Effects of Antioxidant Activities of Small Colored Potatoes (Solanum tuberosum L.) by using Ultra High Pressure Extraction Process

초고압 처리가 꼬마칼라감자의 항산화 증진에 미치는 영향

  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym Polytechnic University, Research Institute of Biomaterial, Hallym Polytechnic University) ;
  • Kwon, Min-Soo (Rokya Agricultural Corp.) ;
  • Hwang, Young-Jeong (Dept. of Food Science & Culinary Arts, International University of Korea) ;
  • Choi, Mi-Sook (Dept. of Food Technology and Services, Eulji University) ;
  • Rha, Young-Ah (Dept. of Food Technology and Services, Eulji University)
  • 박성진 (한림성심대학교 관광외식조리과/한림성심대학교 생물소재연구소) ;
  • 권민수 (농업회사법인 록야 주식회사) ;
  • 황영정 (한국국제대학교 외식조리학과) ;
  • 최미숙 (을지대학교 식품산업외식학과) ;
  • 나영아 (을지대학교 식품산업외식학과)
  • Received : 2014.04.22
  • Accepted : 2014.07.15
  • Published : 2014.08.31

Abstract

We investigated a method to improve antioxidant activities of colored potato extracts by ultra high pressure extraction process. The colored potato was extracted by water at $60^{\circ}C$(WE) and 300 MPa for 15 min (High Pressure Extraction, $HPE_{15}$) and 30 min (High Pressure Extraction, $HPE_{30}$). The extractions yielded by different extraction processes were 1.73(WE), 2.10($HPE_{15}$), and 2.41($HPE_{30}$)%. Total phenolic acid contents of different extraction processes were estimated as 48.21(WE), 50.20($HPE_{15}$) and 51.34($HPE_{30}$) GAL mg/g, respectively. The flavonoids contents of different extraction processes were measured as 13.12(WE), 14.35($HPE_{15}$) and 15.17($HPE_{30}$) RE mg/g, respectively. Generally, for the contents of phenolic acid and flavonoids, the samples from HPE were higher than those from conventional extraction process. $HPE_{30}$ showed 76.21% of DPPH radical scavenging activity (EDA, %) in 1,000 ug/mL. The reducing power of $HPE_{30}$ also showed the high activity as 0.42. In generally, antioxidant activities of colored potato were increased by high pressure extraction process. We could tell that the HPE extracts of colored potato had a higher antioxidant activity than those from conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time.

본 연구에서는 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 초고압 추출공정에 의한 꼬마칼라감자의 항산화 활성 증진을 확인하고자 연구를 수행하였다. 초고압 처리 추출물의 수율이 15분 처리시 2.10, 30분 처리시 2.41%로 추출 수율을 나타내어 일반 열수추출공정(1.73%)과 비교하여 약 1.2~1.4배의 추출수율을 나타내었다. 초고압 공정에 따른 변화 비교에서는 초고압 공정을 병행하였을 시 총 페놀과 플라보노이드 함량이 초고압 공정을 거치지 않은 것보다 다소 증가되는 것으로 보아 활성성분의 용출이 증진된 것으로 보인다. DPPH radical 소거 활성은 30분 초고압 처리한 추출물이 1.0 mg/mL에서 76.21%로 높은 활성을 나타내었으며, 환원력 역시 전체적으로 초고압 공정을 실시하였을 때의 활성이 높게 측정되었다. 초고압 추출이 꼬마칼라감자 내부 조직까지 영향을 주어 세포벽이 깨어지면서 조직 및 구조가 변화한 것으로 판단되며, 이를 통해 수율 및 활성 성분의 용출 증가가 이루어 진 것으로 사료된다. 따라서 꼬마칼라감자의 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 이룰 것으로 판단된다.

Keywords

References

  1. Ahn GJ (2010). Quality characteristics of Sulgidduk prepared with amount of purple sweet-potato powder. Korean J Culinary Res 16(1): 127-136.
  2. Ames BN (1984). Dietary carcinogen and anti-carcinogens. Clin Toxicol 22(3): 291-301. https://doi.org/10.3109/15563658408992561
  3. Bennett PB, Marquis RE, Demchenko I (1998) High pressure biology and medicine. University of Rochester Press, New York, USA, 1-428
  4. Block G, Langseth L (1994). Antioxidant vitamins and disease prevention. Food Technol 48(7):80-84.
  5. Choi, HD, Lee HC, Kim SS, Kim YS, Lom HT, Ryu GH (2008). Nutrient components and physicochemical propertiesof new domestic potato cultivars. Kor J Food Sci Technol 40(4):382-388.
  6. Fitzpatrick DF, Hirschfield SL, Coffey RG (1993). Endothelium-dependent vasorelaxing activity of wine and other grape products. Am J Physiol 265(2): H774-H778.
  7. Gutfinger T (1981). Polyphenols in olive oils. JAOCS 58(11): 966-967. https://doi.org/10.1007/BF02659771
  8. Gutteridge JMC, Halliwell B (1994). Antioxidants In nutrition, Health, and Disease. Oxford University Press. London, UK, 1-62.
  9. Horie Y, Kimura K, Ida M, Yosida Y, Ohki K (1991). Jam preparation by pressurization. Nippon Nogeikagaku Kaishi 65(6): 975-980. https://doi.org/10.1271/nogeikagaku1924.65.975
  10. Hur J (2007). Effect of Citrate and Phosphate on the Inhibition of Browning in Minimally Processed Potatoes. Korean J Culinary Res 13(2): 254-259.
  11. Jang HL, Yoon KY (2012). Biological activities and total phenolic content of ethanol extracts of white and flesh-colored Solanum tubersum L. potatoes. J Korean Soc Food Sci Nutr 41(8):1035-1040. https://doi.org/10.3746/jkfn.2012.41.8.1035
  12. Jeong JC, Chang DG, Yoon YH, Park CS, Kim SY (2006). Effect of cultural environments and nitrogen fertilization levels on the antocyanin accumulation of purple-fleshed potato. J Bio-Environ 15(2): 201-210.
  13. Kalt W (2006). Effects of production and processing factors on major fruit and vegetable antioxidant. J Food Sci 70(1): 11-19.
  14. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH, Lee HY (2007). Toxicity reduction and improvement of anticancer activities from Rhodiola sachalinensis A. Bor by ultra high pressure extracts process. Korean J Medicinal Crop Sci 15(6): 411-416.
  15. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, Park UY, Lee HY (2008). Skin-Whitening and UV protective effects of Angelica gigas Nakai extracts on uitra high pressure extraction process. Korean J Medicinal Crop Sci 16(4): 255-260.
  16. Kim HK, Kwon YJ, Kim YE, Nahmgang B (2004). Changes of total polyphenol content and antioxidant activity of Aster scaber thunb extracts with different microwave assisted extraction conditions. Korean J Food Preserv 11(1): 88-95.
  17. Kim JH, Kim JK, Kang WW, Ha YS, Choi SW, Moon KD (2003). Chemical composition and DPPH radical scavenger activity in different sections of safflower. J Korean Soc Food Sci Nutr 32(5): 733-738. https://doi.org/10.3746/jkfn.2003.32.5.733
  18. Kim TK, Shin HD, Lee YH (2003). Stabilization of polyphenolic antioxidants using inclusion complexation with cyclodextrin and their utilization as the fresh-food preservative. Korean J Food Sci Technol 35(2): 266-272.
  19. Kromhout D (1987). Essential micronutrients in relation to carcinogenesis. Am J Clin Nutr 45(5):1361-1467. https://doi.org/10.1093/ajcn/45.5.1361
  20. Lee HH, Lee SY (2008). Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean J Medicinal Crop Sci 16(2): 79-85.
  21. Lee JS (2012). Quality characteristics of wet noodles added with freeze-dried purple sweet potato powder. Korean J Culinary Res 18(5):279-292.
  22. Lee OH, Lee BY, Lee J, Lee HB, Son JY, Park CS, Shetty K, Kim YC (2009). Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities. Bioresour Technol 100(23): 6107-6113. https://doi.org/10.1016/j.biortech.2009.06.059
  23. Li H, Choi YM, Lee JS, Park JS, Yeon KS, Han CD (2007). Drying and antioxidant characteristics of the shiitake(Lentinus edodes) mushroom in a conveyer-type far-infrared dryer. J Korean Soc Food Sci Nutr 36(2): 250-254. https://doi.org/10.3746/jkfn.2007.36.2.250
  24. Ling J, Han JG, Ha JH, Jeong HS, Kwon MC, Ahn JH, Kim JC, Choi GP, Chung EK, Lee HY (2008). Effect of immune activity on Berberis koreana Palibin by ultra high pressure low temperature process. Korean J Medicinal Crop Sci 16(6): 439-445.
  25. Macrae RG, Robinson RK, Sadler MJ (1993). Encyclopedia of food science. Food Technology and Nutrition 1: 607-171.
  26. Moreno MIN, Isla MIN, Sampietro AR, Vattuone MA (2000). Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthropharmacology 71(1-2): 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  27. Oyaizu M (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44(6): 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  28. Park SJ, Seong DH, Park DS, Kim SS, Gou J, Ahn JH, Yoon YB, Lee HY (2009). Chemical compositions of fermented Codonopsis lanceolata. J Korea Soc Food Sci Nutr 38(3): 396-400. https://doi.org/10.3746/jkfn.2009.38.3.396
  29. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY (2004). Effect of ultrasonification process on enhancement of immuno-stimulatory activity of Ephedra sinica Stapf and Rubus coreanus Miq. Korean J Biotechnol Bioeng 19(2): 113-117.
  30. Park YM, Kim MH, Yoon HH (2012). Quality characteristics of Sulgidduck added with purple sweet potato. Korean J Culinary Res 18(1):54-64.
  31. Que F, Mao L, Zhu C, Xie G (2006). Antioxidant properties of Chineses yellow wine, its concentrate, and volatiles. LWT-Food Sci Technol 39(2): 111-117. https://doi.org/10.1016/j.lwt.2005.01.001
  32. Reddivari L, Hale AL, Miller JC (2007). Determination of phenolic content, composition and their contribution to antioxidant activity in specialty potato selections. Am J Potato Res 84(4):275-282. https://doi.org/10.1007/BF02986239
  33. Roberta R, Nicoletta P, Anna P, Anath P, Min Y, Catherine RE (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26(9-10): 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  34. Sato M, Ramarathanm N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H (1996). Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. J Agric Food Chem 44(1):37-41. https://doi.org/10.1021/jf950190a
  35. Song ES, Park SJ, Woo NRA, Won MH, Choi JS, Kim JG, Kang MH (2005). Antioxidant capacity of colored barley extracts by varieties. Kor J Soc Food Sci Nutr 34(10): 1491-1497. https://doi.org/10.3746/jkfn.2005.34.10.1491
  36. Velioglu YS, Mazza G, Gao L, Oomah BD (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46(10): 4113-4117. https://doi.org/10.1021/jf9801973
  37. Yamashita A, Soga Y, Iwamoto Y, Asano T, Li Y, Abiko Y, Nishimura F (2008). DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin. Int J Obesity 32(11): 1725-1729. https://doi.org/10.1038/ijo.2008.153