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Effect of Monascus-Fermentation on the Content of Bioactive Compounds in White and Black Soybeans

홍국발효가 백태와 서리태의 생리활성 성분에 미치는 영향

  • Jin, Yoo-Jeong (Department of Food and Nutrition, Sungshin Women's University) ;
  • Pyo, Young-Hee (Department of Food and Nutrition, Sungshin Women's University)
  • 진유정 (성신여자대학교 식품영양학과) ;
  • 표영희 (성신여자대학교 식품영양학과)
  • Received : 2015.03.21
  • Accepted : 2015.05.01
  • Published : 2015.06.30

Abstract

Changes in the contents of mevinolins (natural statins, $0-568.18{\mu}g$), coenzyme Q10 (CoQ10, $26.41-65.59{\mu}g$), and tocopherols ($232.80-312.87{\mu}g/g$ dry weight) in Monascus-fermented soybean were determined using HPLC. Significant increases (p<0.05) in mevinolins and CoQ10 were obtained in Monascus-fermented soybean after 20 days of fermentation compared with unfermented soybean (0 days), whereas no significant change (p>0.05), or a slight decrease, in tocopherols was observed. The results indicate that Monascus-fermentation has great potential for enriching mevinolin and CoQ10 in soybeans.

Monascus pilosus KCCM 60084를 백태와 서리태에 각각 접종시켜 $30^{\circ}C$에서 발효한 후, 홍국발효 콩에 함유된 미량의 생리활성물질에 대한 영향을 측정하였다. 천연 스타틴 성분인 mevinolin은 발효 전의 미 검출에서 홍국 발효 20일 후에 건조시료 g당(${\mu}g/g$ DW) 최대 $568.18{\mu}g$ 생산하였고, CoQ10의 함량은 발효전의 농도에 비해 발효 20일 후에 백태와 서리태 모두 2배 이상 증가하여 각각 $65.59{\pm}9.53$$54.92{\pm}5.27{\mu}g$으로 나타났다. 토코페롤 함량은 홍국발효에 의해 뚜렷한 변화가 관찰되지 않았다. 발효 전의 백태에 함유된 총 토코페롤 함량은 $312.87{\mu}g$으로 서리태의 $256.76{\mu}g$ 보다 약 22% 더 높았으며(p<0.05), 가장 많이 함유된 토코페롤 종류는 ${\gamma}$-토코페롤로 전체 농도의 약 55%을 차지 하였다. 따라서 홍국발효는 콩의 메비놀린과 CoQ10 성분의 농도를 발효과정을 통해 변화시키는 것으로 나타났다.

Keywords

References

  1. Martinkova L, Juzlova P, Vesely D. Biological activity of polyketide pigments produced by the fungus Monascus. J. Appl. Bacteriol. 79: 609-616 (1995). https://doi.org/10.1111/j.1365-2672.1995.tb00944.x
  2. Ma J, Li Y, Ye Q, Li J, Hua Y, Ju D, Zhang D, Cooper R, Chang M. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agr. Food Chem. 48: 5220-5225 (2000) https://doi.org/10.1021/jf000338c
  3. Monacolin K. A new hypocholesterolemic agent produced by a Monascus species Endo. J. Antibiot. 32: 852-854 (1979) https://doi.org/10.7164/antibiotics.32.852
  4. Wild D, Toth G, Humpf HU. New Monascus metabolite isolated from red yeast rice (angkak, red koji). J. Agr. Food Chem. 50: 3999-4002 (2002) https://doi.org/10.1021/jf020023s
  5. Rallidis LS, Fountoulaki K, Anastasiou-Nana M. Managing the underestimated risk of statin-associated myopathy. Int. J. Cardiol. 159: 169-176 (2012) https://doi.org/10.1016/j.ijcard.2011.07.048
  6. Marcoff L, Thompson PD. The role of coenzyme Q10 in statinassociated myopathy: A systematic review. J. Am. Coll. Cardiol. 49: 2231-2237 (2007) https://doi.org/10.1016/j.jacc.2007.02.049
  7. Schaefer WH, Lawrencea JW, Loughlina AF, Stoffregena DA, Mixsonb LA, Deanc DC, Raabc CE, Yuc NX, Lankasa GR, Frederick CB. Evaluation of ubiquinone concentration and mitochondrial function relative to cerivastatin-induced skeletal myopathy in rats. Toxicol. Appl. Pharm. 194: 10-23 (2004) https://doi.org/10.1016/j.taap.2003.08.013
  8. Bentinger M, Tekle M, Dallner G. Coenzyme Q-biosynthesis and functions. Biochem. Bioph. Res. Co. 396: 74-79 (2010) https://doi.org/10.1016/j.bbrc.2010.02.147
  9. Niklowitz P, Sonnenschein A, Janetzky B, Andler W, Menke T. Enrichment of coenzyme Q10 in plasma and blood cells: Defense against oxidative damage. Int. J. Biol. Sci. 3: 257-262 (2007)
  10. Pyo YH, Seo SY. Simultaneous production of natural statins and coenzyme $Q_{10}$ by Monascus pilosus fermentation using different solid substrates. Food Sci. Biotechnol. 19: 1635-1641 (2010) https://doi.org/10.1007/s10068-010-0231-7
  11. Journoud M, Jones PJH. Red yeast rice: A new hypolipidemic drug. Life Sci. 74: 2675-2683 (2004) https://doi.org/10.1016/j.lfs.2003.10.018
  12. Hong MY, Seeram NP, Zhang Y, Heber D. Anticancer effects of Chinese red yeast rice versus monacolin K alone on colon cancer cells. J. Nutr. Biochem. 19: 448-458 (2008) https://doi.org/10.1016/j.jnutbio.2007.05.012
  13. Friedrich J, Zuzek M, Bencina M, Cimerman A, Strancar A, Radez I. High- performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths. J. Chromatogr. A. 704: 363-367 (1995) https://doi.org/10.1016/0021-9673(95)00096-6
  14. Pyo YH, Oh HJ. Ubiquinone contents in Korean fermented foods and average daily intakes. J. Food Compos. Anal. 24: 1123-1129 (2011) https://doi.org/10.1016/j.jfca.2011.03.018
  15. Slavin M, Yu L. A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans. Food Chem. 135: 2789-2795 (2012) https://doi.org/10.1016/j.foodchem.2012.06.043
  16. Pyo YH, Lee YC. Mevinolin production by Monascus pilosus IFO 480 in solid state fermentation of soymeal. Food Sci. Biotechnol. 15: 647-649 (2006)
  17. Juzlova P, Martinkova L, Kren V. Secondary metabolites of the fungus Monascus: A review. J. Ind. Microbiol. 16: 163-170 (1996) https://doi.org/10.1007/BF01569999
  18. Barrios-Gonzalez J, Fernandez FJ, Tomasini A, Mejia A. Secondary metabolites production by solid-state fermentation. Malays. J. Microbiol. 1: 1-6 (2005)
  19. Littarru GP, Tiano L. Bioenergetic and antioxidant properties of coenzyme $Q_{10}$: Recent developments. Mol. Biotechnol. 37: 31-37 (2007) https://doi.org/10.1007/s12033-007-0052-y
  20. Mattila P, Kumpulainen J. Coenzymes $Q_9$ and $Q_{10}$: Contents in foods and dietary intake. J. Food Compos. Anal. 14: 409-417 (2001) https://doi.org/10.1006/jfca.2000.0983
  21. Rodriguez-Acuna R, Brenne E, Lacoste F. Determination of coenzyme $Q_{10}$ and $Q_9$ in vegetable oils. J. Agr. Food Chem. 56: 6241-6245 (2008) https://doi.org/10.1021/jf800103e
  22. Bramley PM, Elmadfa I, Kafatos A, Kelly FJ, Manios Y, Roxborough HE, Schuch W, Sheehy PJA, Wagner KH. Vitamin E: Tocopherols and tocotrienols. J. Sci. Food Agr. 80: 913-938 (2000) https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<913::AID-JSFA600>3.0.CO;2-3
  23. Tucker JM, Townsend DM. Alpha-tocopherol: Roles in prevention and therapy of human disease. Biomed. Pharmacother. 59: 380-387 (2005) https://doi.org/10.1016/j.biopha.2005.06.005
  24. Pacanowski MA, Frye RF, Enogieru O, Schofield RS, Zineh I. Plasma coenzyme $Q_{10}$ predicts lipid-lowering response to highdose atorvastatin. J. Clin. Lipidol. 2: 289-297 (2008) https://doi.org/10.1016/j.jacl.2008.05.001
  25. Jiang Q, Christen S, Shigenaga MK, Ames BN. ${\gamma}$-Tocopherol, the major form of vitamin E in the US diet, deserves more attention. Am. J. Clin. Nutr. 74: 714-722 (2001) https://doi.org/10.1093/ajcn/74.6.714
  26. Chung IM, Yong SJ, Lee JH, Kim SH. Effect of genotype and cultivation location on ${\beta}$-sitosterol and ${\alpha}$-, ${\beta}$-, ${\gamma}$-, and ${\delta}$-tocopherols in sorghum. Food Res. Int. 51: 971-976 (2013) https://doi.org/10.1016/j.foodres.2013.02.027
  27. Guzman GJ, Murphy PA. Tocopherols of soybean seeds and soybean curd (tofu). J. Agr. Food Chem. 34: 791-795 (1986) https://doi.org/10.1021/jf00071a006
  28. Yang JH, Tseng YH, Lee YL, Mau JL. Antioxidant properties of methanolic extracts from monascal rice. LWT-Food Sci. Technol. 39: 740-747 (2006) https://doi.org/10.1016/j.lwt.2005.06.002

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