한국가시화정보학회지 (Journal of the Korean Society of Visualization)

  • 제14권1호
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  • Pages.51-56
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  • 2016
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  • 1598-8430(pISSN)
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  • 2093-808X(eISSN)
한국가시화정보학회 (The Korean Society of Visualization)
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VisiSens 산소 평면광 센서 시스템을 이용한 식물 잎 표면의 산소분포 가시화

Visualization of oxygen distribution on leaf surfaces using VisiSens oxygen planar optode system

  • Hwang, BaeGeun (POSTECH, Department of mechanical Engineering) ;
  • Kim, HyeJeong (POSTECH, Department of mechanical Engineering) ;
  • Lee, SangJoon (POSTECH, Department of mechanical Engineering, Biofluid and Biomimic Research Center)
  • 투고 : 2016.03.31
  • 심사 : 2016.04.22
  • 발행 : 2016.04.30
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초록

Oxygen is a key factor in aerobic reactions and most biological activities. Visualization of oxygen distribution of a chemical process or biological system has been a very challenging object despite of its significance and potential impact. To monitor and visualize the spatial distribution of oxygen concentration, various techniques such as electro-chemical probe, polarographic electrode, LIF(laser-induced fluorescence) and so on have been introduced. Oxygen planar optode which utilizes the oxygen quenching of fluorescence light is one of the currently available methods for time-resolved visualization of oxygen distribution on a planar surface. In this study, we utilized VisiSens oxygen planar optode system to visualize the spatial distribution of oxygen concentration on leaves of Korean azalea. As a result, temporal variation of oxygen concentration distribution caused by respiratory activity of the leaf could be quantitatively monitored.

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참고문헌

  1. Chen, J., Kim, H. D. and Kim, K. C., 2012, "Quantitative visualization of oxygen transfer in micro-channel using micro-LIF technique", J. Korean Soc. Vis., Vol.10(1), pp.34-39. https://doi.org/10.5407/JKSV.2011.10.1.034
  2. Lee, J. S., Kim, E. S., An, S. U., Kim, J., Kim, J. K., Khang, S. H. and Kang, D. J., 2013, "Two-dimensional oxygen distribution in a surface sediment layer measured using an RGB color ratiometric oxygen planar optode", Ocean Polar Res., Vol.35(3), pp.229-237. https://doi.org/10.4217/OPR.2013.35.3.229
  3. Glud, R. N., Ramsing, N. B., Gundersen, J. K. and Klimant, I., 1996, "Planar optrodes: a new tool for fine scale measurements of two-dimensional O2 distribution in benthic communities", Mar. Ecol. Prog. Ser., Vol.140, pp.217-226. https://doi.org/10.3354/meps140217
  4. Holst, G., Kohls, O., Klimant, I., Konig, B., Kuhl, M. and Richter, T., 1998, "A modular luminescence lifetime imgaing system for mapping oxygen distribution in biological samples. Sensor Actuat. B-Chem. Vol.51(1), pp.163-170. https://doi.org/10.1016/S0925-4005(98)00232-9
  5. Song, D. H., Kim, H. D. and Kim, K. C., 2011, "Quantitative visualization of dissolved oxygen concentration field in micro flows using PtOEP/PS membrane", J. Korean Soc. Vis., Vol.9(1), pp.36-41. https://doi.org/10.5407/JKSV.2011.9.1.036
  6. Glud, R. N., Kuhl, M., Kohls, O.and Ramsing, N. B., 1999, "Heterogeneity of oxygen production and consumption in a photosynthetic microbial mat as studied by planar optodes", J. Phycol., Vol.35(2), pp.270-279. https://doi.org/10.1046/j.1529-8817.1999.3520270.x
  7. Philip, T. W. and, Steve, R. D., 2001, "Laserinduced fluorescence studies of oxygen transfer across unsheared flat and wavy air-water interfaces", Ind. Eng. Chem. Res., Vol. 40, pp. 1985-1995. https://doi.org/10.1021/ie000321j
  8. Dani, A., Guiraud, P. and Cockx, A., 2007, "Local measurement of oxygen transfer around a single bubble by planar laser induced fluorescence", Chem. Eng. Sci., Vol.62, pp.7245-7252. https://doi.org/10.1016/j.ces.2007.08.047
  9. Francois, N., Dietrich, N., Guiraud, P., and Cockx, A., 2011, "Direct measurement of mass transfer around a single bubble by micro-PLIFI", Chem. Eng. Sci., Vol.66, pp.3328-3338. https://doi.org/10.1016/j.ces.2011.01.049
  10. Tschiersch, H., Liebsch, G., Borisjuk, L., Stangelmayer, A. and Rolletschek, H., 2012, "An imaging method for oxygen distribution, respiration and photosynthesis at a microscopic level of resolution", New Phytol., Vol.196(3), pp.926-936. https://doi.org/10.1111/j.1469-8137.2012.04295.x
  11. Zhu, F., Baker, D., Skommer, J., Sewell, M. and Wlodkowic, D., 2015, "Real-time 2D visualization of metabolic activities in zebrafish embryos using a microfluidic technology", Cytome. Part A, Vol.87(5), pp.446-450. https://doi.org/10.1002/cyto.a.22662
  12. Tschiersch, H., Borisjuk, L., Rutten, T. and Rolletschek, H., 2011, "Gradients of seed photosynthesis and its role for oxygen balancing", Biosys., Vol.103(2), pp.302-308. https://doi.org/10.1016/j.biosystems.2010.08.007
  13. Meier, J. K., Prantl, L., Muller, S., Moralis, A., Liebsch, G. and Gosau, M., 2012, "Simple, fast and reliable perfusion monitoring of microvascular flaps", Clin. Hemorh. Microcirc., Vol.50(1-2), pp.13-24.
  14. Warnat, J., Liebsch, G., Stoerr, E. M., Brawanski, A. and Woertgen, C., 2008, "Simultaneous imaging of cortical partial oxygen pressure and anatomic structures using a transparent optical sensor foil", J. Neurosurg. Anesthesiol., Vol.20(2), pp.116-123. https://doi.org/10.1097/ANA.0b013e318160545e