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Comparison of Total Polyphenols, Total Flavonoids, and Biological Activities of Black Chokeberry and Blueberry Cultivated in Korea

국내산 Black Chokeberry와 Blueberry의 총 폴리페놀, 총 플라보노이드 함량 및 생리활성 비교

  • Chung, Hai-Jung (Department of Food Science and Nutrition, Daejin University)
  • 정해정 (대진대학교 식품영양학과)
  • Received : 2014.05.13
  • Accepted : 2014.06.23
  • Published : 2014.09.30

Abstract

This study investigated the biological activities of 70% methanol extracts from black chokeberry and blueberry by measuring DPPH, superoxide anion and ABTS radical scavenging activities, metal chelating effect, reducing power, and nitrite scavenging activity. Extraction yields of black chokeberry and blueberry were 62.90% and 67.09%, respectively. Total polyphenol and total flavonoid contents were 117.20 mg/g and 32.50 mg/g for black chokeberry and 42.26 mg/g and 26.39 mg/g for blueberry, respectively. Black chokeberry had higher antioxidant activity than blueberry. DPPH and ABTS radical scavenging activities, nitrite scavenging activity, and metal chelating effect of black chokeberry were 98.29%, 96.68%, 97.64%, and 91.33%, respectively, at a concentration of 5 mg/mL, which were equal to those of ascorbic acid (positive control). These results suggest that black chokeberry has potent biological activities, and in the future, the availability of black chokeberry will increase in the field of value added food products.

본 연구에서는 국내에서 생산되는 블랙초크베리와 블루베리의 기능적 특성을 비교 조사하기 위하여 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH radical 소거능, superoxide 음이온 소거능, ABTS radical 소거능, 금속 킬레이트 효과, 환원력, 아질산염 소거능 등을 측정하였다. 총 폴리페놀 함량은 블랙초크베리가 117.20 mg/g, 블루베리가 42.26 mg/g이었으며 총 플라보노이드 함량은 블랙초크베리가 32.50 mg/g, 블루베리가 26.39 mg/g으로 나타났다. 생리활성 측정 결과 블랙초크베리는 본 실험에서 조사한 기능성에서 탁월한 활성을 나타내었는데 추출물의 농도가 증가함에 따라 전반적으로 활성이 증가하는 경향을 보였으며, 특히 DPPH radical 소거능, ABTS radical 소거능, 금속 킬레이트 효과, 아질산염 소거능은 5 mg/mL의 농도에서 90% 이상의 활성을 나타냄으로써 양성대조군으로 사용한 ascorbic acid의 활성과 대등하였다. 블루베리도 추출물의 농도증가에 따라 활성이 증가하는 경향을 나타내었으나 블랙초크베리의 0.2~0.7배에 해당하는 낮은 활성을 나타내어 블랙초크베리보다 기능성이 떨어지는 것을 알 수 있었다. 블랙초크베리와 블루베리 추출물의 총 폴리페놀 함량, 총 플라보노이드 함량과 생리활성 간의 상관관계를 조사한 결과, 총 폴리페놀 및 총 플라보노이드 농도가 증가할수록 생리활성이 증가하는 경향을 나타내었고 이들 간에는 높은 양(+)의 상관성이 있음을 보여주었다.

Keywords

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