Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activities and Antimicrobial Effects of Extracts from Auricularia auricula-judae

목이버섯(Auricularia auricula-judae) 추출물의 항산화 활성 및 항균 효과

  • Yu, Sang-Cheol (Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University) ;
  • Oh, Tae-Jin (Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University)
  • 유상철 (선문대학교 BT융합제약공학과) ;
  • 오태진 (선문대학교 BT융합제약공학과)
  • Received : 2015.11.10
  • Accepted : 2016.02.17
  • Published : 2016.03.31
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Abstract

This study investigated the antioxidant and antimicrobial activities of various solvents (acetone, ethyl acetate, and ethanol) for extraction of Auricularia auricula-judae. Antioxidant activity was evaluated by determining total polyphenol and flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity. Total polyphenol and flavonoid contents were not significantly different among the extracts, whereas DPPH radical scavenging activity and ABTS cation radical scavenging activity were significantly higher in ethanol and acetone extracts. DPPH radical scavenging activities of ethanol and acetone extracts showed high values (58.7% and 46.7%, respectively). The antimicrobial properties of these extracts were determined against six bacterial pathogens (Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae) by the disc diffusion method. The acetone extracts showed antimicrobial activities against all tested bacteria, and all extracts showed the highest antimicrobial activity against B. subtilis.

본 연구는 목이버섯을 대상으로 acetone, ethanol 및 ethyl acetate 등의 용매를 이용하여 확보된 각각의 추출물에 대한 폴리페놀 함량, 플라보노이드 함량, DPPH 라디칼 소거능 및 ABTS 라디칼 소거능 등의 항산화 활성과 6종의 균주에 대한 디스크 확산법으로 항균 활성 등을 측정하였다. 목이버섯의 폴리페놀 함량과 플라보노이드 함량은 용매에 따라 유의적 차이는 없었으나 극성이 큰 acetone과 ethanol에서 ethyl acetate 추출물에 비해 다소 높게 조사되었으며, 플라보노이드 함량은 ethyl acetate> acetone> ethanol 추출물 순으로 측정되었다. DPPH 라디칼 소거능과 ABTS 양이온 라디칼 소거능은 각각 58.7~23.1%, 32.2~10.0% 등으로 나타났으며, 특히 목이버섯 ethanol 추출물에서 높은 항산화 활성을 확인하였다. 그리고 목이버섯의 항균 활성 측정 결과 acetone 추출물이 Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa 및 Enterobacter cloacae 등 6개의 균주 모두에 대하여 활성을 확인할 수 있었으며, 특히 acetone, ethanol 및 ethyl acetate 추출물 모두에서 다른 균에 비해 B. subtilis에 대하여 생육저해환이 다소 크다는 것을 알 수 있었다. 이렇듯 본 연구를 통하여 목이버섯 추출물의 항산화 및 항균 활성을 확인함으로써 향후 목이버섯을 이용한 천연 첨가제 혹은 건강 기능성 식품의 원료소재 등으로 이용 가능할 것으로 생각한다.

Keywords

References

  1. Mizuno M, Morimoto M, Minato K, Tsuchida H. 1998. Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice. Biosci Biotechnol Biochem 62: 434-437. https://doi.org/10.1271/bbb.62.434
  2. Nakajima A, Ishida T, Koga M, Takeuchi T, Mazda O, Takeuchi M. 2002. Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice. Int Immunopharmacol 2: 1205-1211. https://doi.org/10.1016/S1567-5769(02)00056-5
  3. Jang JH, Jeong SC, Kim JH, Lee YH, Ju YC, Lee JS. 2011. Characterization of a new antihypertensive angiotensin I- converting enzyme inhibitory peptide from Pleurotus cornucopiae. Food Chem 127: 412-418. https://doi.org/10.1016/j.foodchem.2011.01.010
  4. Kang MG, Bolormaa Z, Lee JS, Seo GS, Lee JS. 2011. Antihypertensive activity and anti-gout activity of mushroom Sarcodon aspratus. Kor J Mycol 39: 53-56. https://doi.org/10.4489/KJM.2011.39.1.053
  5. Kabir Y, Kimura S, Tamura T. 1998. Dietary effect of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypertensive rats (SHR). J Nutr Sci Vitaminol 34: 433-438.
  6. Kawagishi H, Furukawa S, Zhuang C, Yunoki R. 2002. The inducer of the synthesis of nerve growth factor from lion's mane (Hericium erinaceus). Explore 11: 46-51.
  7. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing. (an edible mushroom). Cancer Res 30: 2776-2781.
  8. Tsukagoshi S, Ophashi F. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use. Gann 65: 557-558.
  9. Lee J, Ahn RM, Choi HS. 1997. Determinations of ergocalciferol and cholecalciferol in mushrooms. Korean J Soc Food Sci 13: 173-178.
  10. Lee SA, Chung KS, Shim MJ, Choi EC, Kim BK. 1981. Studies on the antitumor components of Korean basidiomycetes (II): Antitumor components of Schizophyllum commune and Auricularia auricula-judae. Kor J Mycol 9: 25-29.
  11. Misaki A, Kakuta M, Sasaki T, Tanaka M, Miyaji H. 1981. Studies on interrelation of structure and antitumor effects of polysaccharides: antitumor action of periodate-modified, branched (1 goes to 3)-$\beta$-D-glucan of Auricularia auriculajudae, and other polysaccharides containing (1 goes to 3)- glycosidic linkages. Carbohydr Res 92: 115-129. https://doi.org/10.1016/S0008-6215(00)85986-8
  12. Song G, Qizhen D. 2011. Structure characterization and antitumor activity of an $\alpha$, $\beta$-glucan polysaccharide from Auricularia polytricha. Food Res Int 45: 381-387.
  13. Cheng HH, Hou WC, Lu ML. 2002. Interactions of lipid metabolism and intestinal physiology with Tremella fuciformis Berk edible mushroom in rats fed a high-cholesterol diet with or without Nebacitin. J Agric Food Chem 50: 7438- 7443. https://doi.org/10.1021/jf020648q
  14. Jo S, Kim T, Yu Y, Oh J, Jang M, Park K. 2012. A comparative study on the physiological activities of Auricularia spp. Korean J Food Sci Technol 44: 350-355. https://doi.org/10.9721/KJFST.2012.44.3.350
  15. Ko MS, Lee SJ, Kang SM. 2009. Effect of Tremella fuciformis Berk on anti-stress activities during long-term and short-term in mice. KSBB J 24: 131-139.
  16. Ham SS, Kim DH, Lee DS. 1997. Antimutagenic effects of methyl alcohol extracts from Auricularia auricula and Gyrophora esculenta. Korean J Food Sci Technol 29: 1281- 1287.
  17. Kho YS, Vikineswary S, Abdullah N, Kuppusamy UR, Oh HI. 2009. Antioxidant capacity of fresh and processed fruit bodies and mycelium of Auricularia auricula-judae (Fr.) Quel. J Med Food 12: 167-174. https://doi.org/10.1089/jmf.2007.0568
  18. Li S, Xu S, Zhang L. 2010. Advances in conformations and characterizations of fungi polysaccharides. Acta Polym Sin 12: 1359-1375.
  19. Singleton VL, Rossi JA Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158.
  20. Smith H, Doyle S, Murphy R. 2015. Filamentous fungi as a source of natural antioxidants. Food Chem 185: 389-397. https://doi.org/10.1016/j.foodchem.2015.03.134
  21. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radicals Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Piddock LJ. 1990. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J Appl Bacteriol 68: 307-318. https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  24. Di Carlo G, Mascolo N, Izzo AA, Capasso F. 1999. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci 65: 337-353. https://doi.org/10.1016/S0024-3205(99)00120-4
  25. Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E. 1997. Why drinking green tea could prevent cancer. Nature 387: 561. https://doi.org/10.1038/42381
  26. Kim T, Jo S, Kim M, Yu Y, Jang M, Park K. 2012. Comparative study on nutritional contents of Auricularia spp. J Mushroom Science and Production 10: 29-36.
  27. Metivier RP, Francis FJ, Clydesdale FM. 1980. Solvent extraction of anthocyanins from wine pomace. J Food Sci 45: 1099-1100. https://doi.org/10.1111/j.1365-2621.1980.tb07534.x
  28. Prior RL, Lazarus SA, Cao G, Muccitelli H, Hammerstone JF. 2001. Identification of procyanidins and anthocyanins in blueberries and cranberries (Vaccinium spp.) using highperformance liquid chromatography/mass spectrometry. J Agric Food Chem 49: 1270-1276. https://doi.org/10.1021/jf001211q
  29. Xu BJ, Chang SK. 2007. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 72: S159-166. https://doi.org/10.1111/j.1750-3841.2006.00260.x
  30. Ariga T, Koshiyama I, Fukushima D. 1988. Antioxidative properties of procyanidin B1 and B3 from azuki beans in aqueous systems. Agric Biol Chem 52: 2717-2722.
  31. Landrault N, Poucheret P, Ravel P, Gasc F, Cros G, Teissedre PL. 2001. Antioxidant capacities and phenolics levels of French wines from different varieties and vintages. J Agric Food Chem 49: 3341-3348. https://doi.org/10.1021/jf010128f
  32. Zhao J, Wang J, Chen Y, Agarwal R. 1999. Anti-tumorpromoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20: 1737-1745. https://doi.org/10.1093/carcin/20.9.1737
  33. Han SR, Kim MJ, Oh TJ. 2015. Antioxidant activities and antimicrobial effects of solvent extracts from Lentinus edodes. J Korean Soc Food Sci Nutr 44: 1144-1149. https://doi.org/10.3746/jkfn.2015.44.8.1144
  34. Ferreira IC, Barros L, Abreu RM. 2009. Antioxidants in wild mushrooms. Curr Med Chem 16: 1543-1560. https://doi.org/10.2174/092986709787909587
  35. Alves MJ, Ferreira ICFR, Froufe HJC, Abreu RMV, Martins A, Pintado M. 2013. Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. J Appl Microbiol 115: 346-357. https://doi.org/10.1111/jam.12196
  36. Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  37. Kim HJ, Bae JT, Lee JW, Hwang Bo MH, Im HG, Lee IS. 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts. Korean J Food Preserv 12: 80-85.
  38. Han JH, Moon HK, Chung SK, Kang WW. 2013. Comparison of antioxidant activities of radish bud (Raphanus sativus L.) according to extraction solvents and sprouting period. J Korean Soc Food Sci Nutr 42: 1767-1775. https://doi.org/10.3746/jkfn.2013.42.11.1767
  39. Shon HK, Lee YS, Park YH, Kim MJ, Lee KA. 2008. Physico- chemical properties of Gugija (Lycii fructus) extracts. Korean J Food Cookery Sci 24: 905-911.
  40. Awika JM, Rooney LW, Wu X, Prior RL, Cisneros-Zevallos L. 2003. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J Agric Food Chem 51: 6657-6662. https://doi.org/10.1021/jf034790i
  41. Jin SY. 2011. Study on antioxidant activities of extracts from different parts of Korean and Iranian pomegranates. J Korean Soc Food Sci Nutr 40: 1063-1072. https://doi.org/10.3746/jkfn.2011.40.8.1063
  42. Hong MH, Jin YJ, Pyo YH. 2012. Antioxidant properties and ubiquinone contents in different parts of several commercial mushrooms. J Korean Soc Food Sci Nutr 41: 1235- 1241. https://doi.org/10.3746/jkfn.2012.41.9.1235
  43. Song JH, Kim HS, Kim YG, Son BG, Choi YW, Kang JS. 1999. Antimicrobial activity of extract from Smilax china. J Agri Tech Dev Inst 3: 163-168.
  44. Kim JY, Lee JA, Kim KN, Song GP, Park SY. 2007. Antioxidative and antimicrobial activities of Euphorbia helioscopia extracts. J Korean Soc Food Sci Nutr 36: 1106-1112. https://doi.org/10.3746/jkfn.2007.36.9.1106

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