Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Nonthermal Sterilization of Pathogenic Escherichia coli by Intense Pulsed Light Using a Batch System

회분식 광펄스 처리에 의한 병원성 대장균의 비가열 살균

  • Received : 2014.10.01
  • Accepted : 2014.11.23
  • Published : 2015.02.28
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Abstract

Intense pulsed light (IPL), a nonthermal technology, has attracted increasing interest as a food processing technology. However, its efficacy in inactivating microorganisms has not been evaluated thoroughly. In this study, we investigated the influence of IPL treatment on the inactivation of Escherichia coli O157:H7 depending on light intensity, treatment time, and pulse number. Increased light intensity from 500 V to 1,000 V, raised the inactivation rate at room temperature. At 1000 V, the cell numbers were reduced by 7.1 log cycles within 120 s. In addition, increased pulse number or decreased distance between the light source and sample surface also led to an increase in the inactivation rate. IPL exposure caused a significant increase in the absorption at 260 nm of the suspending agent used in our experiments. This indicates that IPL-treated cells were damaged, consequently releasing intracellular materials. The growth of IPL-irradiated cells were delayed by about 5 h. The degree of damage to the cells after IPL treatment was confimed by transmission electron microscopy.

광펄스 기술의 주요 공정 변수인 빛의 세기, 펄스 수, 광원과 시료사이의 거리에 따른 병원성 대장균의 사멸효과, 형태학적 변화와 세포의 손상 및 회복 여부를 알아보았다. 1,000 V, 5 pps, 7.9 cm에서 병원성 대장균을 광펄스 처리하였을 경우 처리시간이 증가함에 따라 사멸정도가 증가하였으며, 120초 처리하였을 때 약 7.1 log CFU/mL 감소하였고, 사멸속도는 $0.07s^{-1}$이었다. 같은 빛의 세기에서는 펄스수가 증가할수록 사멸정도가 증가하였으며, 램프와 시료의 거리가 가까울수록 사멸효과가 높았다. 광펄스 처리에 의한 미생물의 사멸은 세포막의 파괴에 의한 것으로 처리 시간이 증가함에 따라 세포내 물질의 외부 유출이 증가하며, 사멸되지 않은 세포라도 손상을 입어 정상적인 생육을 하지 못하는 것으로 보아 사멸이 물리적 손상 이외에의 생물학적 손상이 있는 것으로 판단된다. 세포의 물리적 손상 여부를 전자 현미경으로 살펴본 결과 광펄스 처리를 받은 세포는 세포막에 손상을 입어 세포내 물질의 유출이 일어난 것을 알 수 있었다.

Keywords

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