Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Pre-validation of Colony Forming Efficiency Assay for Assessing the Cytotoxicity of Nanomaterials

나노물질의 세포독성 평가법으로 Colony Forming Efficiency Assay에 대한 검증연구

  • Jo, Eunhye (National Institute of Environmental Research) ;
  • Lee, Jaewoo (National Institute of Environmental Research) ;
  • Park, Sun-Young (National Institute of Environmental Research) ;
  • Kim, Pilje (National Institute of Environmental Research) ;
  • Choi, Kyunghee (National Institute of Environmental Research) ;
  • Eom, Igchun (National Institute of Environmental Research)
  • Received : 2014.12.04
  • Accepted : 2015.01.30
  • Published : 2015.02.28
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Abstract

Objectives: The cytotoxcities of Au, Ag, SWCNT, $SiO_2$, and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and $SiO_2$ nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.

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References

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