Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Isolation and Quantitative Analysis of Cinnamtannin B-1 from Osmunda japonica Thunb

고비로부터 Cinnamtannin B-1의 분리 및 함량 분석

  • Kim, Min Suk (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Woo, Kyeong Wan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Lee, Ki Ho (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Lee, Hyun Joo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Lee, Seon Yu (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Kang, Byoung Man (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jeon, Byung Hun (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Cho, Jung Hee (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
  • 김민석 (한약진흥재단 한약자원본부) ;
  • 우경완 (한약진흥재단 한약자원본부) ;
  • 이기호 (한약진흥재단 한약자원본부) ;
  • 이현주 (한약진흥재단 한약자원본부) ;
  • 이선유 (한약진흥재단 한약자원본부) ;
  • 강병만 (한약진흥재단 한약자원본부) ;
  • 전병훈 (원광대학교 한의학과 병리학교실) ;
  • 조정희 (한약진흥재단 한약자원본부) ;
  • 조현우 (한약진흥재단 한약자원본부)
  • Received : 2016.06.13
  • Accepted : 2016.08.30
  • Published : 2016.09.30
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Abstract

In traditional Korean medicine, Osmunda japonica Thunb has been used as hemostasis and antipyretic treatment. The main compound "cinnamtannin B-1" was obtained by column chromatographic separation, and its structure was determined by spectroscopic methods, including $^1H$, $^{13}C$ NMR, and IT-TOF-ESI MS. Ash, moisture and extract content and acidinsoluble ash were monitored as identification test to establish the analytical methods. The optimum reflux extraction condition was 100% methanol extracted 30 min for 2 times. A quantitative analysis using HPLC method exhibited that the main compound at 24.7 min and its content was 0.96% in methanol extraction.

Keywords

References

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