KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhanced Chondrogenesis by Three-dimensional Co-culture of Chondrocytes and Mesenchymal Stem Cells

연골세포와 중간엽줄기세포의 3차원 Co-culture를 통한 연골화 향상

  • Hwang, Seul-Gee (Department of Biological Engineering, Inha University) ;
  • Cha, Hyun-Myoung (Department of Biological Engineering, Inha University) ;
  • Lim, Jin-Hyuk (Department of Biological Engineering, Inha University) ;
  • Lee, Ji-Hee (Department of Biological Engineering, Inha University) ;
  • Shim, Hye-Eun (Department of Biological Engineering, Inha University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • 황슬기 (인하대학교 공과대학 생물공학과) ;
  • 차현명 (인하대학교 공과대학 생물공학과) ;
  • 임진혁 (인하대학교 공과대학 생물공학과) ;
  • 이지희 (인하대학교 공과대학 생물공학과) ;
  • 심혜은 (인하대학교 공과대학 생물공학과) ;
  • 김동일 (인하대학교 공과대학 생물공학과)
  • Received : 2016.02.24
  • Accepted : 2016.06.15
  • Published : 2016.06.30
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Abstract

Two-dimensional cultivation is typically used for cell growth, but the method reduces the characteristics of chondrocytes and stem cells, and limits culture area. Therefore, development of three-dimensional culture method is needed to mimic in vivo environment, improve quality of cells and scale-up efficiently. Improving proliferation and chondrogenesis is available by co-culture of chondrocytes and mesenchymal stem cells (MSCs) that leads to interaction between two kinds of cells. However, the co-culture has problems that permeability of sphere diminishes as aggregate size increased and ratio of two kinds of cells composing each spheres is different. In this work, co-cultivation method using controlled sphere composed of chondrocytes and MSCs was established and enhanced chondrogenesis. Periosteum-derived progenitor cells (PDPCs) that are appropriate for cell therapy source of articular cartilage were used as MSCs. Controlled spheres were formed in the hanging-drop plates and shifted for being induced chondrogenesis in 35-mm non-adhesive culture dishes at a rotation rate of 60 rpm. After inducing chondrogenesis, gene expressions related with chondrogenesis were found to be improved and it was apparent that the utilization of controlled spheres promoted chondrogenesis. As a result, available numbers of cells per unit area were increased and chondrogenic differentiation ability was improved compared to typical two-dimensional culture. This approach shows the potential in cartilage regeneration as it can provide sufficient numbers of chondrocytes.

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References

  1. Fernandes, T. G., C. A. V. Rodrigues, M. M. Diogo, and J. M. S. Cabral (2014) Stem cell bioprocessing for regenerative medicine. J. Chem. Technol. Biotechnol. 89: 34-47. https://doi.org/10.1002/jctb.4189
  2. Fernndez Vallone, V. B., M. A. Romaniuk, H. Choi, V. Labovsky, J. Otaegui, and N. A. Chasseing (2013) Mesenchymal stem cells and their use in therapy: What has been achieved? Differentiation 85: 1-10. https://doi.org/10.1016/j.diff.2012.08.004
  3. Breslin, S. and L. O'Driscoll (2013) Three-dimensional cell culture: the missing link in drug discovery. Drug Discov. Today 18: 240-249. https://doi.org/10.1016/j.drudis.2012.10.003
  4. Page, H., P. Flood, and E. G. Reynaud (2013) Three-dimensional tissue cultures: current trends and beyond. Cell Tissue Res. 352: 123-131. https://doi.org/10.1007/s00441-012-1441-5
  5. Bartosh, T. J., J. H. Ylostalo, A. Mohammadipoor, N. Bazhanov, K. Coble, K. Claypool, R. H. Lee, H. Choi, and D. J. Prockop (2010) Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc. Natl. Acad. Sci. USA 107: 13724-13729. https://doi.org/10.1073/pnas.1008117107
  6. Leijten, J. C., N. Georgi, L. Wu, C. A. van Blitterswijk, and M. Karperien (2012) Cell sources for articular cartilage repair strategies: shifting from monocultures to cocultures. Tissue Eng. Part B Rev. 19: 31-40.
  7. Frith, J. E., B. Thomson, and P. G. Genever (2009) Dynamic threedimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Eng. Part C Methods 16: 735-749.
  8. Wu, L., X. Cai, S. Zhang, M. Karperien, and Y. Lin (2013) Regeneration of articular cartilage by adipose tissue derived mesenchymal stem cells: perspectives from stem cell biology and molecular medicine. J. Cell. Physiol. 228: 938-944. https://doi.org/10.1002/jcp.24255
  9. Jeong, S. Y., D. H. Kim, J. Ha, H. J. Jin, S. Kwon, J. W. Chang, S. J. Choi, W. Oh, Y. S. Yang, and G. Kim (2013) Thrombospondin2 secreted by human umbilical cord bloodderived mesenchymal stem cells promotes chondrogenic differentiation. Stem Cells 31: 2136-2148. https://doi.org/10.1002/stem.1471
  10. Choi, Y. S., S. E. Noh, S. M. Lim, C. W. Lee, C. S. Kim, M. W. Im, M. H. Lee, and D. I. Kim (2008) Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic, osteogenic, and adipogenic differentiation. Biotechnol. Lett. 30: 593-601. https://doi.org/10.1007/s10529-007-9584-2
  11. Cha, H. M., S. M. Kim, Y. S. Choi, and D. I. Kim (2015) Scaffoldfree three-dimensional culture systems for mass production of periosteum-derived progenitor cells. J. Biosci. Bioeng. 120: 218-222. https://doi.org/10.1016/j.jbiosc.2014.12.019
  12. Acharya, C., A. Adesida, P. Zajac, M. Mumme, J. Riesle, I. Martin, and A. Barbero (2012) Enhanced chondrocyte proliferation and mesenchymal stromal cells chondrogenesis in coculture pellets mediate improved cartilage formation. J. Cell. Physiol. 227: 88-97. https://doi.org/10.1002/jcp.22706
  13. Kinney, M. A., T. A. Hookway, Y. Wang, and T. C. McDevitt (2014) Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics. Ann. Biomed. Eng. 42: 352-367. https://doi.org/10.1007/s10439-013-0953-9
  14. Meretoja, V. V., R. L. Dahlin, S. Wright, F. K. Kasper, and A. G. Mikos (2014) Articular chondrocyte redifferentiation in 3D co-cultures with mesenchymal stem cells. Tissue Eng. Part C Methods 20: 514-523. https://doi.org/10.1089/ten.tec.2013.0532