DOI QR코드

DOI QR Code

Preparation of Fermented Sauces with Increased ACE Inhibitory and Antioxidative Activities

ACE 저해능 및 항산화능이 향상된 발효소스의 제조

  • Received : 2015.06.09
  • Accepted : 2015.08.17
  • Published : 2015.10.31

Abstract

For development of premium sauce, fermented sauces containing ferment (with wheat flour and soybean) and extract(s) of Styela clava (Korean name: miduduk) tunic and/or mulberry leaves and/or onion were prepared, and their angiotensin converting enzyme (ACE) inhibitory and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were evaluated. All sauces containing extract(s) showed increased ACE inhibitory and DPPH radical scavenging activities. Especially, sauces containing extracts of mulberry leaves and onion showed superior activities, with 26.92% and 40.66% relatively increased ACE inhibitory and DPPH radical scavenging activities, respectively, compared to control (no extract was added). These results suggest that extracts of mulberry leaves and onion could improve antihypertensive and antioxidant activities of fermented sauce.

장류 발효기술과 유용 천연물을 활용한 프리미엄 발효소스의 개발을 목적으로 소맥분과 콩의 발효물(페이스트 형태), 미더덕 껍질, 뽕잎, 양파 추출물을 단독 또는 혼합하여 첨가한 발효소스를 제조한 후, ACE 저해능과 DPPH 라디칼 소거능을 측정하였다. 그 결과 무첨가 대조구에 비하여 추출물 첨가 발효소스의 ACE 저해능과 라디칼 소거능은 증가하였다. 특히 뽕잎과 양파 추출물을 혼합하여 첨가한 발효소스가 대조구에 비하여 상대적으로 ACE 저해능이 26.92% 증가하였고, DPPH 라디칼 소거능은 40.66% 증가하였다. 이러한 결과는 항고혈압능과 항산화능을 보유한 고품질 소스의 제조에 소맥분과 콩의 발효물, 뽕잎과 양파 추출물 등이 활용될 수 있음을 시사한다.

Keywords

References

  1. KFDA. Food Codex. http://fse.foodnara.go.kr/residue/RS/jsp/menu_02_01_03.jsp?idx=57 (accessed May 2015).
  2. Oh HS, Kim JH. 2006. Development of functional soy-based stew sauce including hot water extract of Cornus officinalis S. et Z. Korean J Food Culture 21: 550-558.
  3. Swales JD. 1984. Aetiology of hypertension. Br J Anaesth 56: 677-688. https://doi.org/10.1093/bja/56.7.677
  4. Cambien F, Chretien JM, Ducimetiere P, Guize L, Richard JL. 1985. Is the relationship between blood pressure and cardiovascular risk dependent on body mass index? Am J Epidemiol 122: 434-442. https://doi.org/10.1093/oxfordjournals.aje.a114124
  5. Weinberger MH. 1987. Angiotensin converting enzyme inhibitors in the treatment of hypertension: efficacy, metabolic effects and side effects. Cardiovasc Drugs Ther 1: 9-13. https://doi.org/10.1007/BF02125828
  6. Oparil S, Schmieder RE. 2015. New approaches in the treatment of hypertension. Circ Res 116: 1074-1095. https://doi.org/10.1161/CIRCRESAHA.116.303603
  7. Jung SY, Lim JS, Song HS. 2012. Alcohol dehydrogenase activity and sensory evaluation of Hutgae (Hovenia dulcis Thunb) fruit soy sauce. Korean J Food & Nutr 25: 747-754. https://doi.org/10.9799/ksfan.2012.25.4.747
  8. Yin XF, Choi SK, Namkung Y. 2011. Quality characteristics of soy reducing sauce made with apple concentrate. J East Asian Soc Dietary Life 21: 823-829.
  9. Oh KH, Song HS. 2013. Sensory evaluation of seasoned soy sauce with Hutgae (Hovenia dulcis Thunb) fruit and pear extracts. Korean J Food & Nutr 26: 323-328. https://doi.org/10.9799/ksfan.2013.26.2.323
  10. Lee MS, Park ML, Jung HA, Choi SK. 2011. Quality characteristics of Gochujang dressing containing various amounts of Maesil (Prunus mune) concentrate. J East Asian Soc Dietary Life 21: 38-45.
  11. Joo NM, Jung HS, Yoon JY, Park SH, Lee SM, Song YH, Lee JH. 2010. Development and application of soybean paste sauce with walnuts and sesame seeds. Korean J Culinary Res 16: 298-306.
  12. Chang KH, Cho KH, Kang MK. 2012. Optimization of the preparation conditions and quality characteristics of sweet pumpkin-doenjang sauce. Korean J Food Preserv 19: 492-500. https://doi.org/10.11002/kjfp.2012.19.4.492
  13. Shin YJ, Lee CK, Kim HJ, Kim HS, Seo HG, Lee SC. 2014. Preparation and characteristics of low-salt soy sauce with anti-hypertensive activity by addition of miduduk tunic, mulberry, and onion extracts. J Korean Soc Food Sci Nutr 43: 854-858. https://doi.org/10.3746/jkfn.2014.43.6.854
  14. KFDA. Food Codex. http://fse.foodnara.go.kr/residue/RS/jsp/menu_02_01_01.jsp (accessed May 2015).
  15. Cushman DW, Cheung HS. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9
  16. Jeong SM, Kim SY, Park HR, Lee SC. 2004. Effect of far-infrared radiation on the activity of extracts from Citrus unshiu peels. J Korean Soc Food Sci Nutr 33: 1580-1583. https://doi.org/10.3746/jkfn.2004.33.9.1580
  17. SPSS. 2006. SPSS 14.0 for Windows. SPSS Inc., Chicago, IL, USA.
  18. Lee SM, Chang HC. 2009. Growth-inhibitory effect of the solar salt-doenjang on cancer cells, AGS and HT-29. J Korean Soc Food Sci Nutr 38: 1664-1671. https://doi.org/10.3746/jkfn.2009.38.12.1664
  19. Ryu BH. 2003. Development of functional doenjang for antioxidative and fibrinolytic activity. Korean J Life Sci 13: 599-568.
  20. Corvol P, Michaud A, Soubrier F, Williams TA. 1995. Recent advances in knowledge of the structure and function of the angiotensin I converting enzyme. J Hypertens Suppl 13: S3-S10. https://doi.org/10.1097/00004872-199509003-00002
  21. Corvol P, Jeunemaitre X, Charru A, Kotelevtsev Y, Soubrier F. 1995. Role of the renin-angiotensin system in blood pressure regulation and in human hypertension: new insights from molecular genetics. Recent Prog Horm Res 50: 287-308.
  22. Lee DW, You DH, Yang EK, Jang IC, Bae MS, Jeon YJ, Kim SJ, Lee SC. 2010. Antioxidant and ACE inhibitory activities of Styela clava according to harvesting time. J Korean Soc Food Sci Nutr 39: 331-336. https://doi.org/10.3746/jkfn.2010.39.3.331
  23. Yang NC, Jhou KY, Tseng CY. 2012. Antihypertensive effect of mulberry leaf aqueous extract containing ${\gamma}$-aminobutyric acid in spontaneously hypertensive rats. Food Chem 132: 1796-1801. https://doi.org/10.1016/j.foodchem.2011.11.143
  24. Duarte J, Perez-Palencia R, Vargas F, Ocete MA, Perez-Vizcaino F, Zarzuelo A, Tamargo J. 2001. Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats. Br J Pharmacol 133: 117-124. https://doi.org/10.1038/sj.bjp.0704064
  25. Makino A, Skelton MM, Zou AP, Roman RJ, Cowley AW Jr. 2002. Increased renal medullary oxidative stress produces hypertension. Hypertension 39: 667-672. https://doi.org/10.1161/hy0202.103469
  26. Kizhakekuttu TJ, Widlansky ME. 2010. Natural antioxidants and hypertension: promise and challenges. Cardiovasc Ther 28: e20-e32. https://doi.org/10.1111/j.1755-5922.2010.00137.x
  27. Jung ES, Park E, Park HR, Lee SC. 2008. Antioxidant activities of extracts from parts of Styela clava. J Korean Soc Food Sci Nutr 37: 1674-1678. https://doi.org/10.3746/jkfn.2008.37.12.1674
  28. Kim SK, Kim SW, Noh SJ, Kim YJ, Kang JH, Lee SC. 2013. Qualities of konjac containing tunic extract from Styela clava. J Korean Soc Food Sci Nutr 42: 410-414. https://doi.org/10.3746/jkfn.2013.42.3.410
  29. Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y. 2006. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem 97: 25-31. https://doi.org/10.1016/j.foodchem.2005.03.019
  30. Ra KS, Suh HJ, Chung SH, Son JY. 1997. Antioxidant activity of solvent extract from onion skin. Korean J Food Sci Technol 29: 595-600.
  31. Formica JV, Regelson W. 1995. Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol 33: 1061-1080. https://doi.org/10.1016/0278-6915(95)00077-1