The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Physiological Activities of Ginkgo biloba Sarcotesta Extract with Heat Treatment

열처리에 따른 은행 외종피 추출물의 생리활성

  • Kim, Sung Tae (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Ji Hyun (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Sang Hoon (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Li, Meishan (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Min Young (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Yoon, Nara (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Biotechnology, Chungbuk National University)
  • 김성태 (충북대학교 식품생명공학과) ;
  • 이지현 (충북대학교 식품생명공학과) ;
  • 이상훈 (충북대학교 식품생명공학과) ;
  • 장귀영 (충북대학교 식품생명공학과) ;
  • ;
  • 김민영 (충북대학교 식품생명공학과) ;
  • 윤나라 (충북대학교 식품생명공학과) ;
  • 이준수 (충북대학교 식품생명공학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Received : 2015.04.28
  • Accepted : 2015.06.09
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the physiological activities of Ginkgo biloba sarcotesta extracts before and after heat treatment. G. biloba sarcotesta was heated at $130^{\circ}C$ for 2 h and extracted with water, 70% ethanol and 80% methanol. ABTS and DPPH radical scavenging activities increased after heating in the water (14.95 mg AAE/g and 7.36 mg TE/g) and ethanol extracts (12.20 mg AAE/g and 6.23 mg TE/g). ${\alpha}$-Glucosidase inhibitory activity decreased after heating in all but the water extract. Angiotensin converting enzyme I inhibitory activities decreased after heating in all extracts. Nitric oxide production inhibitory activity increased from 12.40~44.55% of the raw sample to 40.76~72.39% of the heated sample at a concentration of $200{\mu}g/mL$. Lipid accumulation inhibitory activities were similar before and after heat treatment. The highest antiproliferative effects on MCF-7 human breast cancer cell lines were observed in 80% methanol extract in the heated sample. Cell viability at concentrations of 25, 50, 100, and $200{\mu}g/mL$ measured 34.88, 17.58, 8.44 and 10.48%, respectively. From the results, the antioxidant and antiproliferative activities of G. biloba sarcotesta extracts increased with heat treatment, and research on the identification of the structure for the active compounds are needed in further studies.

은행 가공산업에서 부산물로 제거되는 은행 외종피의 활용성을 증대시키고자 다양한 생리활성에 미치는 열처리의 영향을 살펴보았다. 은행 외종피를 분리하여 $130^{\circ}C$에서 2시간 열처리한 후 물, 70% 에탄올 및 80% 메탄올 추출물을 제조하여 생리활성변화를 살펴본 결과, ABTS와 DPPH 자유라디칼 소거활성은 80% 메탄올 추출물을 제외하고, 열처리 후 증가하는 경향을 보였으며, 물 추출물에서 각각 14.95 mg AAE/g과 7.36 mg TE/g으로 높게 나타났다. ${\alpha}$-Glucosidase 저해 활성은 물 추출물을 제외하고 열처리 시 감소하는 경향을 보였으며, 열처리 전 80% 메탄올 추출물에서 98.66%로 가장 높게 나타났다. Angiotensin converting enzyme I 저해 활성은 추출물 간의 유의적인 차이가 없었으며(P>0.05), 열처리 후 모두 감소하였다. 산화질소 생성억제 활성은 열처리 시 증가하였으며, 물 추출물($200{\mu}g/mL$)이 $12.33{\mu}M$로 LPS 처리구에 비해 산화질소 생성량을 72.39% 감소시켰다. 지방구 축적억제 활성은 세포독성을 보이지 않는 농도에서 열처리 시 감소하였다. 유방암 세포주에 대한 항암 활성은 열처리 후 증가하는 경향을 보였다. 이상의 결과로부터 은행 외종피 추출물의 다양한 생리활성을 확인하였으며, 특히 열처리 후 항산화 활성과 유방암 세포주에 대한 증식억제 활성이 크게 증가하였으며, 추후 생리활성물질의 규명 연구가 필요하다고 판단된다.

Keywords

References

  1. Birkle D, Kurian P, Braquet P, Bazan N. 1998. Platelet-activation factor antagonist BN 52021 decrease accumulation of free polyunsaturated fatty acid in mouse brain during ischemia and electroconvulsive shock. J Nurochem 51:1900-1905
  2. Bombardelli E, Seplta M. 1990. Ginko biloba: chemistry and therapy. 2nd seminar on "Utilization of botanical derivatives in pharmaceuticals, cosmetics and para-pharmaceuticals"
  3. Choi JG, Jeong SI, Ku CS, Sathishkumar M, Lee JJ, Mun SP, Kim SM. 2009. Antibactrial activity of hydroxyalkenyl salicylic acids from sarcotesta of Ginkgo biloba against vancomycinresistant Enterococcus. Fitoterapia 80:18-20 https://doi.org/10.1016/j.fitote.2008.09.001
  4. Choi Y, Kim MH, Shin JJ, Park JM, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32:723-727 https://doi.org/10.3746/jkfn.2003.32.5.723
  5. Chung BS, Shin MG. 1990. Atlas of Herbal Medicine. Young Lim Sa, Seoul, Korea. pp. 122-124
  6. Cushman DW, Cheung HS. 1971. Spectophotometric assay and properties of the angiotensin converting enzyme of rabbit lung. Biochem Pharmacol 20:1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  7. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010-3014 https://doi.org/10.1021/jf0115589
  8. Goh LM, Barlow PJ. 2002. Antioxidant capacity of Ginkgo biloba. Food Res Int 35:815-820 https://doi.org/10.1016/S0963-9969(02)00084-4
  9. Han SH, Woo NRY, Lee SD, Kang MH. 2006. Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean J Medicinal Crop Sci 14:49-55
  10. Hwang IG, Kim HY, Woo KS, Lee J, Jeong HS. 2011a. Biological activities of Maillard reaction products (MRPs) in a sugar-amino acid model system. Food Chem 126:221-227 https://doi.org/10.1016/j.foodchem.2010.10.103
  11. Hwang IG, Kin HY, Woo KS, Hong JT, Hwang BY, Jung JK, Lee J, Jeong HS. 2011b. Isolation and characterisation of an ${\alpha}$-glucosidase inhibitory substance from fructose-tyrosine Maillard reaction products. Food Chem 127:122-126 https://doi.org/10.1016/j.foodchem.2010.12.099
  12. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical chracteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38:342-347
  13. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. 1996. A combined assay of cell viability and in vitro cytotoxicity with highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharm Bull 19:1518-1520 https://doi.org/10.1248/bpb.19.1518
  14. Jang GW, Kim HY, Lee SH, Kang YR, Hwang IG, Woo KS, Kang TS, Lee J, Jeong HS. 2012. Effects of heat treatment and extraction method on antioxidant activity of several medicine plants. J Korean Soc Food Sci Nutr 41:914-920 https://doi.org/10.3746/jkfn.2012.41.7.914
  15. Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. 2008. Effets of heat treatments on the antioxidant activities of fruits and vegetables. Korean J Food Sci Technol 40:166-170
  16. Kim JM, Shu Ds, Kim YS, Kim KO. 2004. Physical and sensory properties of rice gruels and cakes containing different level of ginkgo nut powder. Korean J Food Sci Technol 36:410-415
  17. Kim ST. 2015. Physiological activities of heated Ginkgo biloba sarcotesta and identification of antiproliferative substances. M.S. Thesis, Chungbuk National Univ. Chungbuk. Korea.
  18. Kim YM, Wang MH, Rhee HI. 2004. A novel ${\alpha}$-glucosidase inhibitor from pine bark. Carbohydr Res 339:715-717 https://doi.org/10.1016/j.carres.2003.11.005
  19. Lee SH, Hwang IG, Lee YR, Jeong EM, Jeong HS, Lee HB. 2009. Physicochemical characteristics and antioxidant activity of heated radish (Raphanus sativus L.) extracts. J Korean Soc Food Sci Nutr 38:490-495 https://doi.org/10.3746/jkfn.2009.38.4.490
  20. Park DK, Park HJ. 1990. Studies on the effects of rat platelet aggregation by Ginkgo and Perilla oil dietary. J Korean Soc Food Nutr 19:127-132
  21. Park E, Kum S, Wang C, Park SY, Kim BS, Schuller-Levis G. 2005. Anti-inflammatory activity of herbal medicines: inhibition of nitric oxide production and tumor necrosis factoralpha secretion in an activated macrophage-like cell line. Am J Chin Med 33:415-424 https://doi.org/10.1142/S0192415X05003028
  22. Park SB, Cho GS. 2011. Antimicrobial activity of extracts and fractions of Ginkgo biloba leaves, seed and outer seedcoat. J Jorean Soc Food Sci Nutr 40:7-13 https://doi.org/10.3746/jkfn.2011.40.1.007
  23. Pinto MS, Know YI, Apostolidis E, Lajolo FM, Genovese MI, Shetty K. 2009. Potential of Ginkgo biloba L. leave in the management of hyperglycemia and hypertension using in vitro model. Bioresour Technol 100:6599-6609 https://doi.org/10.1016/j.biortech.2009.07.021
  24. Singh B, Kaur P, Gopichand, Singh RD, Ahuja PS. 2008. Biology and chemistry of Ginkgo biloba. Fitoterapia 79:401-418 https://doi.org/10.1016/j.fitote.2008.05.007
  25. Wu X, Mao G, Zhao T, Zhao J, Li F, Liang L, Yang L. 2011. Isolation, purification and in vitro anti-tumor activity of polysaccharide from Ginko biloba sarcotesta. Carbohydr Polym 86:1073-1076 https://doi.org/10.1016/j.carbpol.2011.04.069
  26. Yang JT, Wu CE, Li YY, Jia SQ, Fan GJ, Fang R. 2011. Identification and purification of an allergic glycoprotein from Ginkgo biloba kernel. Agricultual Sciences in China 10:631-641 https://doi.org/10.1016/S1671-2927(11)60045-X

Cited by

  1. 조리조건에 따른 은행알의 4'-O-methylpyridoxine (ginkgotoxin) 함량 및 항산화 활성 변화 vol.49, pp.5, 2015, https://doi.org/10.9721/kjfst.2017.49.5.532