraiensis) in Mature 3T3-L1 Adipocytes

분화된 3T3-L1 세포에서 잣송이 초임계 추출물의 지방분해 효과

  • Lee, Minhee (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University) ;
  • Nam, Da-Eun (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University) ;
  • Kim, Ok Kyung (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University) ;
  • Heo, Seok Hyun (Korea Health Supplements Association) ;
  • Lee, Jeongmin (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University)
  • 이민희 (경희대학교 의학영양학과) ;
  • 남다은 (경희대학교 의학영양학과) ;
  • 김옥경 (경희대학교 의학영양학과) ;
  • 허석현 (한국건강기능식품협회) ;
  • 이정민 (경희대학교 의학영양학과)
  • Received : 2014.05.16
  • Accepted : 2014.08.06
  • Published : 2014.09.30
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Abstract

Seeds of Korean pine cone (Pinus koraiensis) have long been consumed as an edible food in countries located in North-East Asia, On the other hand, Korean pine cone, containing various polyphenols, is discarded as a useless garbage after removing seeds. This study investigated the lipolytic effects of pine cone extract in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was measured by Oil red O staining, free glycerol release by colorimetric reaction, and expression of genes related to lipid metabolism by real-time PCR. Compared to control, pine cone extract reduced intracellular lipid accumulation by 8.8% and increased free glycerol release by 8.2% a concentration of $5{\mu}g/mL$ in differentiated 3T3-L1 adipocytes. mRNA levels of fatty acid synthesis were not significantly different between control and pine cone extract, but mRNA levels of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) significantly increased by 38.7% and 94.1% at a concentration of $5{\mu}g/mL$, respectively. Thus, pine cone extract is suggested to have lipolytic effects through induction of LPL and HSL gene expression.

건강에 대한 관심의 증가로 천연식품뿐만 아니라 산업 부산물을 이용한 기능성 소재 탐색에 대한 연구들이 활발하게 진행되고 있다. 본 연구에서는 산업 부산물인 잣송이를 이용하여 초임계 방법으로 추출을 하여 항비만 식품 소재로서의 기능성을 알아보고자 하였다. 분화된 3T3-L1 지방세포에서 잣송이 초임계 추출물이 지방의 축적 및 지방분해에 미치는 영향을 확인한 결과, 잣송이 초임계 추출물을 1, $5{\mu}g/mL$ 농도로 처리 시 세포 내 지방 축적이 억제되었고 중성지방분해 산물인 glycerol의 함량은 증가하였다. 잣송이 초임계 추출물이 지방합성 효소 및 지방분해 효소에 미치는 영향을 알아보기 위해 real-time PCR을 실시한 결과, 잣송이 초임계 추출물은 지방합성 효소인 FAS에 아무런 영향이 나타나지 않았고, 지방분해 효소인 LPL과 HSL의 유전자 발현이 증가됨에 따라 세포 내 중성지방이 지방산과 glycerol로 분해되었음을 확인하였다. 따라서 이 결과들은 잣송이 초임계 추출물이 지방조직에서의 LPL과 HSL 유전자 발현 증가를 통한 지방분해로 항비만 효과를 나타냄을 보여주고 있다. 잣송이 초임계 추출물이 항비만 생리활성을 나타내는 기능성 식품 신소재로서 개발 가능성이 있을 것으로 사료된다.

Keywords

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