KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of α-agarase from Alteromonas sp. SH-1

Alteromonas sp. SH-1균 유래의 α-agarase의 특성조사

  • Lee, Sol-Ji (Department of Bioscience, Graduate School, Silla University) ;
  • Shin, Da-Young (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Jae-Deog (Department of Green-Chemistry Convergence Engineering, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience, Graduate School, Silla University) ;
  • Lee, Sang-Hyeon (Department of Bioscience, Graduate School, Silla University)
  • 이솔지 (신라대학교 일반대학원 바이오과학과) ;
  • 신다영 (신라대학교 제약공학과) ;
  • 김재덕 (신라대학교 일반대학원 그린화학융합공학과) ;
  • 이동근 (신라대학교 일반대학원 바이오과학과) ;
  • 이상현 (신라대학교 일반대학원 바이오과학과)
  • Received : 2015.11.11
  • Accepted : 2016.06.13
  • Published : 2016.06.30
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Abstract

A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at $30^{\circ}C$ and the relative agarase activity was decreased as temperature increase from $30^{\circ}C$ and thus about 90% and 70% activities were shown at $40^{\circ}C$ and $50^{\circ}C$, respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at $20^{\circ}C$ after 1.5 hour exposure at this temperature. However, its activity was less than 60% at $30^{\circ}C$ after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are ${\alpha}$-agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.

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References

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