KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antiaging Effects of Musa sapientum L. (Banana) Leaf Extract

바나나 잎 추출물의 주름개선 효과

  • 유대성 (주식회사 에이씨티 기술연구소) ;
  • 장수진 (주식회사 에이씨티 기술연구소) ;
  • 박윤정 (주식회사 에이씨티 기술연구소) ;
  • 김승회 (단국대학교 자연과학대학 화학과) ;
  • 황형서 (세명대학교 한방바이오융합과학부 한방화장품과학과)
  • Received : 2016.02.11
  • Accepted : 2016.06.20
  • Published : 2016.06.30
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Abstract

To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharide-discarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLC-MS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.

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References

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