Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Possibility of Repeated Use of Elite Donor Cows for Mass Production of OPU-Derived Embryos

OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구

  • Jin, Jong-In (Dept. of Animal Science, Division of Applied Life Science (BK21 Plus)) ;
  • Choi, Byung-Hyun (Dept. of Animal Science, Division of Applied Life Science (BK21 Plus)) ;
  • Kim, Seong-Su (Dept. of Animal Science, Division of Applied Life Science (BK21 Plus)) ;
  • Park, Bun-Young (Gyeongnam Animal Science and Technology, Gyeongsang National University(GAST)) ;
  • Lee, Jung-Gyu (Dept. of Animal Science, Division of Applied Life Science (BK21 Plus)) ;
  • Kong, Il-Keun (Dept. of Animal Science, Division of Applied Life Science (BK21 Plus))
  • 진종인 (경상대학교 응용생명과학부(BK21 Plus) 축산학과) ;
  • 최병현 (경상대학교 응용생명과학부(BK21 Plus) 축산학과) ;
  • 김성수 (경상대학교 응용생명과학부(BK21 Plus) 축산학과) ;
  • 박번영 (경남동물과학기술) ;
  • 이정규 (경상대학교 응용생명과학부(BK21 Plus) 축산학과) ;
  • 공일근 (경상대학교 응용생명과학부(BK21 Plus) 축산학과)
  • Received : 2015.04.21
  • Accepted : 2015.05.18
  • Published : 2015.09.30
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Abstract

This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of $1^{st}$ period was carried out 22~48 sessions from each donors. And the break time for OPU-IVP of $2^{nd}$ period after $1^{st}$ OPU from each donors were 2~25 months. The OPU-IVP of $2^{nd}$ period each donors conducted total 15~65 times for 2~8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from $1^{st}$ period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for $1^{st}$ period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of $2^{nd}$ OPU-IVP was carried out after 2~25 months rest periods from $1^{st}$ period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from $1^{st}$ period OPU compared with $2^{nd}$ period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, $1^{st}$ period OPU-IVP was more efficient compared with $2^{nd}$ period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after $1^{st}$ period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.

Keywords

References

  1. Aller JF, Mucci NC, Kaiser GG, Callejas SS and Alberio RH. 2012. Effect of repeated eCG treatments and ovum pick-up on ovarian response and oocyte recovery during early pregnancy in suckling beef cows. Anim. Reprod. Sci. 133:10-5. (DOI:10.1016/j.anireprosci.2012.06.001)
  2. Andrade JC, Oliveira MA, Lima PF, Guido SI, Bartolomeu CC, Tenorio Filho F, Pina VM, Iunes-Souza TC, Pauld NR and Freitas JC. 2003. The use of steroid hormones in superovulation of Neiore donors at different stages of estrous cycle. Anim. Reprod. Sci. 77:117-25. (DOI:10.1016/S0378-4320(03)00036-8)
  3. Bols PEJ, Ysebaert MT, Van Soom A and de Kruif A. 1996. Effects of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocyte complexes. Theriogenology. 47:1221-36. (DOI:10.1016 /S0093-691X(97)00102-7)
  4. Bungartz L, Lucas-Hahn A, Rath D and Niemann H. 1995. Collection of oocytes from cattle via follicular aspiration aided by ultrasound with or without gonadotropin pretreatment and in different reproductive stages. Theriogenology. 43:667-75. (DOI:10.1016 /0093-691X(94)00072-3) https://doi.org/10.1016/0093-691X(94)00072-3
  5. Chaubal SA, Molina JA, Ohlrichs CL, Ferre LB, Faber DC, Bols PEJ, Riesen JW, Tian X and Yang X. 2006. Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows. Theriogenology. 65:1631-48. (DOI:10. 1016/j.theriogenology.2005.07.020) https://doi.org/10.1016/j.theriogenology.2005.07.020
  6. Chaubal SA, Ferre LB, Molina JA, Faber DC, Bols PEJ, Rezamand P, Tian X and Yang X. 2007. Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system. Theriogenology. 67:719-28. (DOI:10.1016/j.theriogenology.2006.07.022)
  7. De Roover R, Genicot G, Leonard S, Bols P and Dessya F. 2005. Ovum pick-up and in vitro embryo production in cows superstimulated with an individually adapted super-stimulation protocol. Anim. Reprod. Sci. 86:13-25. (DOI:10.1016/j.anireprosci.2004.05.022)
  8. De Roover R, Feugang JMN, Bols PEJ, Genicot G and Hanze Ch. 2008. Effects of ovum pick-up frequency and FSH stimulation: A retrospective study on seven years of beef cattle in vitro embryo production. Reprod. Domest. Anim. 43:239-45. (DOI: 10.1111 /j.1439-0531.2007.00873.x) https://doi.org/10.1111/j.1439-0531.2007.00873.x
  9. Deb GK, Dey SR, Bang JI, Park HC, Lee JG and Kong IK. 2011. 9-cis retinoic acid improves developmental competence and embryo quality during in vitro maturation of bovine oocytes through the inhibition of oocyte tumor necrosis factor-alpha gene exppression. J. Anim. Sci. 89:2759-67. (DOI:10.2527/jas.2011-3848)
  10. Galli C, Crotti G, Notari C, Turini P, Duchi R and Lazzari G. 2001. Embryo production by ovum pick up from live donors. Theriogenology. 55:1341-57. (DOI:10.1016/S0093- 691X(01)00486-1)
  11. Galli C, Duchi R, Colleoni S, Labutina I and Lazzari G. 2014. Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice. Theriogenology. 81: 138-51. (DOI:10.1016/j.theriogenology.2013.09.008)
  12. Garcia A and Salaheddine M. 1998. Effects of repeated ultrasound- guided transvaginal follicular aspiration on bovine oocyte recovery and subsequent follicular development. Theriogenology. 50:575-85. (DOI:10.1016/S0093-691X(98)001-629)
  13. Hasler JF, Henderson WB, Hurtgen PJ, Mc-Cauley AD, Hower SA, Shuey LS, Stokes JE and Trimmer SA. 1995. Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology. 43:141-59. (DOI:10. 1016/0093-691X(94)00020-U) https://doi.org/10.1016/0093-691X(94)00020-U
  14. Looney CR, Lindsey BR, Gonseth CL and Johnson DL. 1994. Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cow. Theriogenology. 41:67-72. (DOI:10.1016/S0093-691X(05)80050-0)
  15. Lopes AS, Martinussen, T, Greve T and Callesen H. 2006. Effect of days post-partum, breed and ovum pick-up scheme on bovine oocyte recovery and embryo development. Reprod. Domest. Anim. 41:196-203. (DOI: 10.1111/j.1439-0531.2006.00683.x)
  16. Machado SA, Reichenbach HD, Weppert M, Wolf E and Gancalves PB. 2006. The variability of ovum pick-up response and in vitro embryo production from monozygotic twin cow. Theriogenology. 65:573-83. (DOI:10.1016/j.theriogenology.2005.04.032)
  17. Mapletoft R and Hasler J. 2005. Assisted reproductive technologies in cattle: A review. Rev. Sci. Tech. Off. Int. Epiz. 24:393-403. (PMID:16110904) https://doi.org/10.20506/rst.24.1.1582
  18. Merton JS, de Roos APW, Mullaart E, de Ruigh L, Kaal L, Vos PL and Dieleman SJ. 2003. Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology. 59:651-74. (DOI:10.1016 /S0093-691X(02)01246-3) https://doi.org/10.1016/S0093-691X(02)01246-3
  19. Merton JS, Knijn HM, Flapper H, Dotinga F, Roelen BAJ, Vos PLAM and Mullarrt E. 2013. Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics. Theriogenology. 80:365-71. (DOI:10.1016/j.theriogenology.2013.04.025)
  20. Moreira F, Badinga L, Burnley C and Thatcher WW. 2002. Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactating recipient cows. Theriogenology. 57:1371-87. (DOI:10.1016 /S0093-691X(01)00719-1) https://doi.org/10.1016/S0093-691X(01)00719-1
  21. Petyim S, Bage R, Hallap T, Bergqvist A, Rodriguez-Martinez H and Larsson B. 2003. Two different schemes of twiceweekly ovum pick-up in dairy heifers: effect on oocyte recovery and ovarian function. Theriogenology. 60:175-88. (DOI:10.1016 /S0093-691X(02)01363-8) https://doi.org/10.1016/S0093-691X(02)01363-8
  22. Pieterse MC, Kappen KA, Kruip TM and Taverne MA. 1988. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology. 30:751-62. (DOI: 10.1016/0093-691X(88)90310-X)
  23. Pieterse MC, Vos PLAM, Kruip TM, Wurth YA, van Beneden TH, Willemse AH and Taverne MA. 1992. Repeated transvaginal ultrasound-guided ovum pick-up in eCG-treated cow. Theriogenology. 37:273 (Abstract). (DOI: http://dx.doi.org/10.1016/0093-691X(92)90342-O)
  24. Pontes JHF, Melo Sterza FA, Basso AC, Ferreira CR, Sanches BV, Rubin KCP and Seneda MM. 2011. Ovum pick up, in vitro embryo production, and pregnancy rates from a largescale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology. 75:1640-6. (DOI:10.1016/j.theriogenology.2010.12.026)
  25. Rosenkrans CF JR, Zeng GQ, McNamara GT, Schoff PK and First NL. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod. 49:459-62. (DOI:10.1095/ biolreprod49.3.459 )
  26. Roth Z and Hansen PJ. 2004. Involvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation. Biol. Reprod. 71:1898-906. (DOI: 10.1095/ biolreprod.104.031690 )
  27. Roth Z, Inbar G and Arav A. 2008. Comparison of oocyte developmental competence and follicular steroid content of nulliparous heifers and cows at different stages of lactation. Theriogenology. 69:932-9. (DOI:10.1016/j.theriogenology.2008.02.001)
  28. Seidel GE. 1981. Superovulation and embryo transfer in cattle. Science. 211:351-8. (DOI: 10.1126/science.7194504 )
  29. Su L, Yang S, He X, Li X, Ma J, Wang Y, Presicce GA and Ji W. 2012. Effect of donor age on the developmental competence of bovine oocytes retrieved by ovum pick up. Reprod. Domest. Anim. 47:184-9. (DOI: 10.1111/j.1439-0531. 2009.01349.x)
  30. Van Wagtendonk-de Leeuw A. 2006. Ovum pick up and in vitro production in the bovine after use in several generations: a 2005 status. Theriogenology. 65:914-25. (DOI:10.1016/j.theriogenology.2005.09.007)
  31. 김성수, 최병현, 조현태, 진종인, 하아나, 민찬식, 조규완, 공일근. 2014. OPU 채란계절이 한우의 난자 품질 및 발달 능력에 미치는 영향. 한국수정란이식학회지 29:265-71. (http://dx.doi.org/10.12750/JET.2014.29.3.265)
  32. 김용준, 송재웅, 서세현, 장구남, 김용수, 이해리, 신동수, 조성우, 김수희. 2004. 한우 및 젖소에서 과배란 처리를 이용한 체내수정란 생산과 신선 및 동결 수정란 이식 결과. 한국수정란이식학회지 19:209-18.
  33. 손동수, 김일화, 류일선, 연성흠, 서국현, 이동원, 최선호, 박수봉, 이충섭, 최유림, 안병석, 김준식. 2000. 젖소 MOET Scheme의 추진을 위한 수정란 생산과 이식. 한국수정란이식학회지 15:57-65.
  34. 송상현, 장덕일, 민찬식, 박준규, 주영국, 이정규, 정기화. 2012. 과배란 처리된 한우의 수정란 생산에 미치는 산차와 계절의 효과. 한국수정란이식학회지 27:127-31.
  35. 신상민, 김용준, 이해리 신동수, 김용수, 김수희, 이영준. 2009. 한우의 반복 과배란 처리에 의한 체내 수정란의 생산과 이식. 한국수정란이식학회지 24:47-56.
  36. 이병천, 윤기영, 김정태, 이강남, 노상호, 신태영, 박종임, 김남렬, 주석천, 백남용, 이은송, 임정묵, 이우근, 황우석. 1998. 초음파 유도에 의한 소 난포란의 채취에 관한 연구. 2. 임신우 유래 난포란으로부터 산자생산에 관하여. 한국수정란이식학회지 13:77-86.
  37. 진종인, 권태현, 최병현, 김성수, 조현태, 방재일, 김삼철, 조규완, 이정규, 공일근. 2011. 초기 임신우의 공란우 활용이 초음파 유도 난자 채취 및 수정란 생산에 미치는 영향. 한국수정란이식학회지 26:19-25.
  38. 진종인, 최병현, 김성수, 조현태, 선두원, 임현태, 이정규, 민찬식, 공일근. 2014. OPU 유래 한우 수정란 생산 및 이식. 한국수정란이식학회지 29:273-81.
  39. 최병현, 진종인, 권태현, 김성수, 조현태, 공일근, 2011. 한우와 젖소 대리모가 OPU 유래 한우 송아지의 체중과 임신기간에 미치는 영향. 한국수정란이식학회지 26:27-32.
  40. 최선호, 류일선, 손동수, 조상래, 한만회, 김현종, 최창용, 김영근. 2005. 한우의 반복 과배란 처리 및 산차가 수정란 생산에 미치는 영향. 한국수정란이식학회지 20:185-90.