Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparative Study on Antioxidant Effects of Extracts from Rubus coreanus and Rubus occidentalis

토종 복분자 Rubus coreanus와 외래종 복분자 Rubus occidentalis 추출물의 항산화능 비교

  • Kim, Lee Seon (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Youn, Sang Hyuck (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • 김이선 (서울과학기술대학교 식품공학과) ;
  • 윤상혁 (서울과학기술대학교 식품공학과) ;
  • 김지연 (서울과학기술대학교 식품공학과)
  • Received : 2014.05.02
  • Accepted : 2014.06.22
  • Published : 2014.09.30
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Abstract

This study compared the antioxidant effects of two kinds of black raspberry extract, obtained from fruits of Rubus coreanus and Rubus occidentalis, which can be found in Korea. The fruits of R. coreanus and R. occidentalis were each extracted with 0%, 25%, 50%, 75%, and 100% ethanol (EtOH). Among the extracts of these two varieties, 50% EtOH extract of R. occidentalis showed the highest contents of total polyphenols ($46.96{\pm}2.78mg/g$) and flavonoid compounds ($11.77{\pm}0.81mg/g$). The 50% EtOH extract of R. occidentalis showed the highest antioxidant activity ($84.77{\pm}0.97%$) in terms of DPPH radical scavenging activity. On the contrary, 25% EtOH extract of R. occidentalis showed the best antioxidant activity ($29.65{\pm}2.41%$) in terms of ABTS radical scavenging activity. In the results of ferric reducing antioxidant power (FRAP) assay, 50% EtOH extract of R. occidentalis showed the highest antioxidant activity ($0.49{\pm}0.02%$). In the cytotoxicity test stimulated with $H_2O_2$, the extracts of 75% and 100% EtOH from R. occidentalis showed the highest cell viability ($93.54{\pm}3.37%$ and $97.19{\pm}0.74%$, respectively). According to our results, extracts of R. occidentalis showed higher antioxidant activities than extracts of R. coreanus. Especially, total polyphenol and flavonoid contents of R. occidentalis showed the highest significant correlation with FRAP by Pearson's correlation (P=0.005 and P=0.013, respectively).

본 연구에서는 토종 복분자와 외래종 복분자의 항산화 활성과 장세포 산화스트레스 보호의 차이를 알아보기 위해 총 폴리페놀 및 플라보노이드 함량, DPPH 및 ABTS 라디칼소거능, FRAP assay를 이용한 환원력을 비교 조사하였고, 사람의 장내 세포인 Caco-2 cell에 과산화수소를 이용하여 자극시켜 장세포 산화스트레스 보호를 확인하였다. 총 폴리페놀 및 플라보노이드 함량은 외래종 복분자가 유의적으로 높은 것으로 나타났다. ABTS 라디칼 소거능과 FRAP assay을 이용한 환원력에서도 외래종 복분자가 유의적으로 높은 것으로 나타났으나 DPPH 라디칼 소거능은 토종과 외래종 복분자 모두 비슷하게 나타났다. 장세포 산화스트레스 보호 역시 외래종 복분자가 유의적으로 높게 나타났다. 항산화 성분과 항산화 활성의 상관관계는 모두 유의하게 양의 상관관계를 보이는 것으로 나타났으며, FRAP value가 가장 높은 상관관계를 보이는 것으로 확인되었다. 따라서 FRAP value가 높았던 외래종 복분자 100% EtOH 추출물이 폴리페놀 함량, 플라보노이드 함량, ABTS 라디칼 소거능, DPPH 라디칼 소거능, 장세포 산화스트레스 보호능 모두 높은 추출물로 확인되었다.

Keywords

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