DOI QR코드

DOI QR Code

Physicochemical Properties and Antioxidant Activities of Loose-leaf Green Tea Commercially Available in Korea

국내 시판 잎차 형태 녹차의 이화학 특성 및 산화방지 활성

  • Received : 2015.04.20
  • Accepted : 2015.05.26
  • Published : 2015.08.31

Abstract

The objective of this study was to investigate the physicochemical and antioxidant properties of a variety of commonly consumed commercial green tea. Green tea samples with the same commercial name produced at different regions were analyzed. High-grade tea samples showed higher values of lightness (L) and greenness (-a). Additionally, compared to other varieties of teas, high-grade tea samples showed higher levels of catechin, gallocatechin gallate (GCg), epicatechin gallate (ECg), theanine, and methylxanthines and a lower level of epigallocatechin (EGC). The antioxidant activity of green tea was also investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical assays. High-grade teas were observed to have higher antioxidant activities. The results of this study indicate that the catechin content, such as EGCg, GCg, and ECg levels, was found to positively influence the total antioxidant activity of green tea.

제주, 보성, 하동산 차엽의 우전, 세작, 중작 및 대작 상품명을 쓰고 있는 시판 잎 녹차의 색은 녹차 등급이 높을수록 즉 채엽시기가 빠를수록 L값과 녹색도인 -a값이 높게 나타났으며 제주산 녹차가 보성 및 하동산 녹차에 비해 L값과 b값이 높은 것으로 나타났다. 카테킨 분석 결과 EGC 함량은 차엽 산지에 관계없이 채엽시기가 늦어짐에 따라 유의적으로 증가하였으며, 카테킨, GCg, ECg 함량은 유의적으로 감소하였다. 카테킨류 중 EGCg 함량이 95.39-116.25 mg/g으로 가장 높았으며 특히 보성산 녹차가 다른 지역에 비해 유의적으로 높게 나타났다. 카페인, 테오브로민 및 테아닌 함량은 모든 차엽에서 채엽시기에 따라 유의적으로 감소하는 경향을 보였으며 특히 제주산 우전의 카페인, 테오브로민 및 테아닌 함량은 각각 33.43, 17.84, 20.84 mg/g으로 가장 높게 함유되어 있었다. 산화방지 활성은 측정방법에 상관없이 등급이 높은 우전에서 높게 나타났으며 제주 및 하동산 녹차의 경우 채엽시기가 늦어짐에 따라 유의적으로 감소하였으나 보성산 녹차는 채엽시기에 따라 유의적으로 증가하였다. 녹차의 주요 산화방지 성분인 개별 카테킨 함량과 산화방지 활성과의 상관관계를 분석한 결과 DPPH 활성은 EGCg, ECg 및 총카테킨 함량과 양의 상관관계가 있는 것으로 나타났으며 ABTS 활성은 EGC 함량과는 음의 상관관계 그리고 카테킨, GCg 및 ECg 함량과는 양의 상관관계가 있는 것으로 나타났다.

Keywords

References

  1. Cabrera C, Artacho R. Gime'nez R. Beneficial effects of green tea-A review. J. Am. Coll. Nutr. 25: 79-99 (2006) https://doi.org/10.1080/07315724.2006.10719518
  2. Khan N, Mukhtar H. Tea polyphenols for health promotion. Life Sci. 81: 519-533 (2007) https://doi.org/10.1016/j.lfs.2007.06.011
  3. Chung DH. Components and Efficacy of Tea. Hongikjae, Seoul, Korea. pp. 43-46 (2004)
  4. Khokhar S, Magnusdottir SGM. Total phenol, catechin, and caffeine contents of teas commonly consumed in the United Kingdom. J. Agr. Food Chem. 50: 565-570 (2002) https://doi.org/10.1021/jf010153l
  5. Lin YS, Tsai YJ, Tsay JS, Lin JK. Factors affecting the levels of tea polyphenols and caffeine in tea leaves. J. Agr. Food Chem. 51: 1864-873 (2003) https://doi.org/10.1021/jf021066b
  6. Sabhapondit S, Karak T. Bhuyan LP. Goswami BC. Hazarika M. Diversity of catechin in northeast Indian tea cultivars. Sci. World J. 2012: 1-8 (2012).
  7. Lee JY, Wang LF, Baik JH, Park SK. Changes in volatile compounds of green tea during growing season at different culture areas. Korean J. Food Sci. Technol. 39: 246-254 (2007)
  8. Oh MJ, Hong BH. Variation in chemical components of Korean green tea (Camellia sinensis L.) resulted from developing stages and processing recipe. Korean J. Crop Sci. 40: 518-524 (1995)
  9. Oh MJ, Hong BH. Variation of pectin, catechins and caffein contents in Korean green tea (Camellia sinensis L.) by harvesting time and processing Recipe. Korean J. Crop Sci. 40: 775-781 (1995)
  10. Wee JH, Moon JH, Park KH. Catechin content and composition of domestic tra leaves at different plucking time. Korean J. Food Sci. Technol. 31: 20-23 (1999)
  11. Kim SH, Han DS, Park JD. Changes of some chemical compounds of Korean (Posong) green tea according to harvest periods. Korean J. Food Sci. Technol. 36: 542-546 (2004)
  12. Zhu H, Kim JS, Park KL, Cho CW, Kim YS, Kim JW, Ryu SY, Kang JS. Estimation of harvest period and cultivated region of commercial green tea by pattern recognition. Yakhak Hoeji 53: 51-59 (2009)
  13. Lee LS, Kim SH, Kim YB, Kim YC. Quantitative analysis of major constituents in green tea with different plucking periods and their antioxidant activity. Molecules 19: 9173-9186 (2014) https://doi.org/10.3390/molecules19079173
  14. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 25-30 (1995) https://doi.org/10.1016/S0023-6438(95)80008-5
  15. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos. Anal. 19: 669-675 (2006) https://doi.org/10.1016/j.jfca.2006.01.003
  16. Hirun S, Utama-ang N, Vuong QV, Scarlett CJ. Investigating the Commercial microwave vacuum drying conditions on physicochemical properties and radical scavenging ability of Thai green tea. Dry. Technol. 32: 47-54 (2014) https://doi.org/10.1080/07373937.2013.811249
  17. Lee LS, Choi JH, Son N, Kim SH, Park JD, Jang DJ, Kim HJ. Metabolomic analysis of the effect of shade treatment on the nutritional and sensory qualities of green tea. J. Agr. Food Chem. 61: 332-338 (2013) https://doi.org/10.1021/jf304161y
  18. Park KR, Lee SG, Nam TG, Kim YJ, Kim YR, Kim DO. Comparative analysis of catechins and antioxidant capacity in various grades of organic green teas grown in Boseong, Korea. Korean J. Food Sci. Technol. 41: 82-86 (2009)
  19. Chen CN. Liang CM. Lai JR. Tsai YJ, Tsay JS, Lin JK. Capillary electrophoretic determination of theanine, caffeine, and catechins in fresh tea leaves and oolong tea and their effects on rat neurosphere adhesion and migration. J. Agr. Food Chem. 51: 7495-7503 (2003) https://doi.org/10.1021/jf034634b
  20. Lin YL. Juan IM. Chen YL Liang YC, Lin JK. Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells. J. Agr. Food Chem. 44: 1387-1394 (1996) https://doi.org/10.1021/jf950652k
  21. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agr. Food Chem. 53: 1841-1856 (2005) https://doi.org/10.1021/jf030723c

Cited by

  1. The Change of Catechin and Theanine Content in Green Tea during Different Storage Conditions vol.22, pp.5, 2016, https://doi.org/10.20878/cshr.2016.22.5.020
  2. Quality Characteristics of Horse Oil Extracted by Hot Water with Adding Green Tea Leaves vol.22, pp.3, 2018, https://doi.org/10.13050/foodengprog.2018.22.3.242