Membrane Journal (멤브레인)

The Membrane Society of Korea (한국막학회)
권호 표지
DOI QR코드

DOI QR Code

Preparation and Characterization of Porous Polycaprolactone Membrane for Tissue Engineering

조직공학용 다공성 Polycaprolactone 멤브레인의 제조 및 특성

  • Kim, Jin-Tae (R&D Center, Neobiotech co., Ltd.) ;
  • Kim, Tae-Hyung (Department of Radiological Science, Kangwon National University) ;
  • Choi, Jae Ha (Department of Advanced Material Engineering, Chungbuk National University)
  • 김진태 ((주)네오바이오텍 치과재료연구소) ;
  • 김태형 (국립강원대학교 방사선학과) ;
  • 최재하 (국립충북대학교 신소재공학과)
  • Received : 2015.11.30
  • Accepted : 2015.12.31
  • Published : 2016.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Polycaprolactone (PCL) has been fabricated into the membrane type scaffolds of 3 dimensional pore network for the tissue engineering applications by the blade method of salt (NaCl) leaching and solution casting. In this study, the experimental designs have each conditions of drying temperature, salt particle size, salt content. The modified dispensing pump connected up to homogenizing mixer system is used for mixing the $PCL/CHCl_3$ solution and NaCl particles. The membrane fabricated use by the film applicator to poured mixed solution on the glass plate. The great pore by NaCl particles and the small pore by the evaporated $CHCl_3$ in the frame wall of great pores are multiply formed in membrane scaffolds.

폴리카프로락톤(PCL)에 NaCl을 혼합한 용액을 블레이드법에 의하여 막형태로 제조한 후 NaCl을 추출하는 염출법을 이용하여 조직공학적으로 사용할 3차원 다공망을 갖는 멤브레인 형태의 지지체를 제조하였다. 본 연구에서는 성형된 멤브레인의 건조조건과, NaCl 입자의 크기, NaCl의 혼합량을 각각 다르게 하여 제조하였다. 별도로 제작한 고분자용액 공급장치를 이용하여 PCL/클로로포름($CHCl_3$) 용액에 NaCl 입자가 균일하게 혼합된 용액을 유리판에 분주하여 필름 어플리케이터를 사용하여 블레이드법에 의한 멤브레인을 제조하였다. 멤브레인 지지체에는 NaCl 입자에 의한 거대기공과 거대기공을 이루는 구조벽에서는 $CHCl_3$의 증발에 의한 미세기공이 함께 복합적으로 상호 연결되어 형성되었다.

Keywords

References

  1. K. W. Nam, T. Kimura, S. Funamoto, and A. Kishida, "Preparation of a collgen/polymer hybrid gel designed for tissue membranes. Part I: Controlling the polymer-collagen cross-linking process using an ethanol/water co-solvent", Acta Biomaterialia, 6, (2010).
  2. J. T. Kim, S. M Lim, B. S. Kim, D. Y. Lee, and J. H. Choi, "Fabrication and characterization of 3-D porous collagen scaffold", J. of Biomedical Eng. Res., 35 (2014).
  3. S. K. Hahn, J. K. Park, T. Tomimatsu, and T. Shimoboji, "Synthesis and degradation test of hyaluronic acid hydrogels", Inter. J. of Biological Macromolecules, 40 (2007).
  4. Y. Luo, K. R. Kirker, and G. D. Prestwich, "Cross-linked hyaluronic acid hydrogel films: New biomaterials for drug delivery", J. of Controlled Release, 69 (2000).
  5. J. T. Kim, C. H. Kook, and J. H. Choi, "Production equipment and method of polymer gel for bio-implantig", J. of Korean Soc. Of Mechanial Technology, 11, 3 (2009).
  6. G. H. Cho and S. I. Cheong, "Degradation characteristics of cross-linked hyaluronic acid membrane", Membr. J., 19, 4 (2009).
  7. G. S. Han, J. E. Bae, I. S. Kim, and S. I. Cheong, "Application of hyaluronic acid membrane crosslinked with 1,3-Butadiene diepoxide", Membr. J., 18, 2 (2008).
  8. H. M. Kim, J. Y. Park, E. Y. Kim, J. E. Song, S. Y. Kwon, J. W. Chung, and G. S. Khang, "Tissue engineered cartilage reconstruction with alginate sponge containing demineralized bone particles", Polymer(Korea), 38, 3 (2013).
  9. O. J. Jeon, S. J. Song, K. J. Lee, M. H. Park, S .H. Lee, S. K. Hahn, S. J. Kim, and B. S. Kim, "Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densities", Carbohydrate Polymers, 70 (2007).
  10. J. T. Kim, D. Y. Lee, E. J. Kim, J. W. Jang, and N. I. Cho, "Tissue response to implants of hyaluronic acid hydrogel prepared by microbeads", Tissue Engineering and Regenerative Medicine, 11, 1 (2014).
  11. M. X. Li, S. H. Kim, S. H. Park, J. K. Lee, and W. I. Lee, "A study on the effect of CNT on crystallization kinetics and hydrolytic degradation of PKA/CNT composite", Composites Research, 24, 4 (2011).
  12. H. Deplaine, J. L. G. Ribelles, and G. G. Ferrer, "Effect of the content of hydroxyapatite nanoparticles on the properties and bioactivity of poly(L-lactide) - Hybrid membranes", Composites Science and Technology, 70 (2010).
  13. W. Jen Lin, D. R. Flanagan, and R. J. Linhardt, "A novel fabrication of poly(${\varepsilon}$-caprolactone) and poly(ethylene glycol)s", Polymer, 40 (1999).
  14. A. G. A. Coombes, S. C. Rizzi, M. Williamson, J. E. Barralet, S. Downes, and W. A. Wallace, "Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery", Biomaterials, 25 (2004).
  15. T. Tanaka, T. Tsuchiya, H. Takahashi, M. Taniguchi, and D. R. Lloyd, "Microfiltration membrane of polymer blend of poly(L-lactic acid) and poly(${\varepsilon}$-caprolactone)", Desalination, 193 (2006).
  16. S. W. Son, J. E. Choi, D. J. Kang, D. Y. Lee, J. T. Kim, and J. W. Jang, "Synthesis and characterization of porous poly(${\varepsilon}$-caprolactone)/silica nanocomposites", Polymer(Korea), 39, 2 (2015).
  17. T. H. Yun, K. S. Kim, S. H. Cho, and K. H. Youm, "Preparation of polycaprolactone microcapsules by membrane emulsification method and its drug release properties", Membr. J., 17, 1 (2007).
  18. J. E. Jang, H. M. Kim, H. S. Kim, D. Y. Jeon, C. H. Park, S. Y. Kwon, J. W. Chung, and G. S. Khang, "Inflammatory responses to hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with variation of compositions", Polymer(Korea), 38, 2 (2013).
  19. J. H. Lee, J. R. Lee, and H. J. Kang, "Preparation of poly(lactic acid) scaffolds by the particulate leaching", J. of Korean Oil Chemists' Soc., 20, 4 (2003).
  20. Mikos A. G, A. J. Thorsen, L. A. Czerwonka, Y. Bao, R. Langer, E. N. Winslow, and J. P. Vacanti, "Preparation and characterization of poly(L-lactic acid) foams", Polymer, 35 (1994).
  21. Y. S. Nam, J. J. Yoon, and T. G. Park, "A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as porogen additive", J. of Biomed. Mater. Res., 53 (2000).
  22. Y. S. Nam and J. G Park, "Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation", J. of Biomed. Mater. Res., 47, 1 (1999). https://doi.org/10.1002/(SICI)1097-4636(199910)47:1<1::AID-JBM1>3.0.CO;2-8
  23. S. H. Lee, S. A. Park, and W. D. Kim, "Fabrication of porous 3D PCL scaffold using rapid prototyping system", Tissue Engineering and Regenerative Medicine, 7, 2 (2010).
  24. S. H. Lee, Y. S. Cho, K. P. Choi, S. H. An, and C. H. Woo, "Fabrication of PCL scaffold using autoadhesion phenomenon", J. of Korean Soc. of Mechanical Technology, 14, 2 (2012).