Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Follistatins have potential functional role in Porcine Embryogenesis

  • Kim, Dong-Hee (Division of Animal and Dairy Science, Chungnam National University) ;
  • Chun, Ju Lan (Division of Animal and Dairy Science, Chungnam National University) ;
  • Lee, Ji Hye (Division of Animal and Dairy Science, Chungnam National University) ;
  • Kim, Keun Jung (Division of Animal and Dairy Science, Chungnam National University) ;
  • Kim, Eun Young (Division of Animal and Dairy Science, Chungnam National University) ;
  • Lee, Bo Myeong (Division of Animal and Dairy Science, Chungnam National University) ;
  • Zhuang, Lili (Division of Animal and Dairy Science, Chungnam National University) ;
  • Kim, Min Kyu (Division of Animal and Dairy Science, Chungnam National University)
  • Received : 2015.10.17
  • Accepted : 2016.03.08
  • Published : 2016.03.31
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Abstract

In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-${\beta}$ superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.

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References

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