Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Protective Effects of Ethanol Extract of Allium hookeri Root on Acute Alcohol-Induced Intoxication in ICR Mice

급성 알코올 독성을 유발한 ICR Mouse에서 Allium hookeri 뿌리 에탄올 추출물의 간 기능 보호 효과

  • Kang, Hae-Young (Department of Food and Nutrition, Chungnam National University) ;
  • Lee, Cho-Eun (Department of Food and Nutrition, Chungnam National University) ;
  • Ly, Sun-Yung (Department of Food and Nutrition, Chungnam National University)
  • 강혜영 (충남대학교 식품영양학과) ;
  • 이초은 (충남대학교 식품영양학과) ;
  • 이선영 (충남대학교 식품영양학과)
  • Received : 2015.12.17
  • Accepted : 2016.02.20
  • Published : 2016.05.31
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Abstract

Allium hookeri is known as a healthy food since it contains larger amounts of sulfur compounds than commonly known alliaceous plants. The antioxidant and anti-inflammatory effects of A. hookeri were compared between two types of extracts, $80^{\circ}C$ water and 95% ethanol extracts of A. hookeri roots. A. hookeri root 95% ethanol extracts displayed superior total polyphenol content, antioxidant activity [1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) radical scavenging activity], and anti-inflammation activity than those of water extracts (P<0.05). We studied the effects of A. hookeri root 95% ethanol extracts (95% ethanol extracts group: AHE) on acute alcohol-induced intoxication in mice. AHE [250, 500, and 1,000 mg/kg body weight (BW)/d] was orally administered to the study group once a day for 1 week. On the last day of AHE treatment, 40% ethanol (10 mL/kg BW) was orally administered to induce acute liver injury. The blood alcohol concentration of mice treated with AHE was significantly lower compared to the control group (P<0.05). The levels of hepatic aspartate aminotransferase and alanine aminotransferase were lower in the AHE-treated group than the control group (P<0.05). The RT-PCR results for alcohol dehydrogenase and aldehyde dehydrogenase measured based on mRNA in liver tissues showed that enzyme activities were higher in the AHE-treated group than in the control group at a low blood alcohol concentration.

Allium hookeri 뿌리의 $80^{\circ}C$ 열수 추출물 및 95% 에탄올 추출물을 각각 제조하여 추출별 항산화 활성, 항염증 효과를 비교한 후 효능이 더 뛰어난 에탄올 추출물을 이용하여 급성 알코올 간 독성에 대한 보호 효과가 있는지를 살펴보았다. ICR mouse에 1주일간 A. hookeri 뿌리 추출물을 경구투여한 후 급성 알코올 간 독성을 유발하기 위하여 40% 알코올을 단회 투여한 후 혈청의 알코올 농도, AST, ALT의 변화, ADH, ALDH 활성을 측정하였다. 그 결과 A. hookeri 뿌리 추출물은 열수 추출물보다 95% 에탄올 추출물이 항산화 활성 및 항염증 효과가 더 뛰어났으며, A. hookeri 뿌리 95% 에탄올 추출물은 알코올 섭취 시 혈액 및 체내 에탄올 제거 속도를 촉진하고 혈청 AST와 ALT의 활성을 억제하며 간의 ADH, ALDH 활성을 증가시켰다. 따라서 본 연구에서는 음주로 인한 간 손상에 대해 보호 효과를 나타내는 기능성 식품 소재로서 A. hookeri 뿌리 95% 에탄올 추출물의 효능을 확인하였다.

Keywords

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