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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Separation and Characteristics of ADH and ALDH Activators in Fermented Lycii fructus Extract

발효 전후 구기자열매 추출물의 ADH 및 ALDH 활성인자 분리 및 특성

  • Choi, Ji-Hyun (Major in Biological Sciences, Faculty of Basic Sciences, College of Natural Science, Keimyung University) ;
  • Kang, Dong-Hee (Major in Biological Sciences, Faculty of Basic Sciences, College of Natural Science, Keimyung University) ;
  • Kim, Hyun-Soo (Major in Biological Sciences, Faculty of Basic Sciences, College of Natural Science, Keimyung University)
  • 최지현 (계명대학교 자연과학대학 기초과학부 생명과학전공) ;
  • 강동희 (계명대학교 자연과학대학 기초과학부 생명과학전공) ;
  • 김현수 (계명대학교 자연과학대학 기초과학부 생명과학전공)
  • Received : 2016.05.09
  • Accepted : 2016.06.27
  • Published : 2016.06.30
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Abstract

Lycii fructus has been traditionally used as a preventive and therapeutic medicine to treat enervation and diverse chronic diseases. In this study, we investigated whether fermentation of Lycii fructus extract (LE) increases the enzymatic activity of the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The fermentation of LE by Bacillus subtilis subsp. subtilis and Saccharomyces cerevisiae IFO 2376 was shown to increase the enzymatic activity of ADH and ALDH. TLC analysis of LE and fermented LE (FLE) showed that ADH and ALDH activities increased in different spots. Fraction No. 66 of LE and fraction No. 68 of FLE by Silica gel chromatography showed increased ADH activity of 129.1% and 148.9%, respectively. Fractions No. 128 of LE and FLE by Silica gel chromatography showed increased ALDH activity of 134.1% and 148.1%, respectively. The fraction No. 68 of FLE obtained by HPLC showed new peaks at $R_t$ 11.938min, $R_t$ 22.072min and $R_t$ 28.842min, indicating that ADH activity was increased. The LE and FLE fractions with the greatest increases in ADH activity peaked at the same time ($R_t$ 13min),whereas the LE and FLE fractions with the greatest increases in ALDH activity peaked at different times ($R_t$ 16.307min and $R_t$ 36.640min, respectively).

Keywords

References

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