Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Anti-inflammatory effect of Polygonum multiflorum extraction in activated RAW 264.7 cells with lipopolysaccharide

Lipopolysaccharide로 활성화된 RAW 264.7 세포에서 적하수오(Polygonum multiflorum) 추출물의 항염증 효과 검증

  • Lee, Eunsu (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kim, Hyeongjeong (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Yu, Jae-Myo (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Cho, Yong-Hun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kim, Dong-In (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Shin, Yuhyeon (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Cho, Yeongje (School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University) ;
  • Kwon, O-Jun (Regional industry Evaluation Agency for Gyeongbuk) ;
  • An, Bongjeon (Department of Cosmeceutical Science, Daegu Hanny University)
  • 이은수 (대구한의대학교 화장품약리학과) ;
  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 유재묘 (대구한의대학교 화장품약리학과) ;
  • 조용훈 (대구한의대학교 화장품약리학과) ;
  • 김동인 (대구한의대학교 화장품약리학과) ;
  • 신유현 (대구한의대학교 화장품약리학과) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 권오준 (대경지역사업평가원/경북지역산업평가단) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2014.05.21
  • Accepted : 2014.09.03
  • Published : 2014.10.30
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Abstract

The anti-inflammatory effects of Polygonum multiflorum water extracts (PMWs) and Polygonum multiflorum 70 % ethanol extracts(PMEs) were investigated using lipopolysaccharide-induce by inflammatory response. The inhibitory effects of PMWs and PMEs on the production of nitric oxide (NO) and pro - inflammatory cytokines in LPS - activated Raw 264.7 cells were investigated. The effects were examined after reducing production of Nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels. RAW 264.7 cells were cultured with LPS ($1{\mu}g/mL$) in the presence or absence of PMWs and PMEs for 24 h to determine their NO, iNOS, COX-2 levels. During the entire experimental period 10, 25, 50 and $100{\mu}g/mL$ of PMWs and PMEs showed no cytotoxicity. At these concentrations, PMWs and PMEs concentration dependently reduced the production of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-$1{\beta}$ (IL-$1{\beta}$). PMWs and PMEs were inhibited the activittion of iNOS, COX-2 by 89%, 54%, 91% and 57% respectively, at $100{\mu}g/mL$. These results indicate that PMWs and PMEs significantly reduces the effect of oxidative and inflammatory cytokines.

적하수오 추출물의 추출용매별 항염증 작용을 알아보기 위해 LPS로 자극된 RAW 264.7 세포를 이용하여 실험을 진행 한 결과 열수 추출물 및 70% 에탄올 추출물 모두 $100{\mu}g/mL$에서 60%이상의 NO 생성 억제 율을 보이는 것을 확인 하였다. 또한 사이토 카인들에 대하여 적하수오 추출물은 TNF-${\alpha}$, IL-$1{\beta}$, IL-6, $PGE_2$ 생성량은 농도 의존적으로 감소하였고, 이는 적하수오 추출물이 염증성 사이토 카인의 발현을 억제 하여 염증 발생을 억제 할 수 있다고 할 수 있다. PMW는 $100{\mu}g/mL$의 농도에서 iNOS 단백질 발현량이 89%, COX-2는 54%의 효과를 나타내었으며, PME는 $100{\mu}g/mL$의 농도에서 iNOS 91%, COX-2는 57%의 단백질 발현 저해효과가 있음을 확인 할 결과로 보아 적하수오는 대식세포에서 NO, TNF-${\alpha}$, IL-$1{\beta}$, IL-6, PGE2 iNOS, COX-2 발현을 억제 하며, 특히 적하수오 70% 에탄올 추출물의 경우 저 농도에서부터 효과를 나타내어 우수한 항염증 소재임을 확인 할 수 있었다.

Keywords

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