DOI QR코드

DOI QR Code

Analysis of Total Phenol, Flavonoid content and Antioxidant Activity of Various Extraction Solvents Extracts from Onion (Allium cepa L.) Peels

  • Duan, Yishan (Department of Food Science and Technology, Pusan National University) ;
  • Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University) ;
  • Seong, Jong-Hwan (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Young-Guen (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Dong-Seob (Department of Food Science and Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science and Technology, Pusan National University) ;
  • Jang, Seong-Ho (Department of Bioenvironmental Energy, Pusan National University)
  • Received : 2015.08.20
  • Accepted : 2015.09.03
  • Published : 2015.09.30

Abstract

Total phenol contents, total flavonoid contents and antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from onion (Allium cepa L.) peels were studied. The $IC_{50}$ values of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity in 70% ethanol extract were remained to be lowest followed by 70% methanol extract and CM extract. And the total phenol content ($113.56{\pm}0.86mg\;CAE/g$), total flavonoid content (49.63 mg QE/g) and ferric reducing antioxidant power value were also found to be the highest. In contrast, 70% methanol extract possessed the strongest antioxidant activity by ${\beta}$-carotene bleaching assay. CM extract displayed the lowest antioxidant activity compared with other extracts. Onion peels exhibited strong antioxidant activity and abundant phytochemicals, which could be used in a various food products to add phytochemicals and promote good health.

Keywords

References

  1. M. S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199 (1958). https://doi.org/10.1038/1811199a0
  2. T. Bahorun, A. Luximon-Ramma, A. Crozier and O. I. Aruoma, Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables, J. Sci. Food Agric., 84, 1553 (2004). https://doi.org/10.1002/jsfa.1820
  3. A. W. Boots, G. R. M. M. Haenen and A. Bast, Health effects of quercetin: From antioxidant to nutraceutical, European J. Pharmacol., 585, 325 (2008). https://doi.org/10.1016/j.ejphar.2008.03.008
  4. I. F. F. Benzie and J. J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Anal. Biochem., 239, 70 (1996). https://doi.org/10.1006/abio.1996.0292
  5. E. C. Cadenas and K. J. A. Davies, Mitochondrial free radical generation, oxidative stress, and aging, Free Radical Biol. Med., 29, 222 (2000). https://doi.org/10.1016/S0891-5849(00)00317-8
  6. Q. D. Do, A. E. Angkawijaya, P. L. Tran-Nguyen, L. H. Huynh, F. E. Soetaredjo, S. Ismadji and Y. H. Ju, Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic, J. Food Drug Analysis., 22, 296 (2014). https://doi.org/10.1016/j.jfda.2013.11.001
  7. Y. S. Duan, M. A. Kim, J. H. Seong, Y. G. Lee, D. S. Kim, H. S. Chung and H. S. Kim, Impacts of various solvent extracts from wild haw (Crataegus pinnatifida Bunge) pulpy on the antioxidative activities, J. East Asian Soc. Dietary Life, 24, 392 (2014).
  8. Y. S. Duan, M. A. Kim, J. H. Seong, D. S. Kim, H. S. Chung and H. S. Kim, Antioxidative activities of various solvent extracts from haw (Crataegus pinnatifida Bunge), Korean J. Food Preserv., 21, 246 (2014). https://doi.org/10.11002/kjfp.2014.21.2.246
  9. Y. S. Duan, M. A. Kim, H. S. Kim, S. H. Jang and D. S. Kang, Screening of antioxidant activity from exocarp of watermelon (Citrullus vulgaris L.), J. Environ. Sci. Int., 23, 1233 (2014). https://doi.org/10.5322/JESI.2014.23.7.1233
  10. A. A. Elzaawely, T. D. Xuan and S. Tawata, Antioxidant and Antibacterial Activities of Rumex japonicus HOUTT. Aerial Parts, Biol. Pharm. Bull., 28, 2225 (2005). https://doi.org/10.1248/bpb.28.2225
  11. T. Finkel and N. J. Holbrook, Oxidants, oxidative stress and the biology of aging, Nature, 408, 239 (2000). https://doi.org/10.1038/35041687
  12. D. Harrison, K. K. Griendling, U. Landmesser, B. Hornig and H. Drexler, Role of oxidative stress in atherosclerosis, American J. Cardiology, 91, 7 (2003). https://doi.org/10.1016/S0002-9149(02)03144-2
  13. P. C. H. Hollman, M. G. L. Hertog and M. B. Katan, Analysis and health effects of flavonoids, Food Chem., 57, 43 (1996). https://doi.org/10.1016/0308-8146(96)00065-9
  14. A. Ismail, Z. M. Marjan and C. W. Foong, Total antioxidant activity and phenolic content in selected vegetables, Food Chem., 87, 581 (2004). https://doi.org/10.1016/j.foodchem.2004.01.010
  15. R. H. Liu, Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals, American Soc. Clinical Nutr., 78, 517S (2003). https://doi.org/10.1093/ajcn/78.3.517S
  16. L. S. Lai, S. T. Chou and W. W. Chao, Studies on the antioxidative activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum. J. Agric. Food Chem., 49, 963 (2001). https://doi.org/10.1021/jf001146k
  17. L. Migliore, F. Coppede, Genetic and environmental factors in cancer and neurodegenerative diseases, Mutation Res., 512, 135 (2002). https://doi.org/10.1016/S1383-5742(02)00046-7
  18. A. M. Nuutila, R. Puupponen-Pimia, M. Aarni and K. M. Oksman-Caldentey, Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity, Food Chem., 81, 485 (2003). https://doi.org/10.1016/S0308-8146(02)00476-4
  19. R. Prior and G. H. Cao, Antioxidant phytochemicals in fruits and vegetables: diet and health implications, Hort Sci., 35, 588 (2000).
  20. F. A. Ramos, Y. Takaishi, M. Shirotori, Y. Kawaguchi, K. Tsuchiya, H. Shibata, T. Higuti, T. Tadokoro and M. Takeuchi, Antibacterial and antioxidant activities of quercetin oxidation products from yellow onion (Allium cepa) skin, J. Agric. Food Chem., 54, 3551 (2006). https://doi.org/10.1021/jf060251c
  21. V. Roginsky and E. A. Lissi, Review of methods to determine chain-breaking antioxidant activity in food, Food Chem., 92, 235 (2005). https://doi.org/10.1016/j.foodchem.2004.08.004
  22. R. J. Robbins, Phenolic acids in foods: an overview of analytical methodology, J. Agric. Food Chem., 51, 2866 (2003). https://doi.org/10.1021/jf026182t
  23. C. A. Rice-Evans, N. J. Miller and G. Paganga, Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free radical Bio. Med., 20, 933 (1996). https://doi.org/10.1016/0891-5849(95)02227-9
  24. J. Santas, R. Carbo, M. H. Gordon and M. P. Almajano, Comparison of the antioxidant activity of two Spanish onion varieties, Food Chem., 107, 1210 (2008). https://doi.org/10.1016/j.foodchem.2007.09.056
  25. D. Saleheen, S. A. Ali and M. M. Yasinzai, Antileishmanial activity of aqueous onion extract in vitro, Fitoterapia, 75, 9 (2004). https://doi.org/10.1016/j.fitote.2003.07.010
  26. H. J. Suh, J. M. Lee, J. S. Cho, Y. S. Kim and S. H. Chung, Radical scavenging compounds in onion skin, Food Res. Int., 32, 659 (1999). https://doi.org/10.1016/S0963-9969(99)00141-6
  27. B. N. Singh, B. R. Singh, R. L. Singh, D. Prakash, D. P. Singh, B. K. Sarma, G. Upadhyay and H. B. Singh, Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities, Food Chem. Toxicol., 47, 1161 (2009). https://doi.org/10.1016/j.fct.2009.02.004
  28. Y. X. Sun, S. Hayakawa, M. Ogawa and K. Izumori, Antioxidant properties of custard pudding dessert containing rare hexose, D-psicose, Food Control, 18, 220 (2005).
  29. P. Stratil, B. Klejdus and V. Kuban, Determination of Total Content of Phenolic Compounds and Their Antioxidant Activity in Vegetables-Evaluation of Spectrophotometric Methods, J. Agric. Food Chem., 54, 607 (2006). https://doi.org/10.1021/jf052334j
  30. Y. S. Velioglu, G. Mazza, L. Gao and B. D. Oomah, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products, J. Agric. Food Chem., 46, 4113 (1998). https://doi.org/10.1021/jf9801973
  31. E. A. Wilson and B. Demmig-Adams, Antioxidant, anti-inflammatory, and antimicrobial properties of garlic and onions, Nutr. Food Sci., 37, 178 (2007). https://doi.org/10.1108/00346650710749071

Cited by

  1. Quantification of flavonoids, total phenols and antioxidant properties of onion skin: a comparative study of fifteen Indian cultivars vol.57, pp.7, 2015, https://doi.org/10.1007/s13197-020-04277-w