Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Change of population density of tobacco whitefly (Bemisia tabaci, Aleyrodidae, Hemiptera) by RNAi

RNAi에 의한 담배가루이(Bemisia tabaci, 가루이과, 노린재목)의 개체군 밀도변화

  • Ko, Na-Yeon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young-Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 고나연 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2015.02.23
  • Accepted : 2015.03.12
  • Published : 2015.03.31
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Abstract

Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

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References

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