Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Comparison of Four Multi-herbal Formulas in Antioxidant and Anticancer Activities

네 가지 처방의 항산화 및 항암 효과 비교

  • Kim, Hong Jae (Department of Pharmacy, College of Pharmacy, Pusan National University) ;
  • Choi, Eun Ok (Anti-Aging Research Center, Dong-eui University) ;
  • Kim, Min Young (Department of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • Son, Da Hee (Department of Biochemistry, Dong-eui University College of Korean Medicine) ;
  • Jeong, Jin-Woo (Anti-Aging Research Center, Dong-eui University) ;
  • Lee, Sang-hyup (Department of Korean Medical Classics, Dong-eui University College of Korean Medicine) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dong-eui University) ;
  • Choi, Yung Hyun (Anti-Aging Research Center, Dong-eui University) ;
  • Hong, Su Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine)
  • 김홍재 (부산대학교 약학대학 약학교실) ;
  • 최은옥 (동의대학교 항노화연구소) ;
  • 김민영 (부산대학교 자연과학대학 생명시스템학과) ;
  • 손다희 (동의대학교 한의과대학 생화학교실) ;
  • 정진우 (동의대학교 항노화연구소) ;
  • 이상협 (동의대학교 한의과대학 원전학교실) ;
  • 박철 (동의대학교 자연생활과학대학 분자생물학과) ;
  • 최영현 (동의대학교 항노화연구소) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실)
  • Received : 2016.10.25
  • Accepted : 2016.11.13
  • Published : 2016.11.30
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Abstract

Objective : In this study, we compared the antioxidant and anticancer properties of four multi-herbal formulas which were recorded in 'Dongeuibogam': Gilgyung-tang (GGT), Mokdanpi-tang (MDPT), Samso-eum (SSE), Samchulbobi-tang (SCBBT). Methods : We checked antioxidant properties of four multi-herbal formula through total phenolic content, radical scavenging activities, protective effects on genomic DNA oxidation. To investigate anticancer effects, we conducted MTT assay and analyzed morphologic change in A549 non-small lung cancer cells. Results : Total phenolic contents of four multi-herbal formulas were in a rich order of MDPT > SSE > GGT > SCBBT. Especially, MDPT revealed the highest activity than others in all antioxidant experiments. Our results indicated that treatment of those multi-herbal formulas induced growth retardation in A549 cells and MDPT also showed the highest anticancer effect ($IC_{50}=1.374mg/ml$) among them. Conclusions : Our data suggested that MDPT would be a powerful ingredient for lung cancer treatment.

Keywords

References

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