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Analytical Characteristics of GC/MS and HPLC according to the Concentration Distribution of PAHs

PAHs 농도 분포에 따른 GC/MS와 HPLC의 분석특성에 관한 연구

  • Hong, Jwa-Ryung (Samsung Health Research Institute, Samsung Electronics Co. Ltd.) ;
  • Choi, Kwang-Min (Samsung Health Research Institute, Samsung Electronics Co. Ltd.)
  • Received : 2015.07.10
  • Accepted : 2015.09.16
  • Published : 2015.09.30

Abstract

Objectives: The purpose of this study was to determine the best method to analyze PAHs at extremely low concentrations. To this end, 16 PAHswere analyzed simultaneously by GC/MS, HPLC/FLD and HPLC/UVD, and the analytical characteristics of HPLC and GC/MS were compared. Methods: This study was conducted by GC/MS and HPLC/FLD/UVD, and evaluated linearity, precision and detection limit. Standard solutions were prepared for 21 samples in the range of $0.00001{\sim}1.0{\mu}g/mL$ and the samples were divided into four groups. All samples were made in three sets and analysis was replicated seven times. Results: Sixteen PAHs could be simultaneously separated by HPLC and GC/MS, and the adequate equipment was HPLC/FLD. The retention times by HPLC were shorter than GC/MS, and HPLC had better separation for most PAHs than GC/MS. The peaks of naphthalene and naphthalene-D8 partially overlapped for GC/MS. HPLC/FLD had a 20-2000 times lower limit of detection than GC/MS and UVD. However FLD was not adequate for analyzing acenaphthylene because it has too low a fluorescence quantum yield to be detected. The precision of HPLC/FLD/UVD and GC/MS showed less than 20% at $0.001{\mu}g/mL$ PAHs and when the concentration was higher, the coefficient of variation was decreased. HPLC/FLD was better for the overall detection of limits. Conclusions: The results indicate that the HPLC/FLD method has good linear range, precision and a detection of limits from $0.00001{\sim}0.0001{\mu}g/mL$ for all 16 PAHs. This study contributes to providing useful data for analysis technology and can be applied to occupational exposure measurement for PAHs in workplaces.

Keywords

References

  1. Agency for Toxic Substances and Disease Registry (ATSDR). U.S. Department of health and human services, Toxicological profile for polycyclic aromatic hydrocarbons, 1995
  2. American society for Testing and Materials(ASTM). The Annual Book of ASTM standards, D 4657-92, PA., U.S.A, 1-9, 1998
  3. Chen BH, Wang CY, Chiu CP. Evaluation of analysis of polycyclic aromatic hydrocarbons in meat products by liquid chromatography. J Agric Food Chem 1996; 44(8):2244-2251 https://doi.org/10.1021/jf9508211
  4. Chiu CP, Lin YS, Chen BH. Comparison of GC-MS and HPLC for overcoming matrix interferences in the anlaysis of PAHs in smoked food. J. Chromatogr 1997;44
  5. Crete J, Ingrid C, Jorgensen B, Rolf C. Quantitative determination of de-conjugated chrysene metabolites in fish bile by HPLC-fluorescence and GC-MS. Chemosphere 2004;54(8):1085-097
  6. Environmental Protection Agency(EPA). Code of Federal Regulation, title 40, part 60, subparts D, Da, Db, Dc. Washington, DC, 1997:44
  7. Environmental Protection Agency(EPA). Compendium Method TO-13A, EPA, Cincinnati, OH, USA, 1999
  8. Environmental Protection Agency (EPA). Compendium of methods for the determination of toxic organic compounds in ambient air. Second edition. EPA/625/R-96/010b, January 1999
  9. Gerbino TC, Castello G. Analysis of polycyclic aromatic hydrocatbons with an ion-trap mass detector and comparison with other gas chromatographic and high performance liquid chromatographic techniques. J. Chromatogr 1993;642:351-357 https://doi.org/10.1016/0021-9673(93)80098-S
  10. Gratz L, Bagley S, Leddy D, Johnson J, Johnson JH et al. Interlaboratory comparison of HPLC-fluorescence detection and GC/MS: analysis of PAH compounds present in diesel exhaust. J Hazardous Materials 2000;74:37-46 https://doi.org/10.1016/S0304-3894(99)00197-1
  11. Guilbault G. Practical Fluorecence, 2nd ed., revised and expanded; Marcel Dekker, New york. 1990;23
  12. Hu SJ, Oh NS, Kim SY, Lee HM. Determining of polycyclic aromatic hydrocarbons in domestic vegetables and fruits. Anal Sci Technol 2006;19(5):415-421
  13. International Agency Research on Cancer(IARC). IARC Monographs on the evaluation of carcinogenic risk of chemicals to human. 2006;7:1-42
  14. International Agency Research on Cancer(IARC). IARC Monographs in the evaluation of carcinogenic risks to humans. 2010
  15. International Conference Air Quality in Europe; Challenges for the 2000s, Cocheo V, Saeger E, kotzias D, Venice, 1999
  16. Jung JH, Cho SW, Lim HS. Distribution characteristics of environmental contaminant at soil in an industrial complex area. Clean technology 2011;17(4):379-388 https://doi.org/10.7464/KSCT.2011.17.4.379
  17. Jeon JM, Lim JY, Kim SM, Shin HS. Characteristics of polycyclic aromatic hydrocarbons from PM 2.5 in the public facilities. J Korean Soc Indoor Environ 2012;9(3):251-269
  18. Mastral AM, Callean MS. A Review on Polycyclic Aromatic Hydrocarbon(PAH) Emissions from Energy Generation. Environ Sci Technol 2000;34(15):3051-3057 https://doi.org/10.1021/es001028d
  19. Lee TJ, Park KY, Kim JB, Eun JE, Jeong WG et al. Determination of phenol using solid-phase extraction and high performance liquid chromatography/FLD/MSD in water. Rep Inst Health & Envion 2005;16: 144-122
  20. National Institute of Occupational Safety and Health (NIOSH). A NIOSH technical report: guidelines for air sampling and analytical method development and evaluation, NIOSH Pub. No. 95-117, Cincinnati, OH: NIOSH;1995.
  21. Raiyani CV, Shah SH, Desai NM, Venkaiah K, Patel JS et al. Characterization and problems of indoor pollution due to cooking stove smoke. Atmos Environ 27A 1993;1643-1655
  22. Williams R, Meares J, Brooks L, Watts R, Lemieux P. Priority pollutant PAH analysis of incinerator emission particles using HPLC and optimized fluorescence detection. Intern J Environ Ad Chem 1994;54: 299-314 https://doi.org/10.1080/03067319408034096
  23. Sloss L, Gardner CA. Sampling and analysis of trace emissions from coal-fired power stations, IEA Coal Research, IEARC 77: London, 1995
  24. Seo SJ, Seo YK, Hwang YJ, Jung DH, Baek SO. Application of adsorption sampling and thermal desorption with GC/MS analysis for the measurement of low-molecular weight PAHs in ambient air. J KOSAE 2014;30(4):362-77 https://doi.org/10.5572/KOSAE.2014.30.4.362
  25. Yoon JK, Park JS, Shin SK, Kim TS. Analytical method of polycyclic aromatic hydrocarbons (PAHs) in oil contaminated soils. Anal Sci Technol 2008;21(4): 296-303
  26. Zander M, Gollin G. A review of the significance of polycyclic aromatic chemistry for pitch science. Fuel 1993;72(9):1281-1285 https://doi.org/10.1016/0016-2361(93)90126-M

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