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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Antifreeze Protein from Antarctic Bacterium Flavobacterium frigoris PS1 by using Fed-batch Culture of Recombinant Pichia pastoris

재조합 Pichia pastoris의 유가식 배양을 통한 남극세균 Flavobacterium frigoris PS1 유래 결빙방지단백질의 생산

  • Kim, Eun Jae (Division of Life Sciences, Korea Polar Research Institute, KIOST) ;
  • Do, Hackwon (Division of Life Sciences, Korea Polar Research Institute, KIOST) ;
  • Lee, Jun Hyuck (Division of Life Sciences, Korea Polar Research Institute, KIOST) ;
  • Lee, Sung Gu (Division of Life Sciences, Korea Polar Research Institute, KIOST) ;
  • Kim, Hak Jun (Department of Chemistry, Pukyung National University) ;
  • Han, Se Jong (Division of Life Sciences, Korea Polar Research Institute, KIOST)
  • 김은재 (한국해양과학기술원 부설 극지연구소 극지생명과학연구부) ;
  • 도학원 (한국해양과학기술원 부설 극지연구소 극지생명과학연구부) ;
  • 이준혁 (한국해양과학기술원 부설 극지연구소 극지생명과학연구부) ;
  • 이성구 (한국해양과학기술원 부설 극지연구소 극지생명과학연구부) ;
  • 김학준 (부경대학교 화학과) ;
  • 한세종 (한국해양과학기술원 부설 극지연구소 극지생명과학연구부)
  • Received : 2014.07.04
  • Accepted : 2014.07.31
  • Published : 2014.08.31
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Abstract

Antifreeze proteins (AFP) inhibit ice growth to permit the survival of polar organisms in the cold environments. The recombinant AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced using Pichia pastoris expression system. The optimum fermentation temperature ($30^{\circ}C$) and pH (5) for FfIBP production were determined using a fed-batch culture system. The maximal cell density and purified FfIBP were 112 g/L and 70 mg/L, respectively. The thermal hysteresis (TH) activity (0.85) of FfIBP obtained using a glycerol-methanol fed-batch culture system was 2-fold higher than that of the LeIBP (Leucosporidium ice-binding protein). This work allows for large-scale production of FfIBP, which could be extended to further application studies using recombinant AFPs.

Keywords

References

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