대한치과보철학회지 (The Journal of Korean Academy of Prosthodontics)

  • 제52권4호
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  • Pages.298-304
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  • 2014
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  • 0301-2875(pISSN)
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  • 2005-3789(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
권호 표지
DOI QR코드

DOI QR Code

rhTGF-${\beta}2$/PLGA 복합체를 electrospray법으로 코팅한 타이타늄 임플란트 골 유착의 microCT 계측: a preliminary rabbit study

Osseointegration of the titanium implant coated with rhTGF-${\beta}2$/PLGA particles by electrospray: a preliminary microCT analyzing rabbit study

  • 이우성 (서울대학교 치의학대학원 치과보철학교실) ;
  • 김성균 (서울대학교 치의학대학원 치과보철학교실) ;
  • 허성주 (서울대학교 치의학대학원 치과보철학교실) ;
  • 곽재영 (서울대학교 치의학대학원 치과보철학교실) ;
  • 이주희 (울산대학교 서울아산병원 치과보철과) ;
  • 박지만 (이화여자대학교 이대목동병원 치과보철과) ;
  • 박윤경 (서울대학교 치의학대학원 치학연구소)
  • Lee, Woo-Sung (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Kyun (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Heo, Seong-Joo (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Koak, Jai-Young (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Lee, Joo-Hee (Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Park, Ji-Man (Department of Prosthodontics, Ewha Womans University) ;
  • Park, Yoon-Kyung (Department of Dental Research Institute, Brain Korea 21, Seoul National University)
  • 투고 : 2014.08.05
  • 심사 : 2014.09.16
  • 발행 : 2014.10.31
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초록

목적: 본 선행 연구는 recombinant human transforming growth factor-${\beta}2$ (rhTGF-${\beta}2$)/ poly lactic-co-glycolic acid (PLGA) 복합체를 타이타늄 임플란트에 처리하였을 때 골 유착에 미치는 영향을 알아보기 위해 시행된 것으로 토끼 모델을 사용하였다. 재료 및 방법: 8개의 임플란트를 300V에서 3분 동안 양극 산화하였다. 그 중 4개는 electrospray법으로 rhTGF-${\beta}2$/PLGA를 코팅하여 실험군으로 설정하였다. 4마리의 New Zealand rabbit의 tibiae에 1개씩의 실험군과 대조군 임플란트를 식립하였으며, 3주와 6주에 2마리씩 희생하여 micro-computed tomography(microCT) 촬영 후 분석하였다. 결과: Scanning electron microscope (SEM) 사진에서 rhTGF-${\beta}2$/PLGA 입자가 임플란트 표면에 균일하게 분산되어 있음을 확인하였다. MicroCT 분석 결과 통계적으로 유의하지는 않지만 rhTGF-${\beta}2$/PLGA를 처리한 임플란트가 bone volume/total volume (BV/TV)와 trabecular thickness (Tb.Th) 값이 더 높은 경향성을 보였으며, cross sectional view에서 더 많은 골이 형성되었음을 확인하였다. 결론: rhTGF-${\beta}2$/PLGA 표면처리된 임플란트가 주변 골의 양적 성장을 촉진시킬 수 있으며 임플란트 초기 골 유착을 증진시킬 수 있는 가능성을 보였다.

Purpose: This preliminary rabbit study was conducted to evaluate the effect of recombinant human transforming growth factor-${\beta}2$ (rhTGF-${\beta}2$)/poly lactic-co-glycolic acid (PLGA) coating on osseointegration of the titanium (Ti) implant. Materials and methods: Eight Ti implants were anodized with 300 voltages for three minutes. Four of those were coated with rhTGF-${\beta}2$/PLGA by an electrospray method as the experimental group. The implants were placed into tibiae of four New Zealand rabbits, two implants per a tibia, one implant per each group. After 3 and 6 weeks, every two rabbits were sacrificed and micro-computed tomography (microCT) was taken for histomorphometric analysis. Results: In scanning electron microscope (SEM) image, the surface of rhTGF-${\beta}2$/PLGA coated Ti implant showed well distributed particles. Although statistically insignificant, microCT analysis showed that experimental group has higher bone volume / total volume (BV/TV) and trabecular thickness (Tb.Th) values relatively. Cross sectional view also showed more newly formed bone in the experimental group. Conclusion: In the limitation of this study, rhTGF-${\beta}2$/PLGA particles coating on the Ti implant show the possibility of more favorable quantity of newly formed bone after implant installation.

키워드

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