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Quality Characteristics of Doenjang Prepared with Fermented Hwangchil (Dendropanax morbifera) Extract

황칠 발효액을 첨가한 된장의 품질 특성

  • Received : 2015.11.17
  • Accepted : 2016.01.20
  • Published : 2016.03.31

Abstract

This study was performed to investigate the quality characteristics of various salted (8, 10, and 12%) Doenjang prepared with fermented Hwangchil (Dendropanax morbifera) extract as well as the effect of Bacillus methylotrophicus S8 strain, isolated from soy sauce, as a starter culture. After fermentation, the total cell number of low-salted Doenjang (8% and 10%) prepared with fermented Hwangchil was lower than that of Doenjang without fermented Hwangchil. Low-salted Doenjang (8% and 10%) showed higher total acidity, amino acid, and organic acid levels as well lower pH levels than high-salted Doenjang (12%), indicating active fermentation. The highest levels of lactic acid (48.1 mg/L) and glutamic acid (549.0 mg/L) were observed in low-salted Doenjang (8%) fermented with B. methylotrophicus S8 strain. The low-salted Doenjang (8%) fermented with B. methylotrophicus S8 also showed the highest score in overall preference of sensory evaluation. These results indicate that Hwangchil extract fermented with B. methylotrophicus S8 can be used as a starter for making low-salted Doenjang, resulting in improved palatability, and inhibition of abnormal fermentation.

황칠 추출물에 미생물 균주를 달리 접종하여 제조한 황칠 발효액을 스타터로 첨가하여 제조한 된장의 발효 및 품질 특성을 조사하였다. 황칠 발효액 접종 시 총균수가 log 8 CFU/mL 이상으로 스타터로서 적용 가능성을 확인하였고, 발효 후에는 염도 8%, 10%의 경우 황칠 발효액을 첨가하여 제조한 된장의 총균수가 황칠 발효액을 첨가하지 않은 된장보다 낮았다. pH의 경우 염도 8%, 10% 된장이 12% 된장에 비하여 낮은 pH 값을 보였으며, 이와 반대로 산도는 12% 된장이 낮았다. 총유리아미노산 함량은 발효 후 2,162.5~2,872.2 mg/L로 발효 전보다 함량이 증가하였고 염도가 낮아질수록 총 유리아미노산 함량은 높았으며, Bacillus methylotrophicus S8을 이용하여 제조한 저염 된장(염도 8%, 10%)의 경우 같은 염도에서 다른 균주를 이용한 된장보다 glutamic acid 함량이 유의적으로 높았다. 황칠 발효액을 첨가하여 제조한 20일 숙성한 된장의 아미노태 질소는 413.4~700.7 mg%로 염도가 낮을수록 높았으며, 특히 B. methylotrophicus S8을 이용하여 제조한 된장에서 높은 함량을 보였다. 관능평가 결과 B. methylotrophicus S8과 Saccharomyces bayanus를 접종하여 제조한 된장에서 좋은 평가를 보였으며 8% 염도의 된장에서 B. methylotrophicus S8의 전체적 기호도가 가장 높게 평가되었다.

Keywords

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