생태와환경 (Korean Journal of Ecology and Environment)

  • 제49권4호
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  • Pages.393-399
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  • 2016
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  • 2288-1115(pISSN)
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  • 2288-1123(eISSN)
한국수생태학회 (The Korean Society of Limnology)
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국내 주요 상수원지에서 독성 및 비독성 Microcystis의 분포 특성

Distribution of Toxic and Non-toxic Microcystis in Korean Water Supply

  • 이경락 (국립환경과학원 유역생태연구팀) ;
  • 신유나 (국립환경과학원 유역생태연구팀) ;
  • 이재안 (국립환경과학원 유역생태연구팀) ;
  • 이재관 (국립환경과학원 물환경연구부) ;
  • 김한순 (경북대학교 생물학과)
  • Lee, Kyung-Lak (Watershed Ecology Research Team, National Institute of Environmental Research) ;
  • Shin, Yuna (Watershed Ecology Research Team, National Institute of Environmental Research) ;
  • Lee, Jaean (Watershed Ecology Research Team, National Institute of Environmental Research) ;
  • Lee, Jae-Kwan (Water Environment Research Department, National Institute of Environmental Research) ;
  • Kim, Han Soon (Department of Biology, Kyungpook National University)
  • 투고 : 2016.11.15
  • 심사 : 2016.12.20
  • 발행 : 2016.12.31
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초록

국내 주요 상수원지 (영천호, 안계호, 가창호)에서 분리한 6종의 Microcystis (M. aeruginosa, M. ichthyoblabe, M. flos-aquae, M. novacekii, M. viridis 및 M. wesenbergii)를 대상으로 microcystin 생성유전자(mcy gene) 함유률 및 생성률을 조사하였다. 조사 결과에서 M. aeruginosa는 80%(55개 균주)가 mcy gene을 함유하고 있었으며, 그 다음으로 M. ichthyoblabe는 45% (10개 균주)의 함유율을 나타내었다. 그 외에 M. flos-aquae와 M. wesenbergii는 각각 11%(4개 균주)와 33% (1개 균주)가 microcystin 생성유전자를 포함하였다. 6종의 Microcystis에서 mcy gene의 함유율은 실제 microcystin 생성율과 거의 일치하였다. 특히, 가장 많은 균주에서 mcy gene이 확인된 M. aeruginosa는 대부분 균주들(75%)이 실제로 microcystin을 생성하는 것으로 조사되었다.

This study was conducted to investigate whether the presence of mcy gene and microcystin production are related to morphological characteristics of Korean Microcystis species. We isolated 6 different species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. flos-aquae, M. novacekii, M. viridis and M. wesenbergii) from drinking water supply dams (Yeongchun, Ankei, Gachang), and used microscopic method for morphological identification, molecular method for amplifying a partial region of mcyB gene and ELISA method for microcystin analysis. In the present study, 80% of M. aeruginosa strains contained mcy gene, followed by 45% (10 strains) of M. icthyoblabe, 33% (1 strain) of M. wesenbergii, and 11% (4 strains) of M. flos-aquae. Each percentage of mcy gene in Microcystis morphospecies was similar to that of microcystin production in Microcystis morophospecies. In conclusion, the present study shows that molecular method using mcy gene primers can be used as an indirect indicator for the monitoring of toxic cyanobacteria (Microcystis).

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참고문헌

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