Membrane Journal (멤브레인)

The Membrane Society of Korea (한국막학회)
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Preparation of Phenoxy-Methylamino Cosubstituted Polyphosphazene Diagnostic Membranes for Blood Glucose Measurements

혈당 측정을 위한 페녹시-메틸아미노기가 함께 치환된 폴리포스파젠 진단막의 제조

  • Kwon, Suk-Ky (Department of Biological and Chemical Engineering, Hongik University)
  • 권석기 (홍익대학교 과학기술대학 바이오화학공학과)
  • Received : 2014.06.30
  • Accepted : 2014.08.11
  • Published : 2014.08.30
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Abstract

The water-insoluble poly(phenoxy-methylamino)phosphazene has been synthesized and investigated as the new type of diagnostic membrane for the measurements of blood glucose level. Final absorbances at 680 nm through activated polyphosphazene membranes were measured at various concentration of glucose in blood. The end-point results of varing absorbance values according to time (K/S) were used to obtain glucose concentration. The effects of substitution rates with hydrophilic methylamino groups and hydrophobic phenoxy groups on the measurements of glucose concentration were studied. Dose-response slope (DRS) values between glucose concentration and K/S values increased as the hydrophilic substitution rates increased. However, in less than 5% of the hydrophilic substitution rates, DRS values are too low, and in more than 25% of the hydrophilic substitution rates, because DRS increased rapidly, it was difficult to measure exact concentration level of glucose.

물에 녹지 않는 폴리(페녹시-메틸아미노)포스파젠을 합성하여 혈당 측정을 위한 새로운 형태의 진단막으로서의 가능성을 조사하였다. 혈액속의 글루코우즈의 농도를 변화시켜가며 활성화된 폴리포스파젠 진단막을 가지고 680 nm에서의 최종흡광도를 측정하였다. 시간에 따라 변화하는 흡광도의 값(K/S)을 이용해 글루코우즈의 농도를 얻었다. 친수성인 메틸아미노기와 소수성인 페녹시기의 치환율이 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 친수성기의 치환율이 증가함에 따라 글루코우즈의 농도와 K/S와의 기울기 값(Dose-Response Slope : DRS)이 점차적으로 증가하였다. 그러나 친수성기의 치환율이 5% 이하일 때는 DRS가 너무 낮았고 친수성기의 치환율이 25% 이상일 경우에는 DRS가 급격히 증가하여 정확한 글루코우즈의 농도측정이 어려웠다.

Keywords

References

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