Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Hot Water Extracts of Domestic Blue Mussel and New Zealand Green Lipped Mussel on Alcohol Metabolizing Enzymatic, DPPH Radical Scavenging, and Angiotensin Converting Enzyme Inhibitory Activities

국산 홍합과 뉴질랜드 초록입 홍합 열수 추출물의 알코올분해효소 활성에 미치는 영향 및 DPPH 라디칼 소거능과 Angiotensin Converting Enzyme 저해 활성

  • Kim, Si-Kyung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Ok, Dul-Lee (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Eunju (Department of Food and Nutrition, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
  • 김시경 (경남대학교 식품생명학과) ;
  • 옥둘이 (경남대학교 식품생명학과) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Received : 2014.05.07
  • Accepted : 2014.06.03
  • Published : 2014.09.30
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Abstract

The physiological activities of cultivated Korean blue mussel (Mytilus edulis) and New Zealand green-lipped mussel (Perna canaliculus) were analyzed and compared. Both hot water extracts of blue mussel flesh (BMF) and green-lipped mussel flesh (GMF) showed increased activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). BMF showed increased ADH and slightly decreased ALDH activities compared to GMF. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of BMF was higher than that of GMF at the same concentration. BMF and GMF showed similar inhibitory activity against angiotensin converting enzyme at a concentration of 30 mg/mL. These results suggest that cultivated Korean blue mussel has similar physiological activity with New Zealand green-lipped mussel.

우리나라 남해안 연안에서 주로 양식되는 진주담치(국산 홍합)와 세계적으로 기능성이 잘 알려진 뉴질랜드 초록입 홍합의 열수 추출물을 제조하여 생리활성을 비교하였다. 모든 홍합 추출물들은 알코올대사와 관련한 alcohol dehydrogenase(ADH)와 acetaldehyde dehydrogenase(ALDH)의 활성을 상승시켰으며, 특히 ADH의 활성을 크게 향상시켰다. 국산 홍합의 육질 추출물은 초록입 홍합의 육질 추출물에 비하여 ADH 상승 효과는 비슷하였으며, ALDH 상승 효과는 약간 낮았다. DPPH 라디칼 소거능으로 조사한 항산화능은 국산 홍합의 육질 추출물이 초록입 홍합의 육질 추출물 보다 높았다. 항고혈압과 관련한 ACE 저해능의 경우에는 국산 홍합 육질 추출물이 초록입 홍합의 육질 추출물에 비해 10 mg/mL 농도에서는 낮았으나 20 mg/mL 이상의 농도에서는 유의차(P<0.05)를 보이지 않았다. 이상의 결과는 국내에서 양식되고 있는 홍합도 세계적으로 초록입 홍합에 못지않은 생리활성을 가지고 있음을 시사한다.

Keywords

References

  1. Korean Statistical Information Service. http://kosis.kr/statisticsList/statisticsList_01List.jsp?vwcd=MT_ZTITLE&parentId=F#SubCont (accessed May 2014).
  2. Park YH. 1984. Evaluation of thermal processes for canned marine products (1) Canned boiled sea-mussel in brine and canned smoked sea-mussel in oil. Bull Korean Fish Soc 17:159-164.
  3. Lee YC, Kim DS, Kim YD, Kim YM. 1990. Preparation of oyster (Crassostrea gigas) and sea mussel (Mylitus coruscus) hydrolyzates using commercial protease. Korean J Food Sci Technol 22: 234-240.
  4. Park JS. 2011. Physicochemical properties of salt-fermented Mytilus edulis added with various seasoning sauces. Korean J Food Preserv 18: 335-340. https://doi.org/10.11002/kjfp.2011.18.3.335
  5. Taylor AG, Savage C. 2006. Fatty acid composition of New Zealand green-lipped mussels, Perna canaliculus: Implications for harvesting for n-3 extracts. Aquaculture 261: 430-439. https://doi.org/10.1016/j.aquaculture.2006.08.024
  6. McPhee S, Hodges LD, Wright PFA, Wynne PM, Kalafatis N, Harney DW, Macrides TA. 2007. Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus. Comp Biochem Physiol Part B 146: 346-356. https://doi.org/10.1016/j.cbpb.2006.11.001
  7. Treschow AP, Hodges LD, Wright PFA, Wynne PM, Kalafatis N, Macrides TA. 2007. Novel anti-inflammatory $\omega$-3 PUFAs from the New Zealand green-lipped mussel, Perna canaliculus. Comp Biochem Physiol Part B 147: 645-656. https://doi.org/10.1016/j.cbpb.2007.04.004
  8. McPhee S, Hodges LD, Wright PF, Wynne PM, Kalafatis N, Macrides TA. 2010. Prophylactic and therapeutic effects of Mytilus edulis fatty acids on adjuvant-induced arthritis in male Wistar rats. Prostaglandins Leukot Essent Fatty Acids 82: 97-103. https://doi.org/10.1016/j.plefa.2009.12.003
  9. National Fisheries Research Development Institute. 2013. http://portal.nfrdi.re.kr/page?id=aq_seafood_2_7&type=tot&from=totList&fim_col_id=2009-MF0008018-6-D01 (accessed May 2014).
  10. Charlet M, Chemysh S, Philippe H, Hetru C, Hoffmann JA, Bulet P. 1996. Innate immunity: Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusk, Mytilus edulis. J Biol Chem 271: 21808-21813. https://doi.org/10.1074/jbc.271.36.21808
  11. Kim IH, Kim JW, Lee JH. 2006. Purification of a antimicrobial peptide from the marine mussel, Mytilus coruscus. Environ Mutagen Carcin 26: 25-29.
  12. Chandran B, Rameshkumar G, Ravichandran S. 2009. Antimicrobial activity from the gill extraction of Perna viridis(Linnaeus, 1758). Global J Biotechnol Biochem 4: 88-92.
  13. Bergmeyer HU. 1974. Alcohol dehydrogenase. In Methods of Enzymatic Analysis. Bergmeyer HU, ed. Academic Press, New York, NY, USA. Vol 1, p 428-429.
  14. Koivula T, Koivusalo M. 1975. Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochim Biophys Acta 397: 9-23. https://doi.org/10.1016/0005-2744(75)90174-6
  15. Jeong SM, Kim SY, Park HR, Lee SC. 2004. Effect of farinfrared radiation on the activity of extracts from Citrus unshiu peels. J Korean Soc Food Sci Nutr 33: 1580-1583. https://doi.org/10.3746/jkfn.2004.33.9.1580
  16. Cushman DW, Cheung HS. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9
  17. SPSS. 2006. SPSS 14.0 for Windows, SPSS Inc., Chicago, IL, USA.
  18. Liber CS. 1991. Hepatic metabolic and toxic effects of ethanol. Alcohol Clin Exp Res 15: 573-592. https://doi.org/10.1111/j.1530-0277.1991.tb00563.x
  19. Cho EK, Choi YJ. 2010. Physiological activities of hot water extracts from Ecklonia cava Kjellman. J Life Sci 20:1675-1682. https://doi.org/10.5352/JLS.2010.20.11.1675
  20. Kang YM, Lee BJ, Lim JS. 2010. Alcohol metabolizing activity of fermented sea tangle juice. Kor J Fish Aquat Sci 43: 1-5.
  21. Kang BK, Jung ST, Kim SJ. 2002. Effects of vegetable extracts by solvent separation on alcohol dehydrogenase activity from Saccharomyces cerevisiae. Korean J Food Sci Technol 34: 244-248.
  22. Kim SM, Kang SH, Ma JY, Kim JH. 2006. A study on the extraction and efficacy of bioactive compound from Hovenia dulcis. Korean J Biotechnol Bioeng 21: 11-15.
  23. Park SC. 1994. Effect of bean sprout extracts on metabolism and biological functions of ethanol in vitro and in vivo. Korea Soybean Digest 10(s): 123-143.
  24. National Rural Resources Development Institute, R.D.A. 2006. 7th version Food Composition Table II. Hyoil Press, Seoul, Korea. p 134-135.
  25. National Rural Resources Development Institute, R.D.A. 2006. 7th version Food Composition Table I. Hyoil Press, Seoul, Korea. p 154-155.
  26. de Zwart FJ, Slow S, Payne RJ, Lever M, George PM, Gerrard JA, Chambers ST. 2003. Glycine betaine and glycine betaine analogues in common foods. Food Chem 83:197-204. https://doi.org/10.1016/S0308-8146(03)00063-3
  27. Craig SAS. 2004. Betaine in human nutrition. Am J Clin Nutr 80: 539-549.
  28. Brandt EG, Hellgren M, Brinck T, Bergman T, Edholm O. 2009. Molecular dynamics study of zinc binding to cysteines in a peptide mimic of the alcohol dehydrogenase structural zinc site. Phys Chem Chem Phys 11: 975-983. https://doi.org/10.1039/b815482a
  29. Wang X, Weiner H. 1995. Involvement of glutamate 268 in the active site of human liver mitochondrial (class 2) aldehyde dehydrogenase as probed by site-directed mutagenesis. Biochemistry 34: 237-243. https://doi.org/10.1021/bi00001a028
  30. National Nutrient Database for Standard Reference. USDA Agricultural Research Service. http://ndb.nal.usda.gov/ndb/search/list (accessed May 2014).
  31. Food Composition Database. Japanese Ministry of Education, Culture, Science and Technology. http://fooddb.mext.go.jp (accessed May 2014).
  32. The New Zealand Institute for Plant & Food Research Limited and Ministry of Health. The Concise New Zealand Food Composition Tables. http://www.foodcomposition.co.nz/concise-tables (accessed Jun 2014).
  33. Lee DW, You DH, Yang EK, Jang IC, Bae MS, Jeon YJ, Kim SJ, Lee SC. 2010. Antioxidant and ACE inhibitory activities of Styela clava according to harvesting time. J Korean Soc Food Sci Nutr 39: 331-336. https://doi.org/10.3746/jkfn.2010.39.3.331
  34. Fujita H, Yokoyama K, Yoshikawa M. 2000. Classification and antihypertensive activity of angiotensin I-converting enzyme inhibitory peptides derived from food proteins. J Food Sci 65: 564-569. https://doi.org/10.1111/j.1365-2621.2000.tb16049.x
  35. Corvol P, Jeunemaitre X, Charru A, Kotelevtsev Y, Soubrier F. 1995. Role of the renin-angiotensin system in blood pressure regulation and in human hypertension: new insights from molecular genetics. Recent Prog Horm Res 50: 287-308.

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