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Morphological Features of Marine Dinoflagellates from Jangmok Harbour in Jinhae Bay, Korea: A Case of 30 Species in the Orders Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales

  • Shin, Hyeon Ho (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Kim, Eun Song (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Li, Zhun (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Youn, Joo Yeon (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Jeon, Seul Gi (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Oh, Seok Jin (Pukyung National University)
  • Received : 2016.06.13
  • Accepted : 2016.08.29
  • Published : 2016.09.30

Abstract

Most previous studies on dinoflagellates in Korean coastal areas were conducted without morphological descriptions and illustrations of the observed dinoflagellates. This indicates that the species and diversity of dinoflagellates may have been respectively misidentified and underestimated in the past, probably due to cell shrinkage, distortion and loss caused by sample fixation. This study provides information on the morphological observations of four dinoflagellate orders (Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales) from Jangmok Harbour in Jinhae Bay, Korea. The unfixed samples were collected weekly from December 2013 to February 2015. A total of 13 genera and 30 species were identified using light and scanning electron microscopy, although some samples were not clarified at the species level. Harmful dinoflagellates, Prorocentrum donghaiense, Tripos furca, Alexandrium affine, A. fundyense, Akashiwo sanguinea and Cochlodinium polykrikoides, were identified based on the morphological observations. The results also reflect the occurrence and identification of dinoflagellates that had not been previously recorded in Jangmok Harbour.

Keywords

References

  1. Ahn DK, YS Park, JG Park, JS Lee and JA Lee. 2000. Quantification of DSP toxins in the Mussels of the Jinhae Bay by fluorometric HPLC analysis and protein phosphatase inhibition assay. Algae 15:307-314.
  2. Baek SH, HH Shin, HW Choi, S Shimode, OM Hwang, KS Shin and Y-O Kim. 2011. Ecological behavior of the dinoflagellate Ceratium furca in Jangmok harbor of Jinhae Bay, Korea. J. Plankton. Res. 3:1842-1846.
  3. Balech E. 1995. The genus Alexandrium Halim (Dinoflagellata). Sherkin Island Marine Station, Cork, Ireland, 151 pp.
  4. Chang DS, IS Shin, JH Pyeun and YH Park. 1987. A study on paralytic shellfish poison of sea mussel Mytilus edulis. J. Kor. Soc. Fish. Tech. 20:293-299.
  5. Cho SY, J-S Ki and M-S Han. 2004. Morphological characteristics and molecular phylogeny of five unarmored dinoflagellates in Korean coastal waters. Algae 23:15-29.
  6. Cohen-Fernandez EJ, E Meave Del Castillo, IH Salgado-Ugarte and FF Pedroche. 2006. Contribution of external morphology in solving a species complex: The case of Prorocentrum micans, Prorocentrum gracile and Prorocentrum sigmoides (Dinoflagellata) from the Mexican Pacific Coast. Phycol. Res. 54:330-340. https://doi.org/10.1111/j.1440-1835.2006.00440.x
  7. Daubjerg N, G Hansen, J Larsen and O Moestrup. 2000. Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates. Phycologia 39:302-317. https://doi.org/10.2216/i0031-8884-39-4-302.1
  8. Dodge JD. 1975. The Prorocentrales (Dinophyceae). II. Revision of the taxonomy within the genus Prorocentrum. Bot. J. Linn. Soc. 71:103-125. https://doi.org/10.1111/j.1095-8339.1975.tb02449.x
  9. Fukuyo Y. 1985. Morphology of Protogonyaulax tamarensis (Lebour) Taylor and Protogonyaulax catenella (Whedon and Kofoid) Taylor from Japanese coastal waters. Bull. Mar. Sci. 37:529-537.
  10. Gomez F. 2012. A checklist and classification of living dinoflagellates (Dinoflagellata, Alveolata). CICIMAR Oceanides 27:65-140.
  11. Gomez F. 2013. Reinstatement of the dinoflagellate genus Tripos to replace Neoceratium, marine species of Ceratium (Dinophyceae, Aveolata). CICIMAR Oceanides 28:1-22.
  12. Gamez F, P Lopez-Garcia and D Moreira. 2011. Molecular phylogeny of dinophysoid dinoflagellates: The systematic position of Oxyphisis oxytoxoides and the Dinophysis hastata group (Dinophysales, Dinophyceae). J. Phycol. 47:393-406. https://doi.org/10.1111/j.1529-8817.2011.00964.x
  13. Gomez F, D Moreira, P Lopez-Garcia. 2010. Neoceratium gen. nov., a new genus for all marine species currently assigned to Ceratium (Dinophyceae). Protist 161:35-54. https://doi.org/10.1016/j.protis.2009.06.004
  14. Hallegraeff GM. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia 32:79-99. https://doi.org/10.2216/i0031-8884-32-2-79.1
  15. Han M-S, JK Jeon and Y-O Kim. 1992. Occurrence of dinoflagellate Alexandrium tamarense, a causative organism of paralytic shellfish poisoning in Chinhae Bay, Korea. J. Plankton Res. 14:1581-1592. https://doi.org/10.1093/plankt/14.11.1581
  16. Iwataki M, H Kawami and K Matsuoka. 2008. Cochlodinium fulvescens sp. nov. (Gymnodiniales, Dinophyceae), a new chain-forming unarmored dinoflagellate from Asian coasts. Phycol. Res. 55:231-239.
  17. John U, RW Litaker, M Montresor, S Murray, ML Brosnahan and DM Anderson. 2014. Formal Revision of the Alexandrium tamarense Species Complex (Dinophyceae) Taxonomy: The Introduction of Five Species with Emphasis on Molecular-based (rDNA) Classification. Protist 165:779-804. https://doi.org/10.1016/j.protis.2014.10.001
  18. Kim HS, SH Kim, MM Jung and JB Lee. 2013. New record of dinoflagellates around Jeju Island. J. Ecol. Environ. 36:273-291. https://doi.org/10.5141/ecoenv.2013.273
  19. Kim K-Y, Y-S Kim, C-H Hwang, C-K Lee, W-A Lim and C-H Kim. 2006. Phylogenetic analysis of dinoflagellate Gonyaulax polygramma Stein responsible for harmful algal blooms based on the partial LSU rDNA sequence data. Algae 21:283-286. https://doi.org/10.4490/ALGAE.2006.21.3.283
  20. Larsen J and O Moestrup. 1992. Potentially toxic phytoplankton 2. Genus Dinophysis (Dinophyceae). pp. 1-12. In ICES Identification Leaflets for Plankton 180. (Lindley JA ed.). ICES, Copenhagen,
  21. Leakey RJG, PH Burkhill and MA Sleigh. 1994. A comparison of fixatives for the estimation of abundance and biovolumeof marine planktonic ciliate populations. J. Plankton Res. 16:375-389. https://doi.org/10.1093/plankt/16.4.375
  22. Lee CK and W-A Lim. 2006. Variation of harmful algal blooms in Masan-Chinhae Bay. ScienceAsia 32:51-56.
  23. Lee J-S, JK Jeon, M-S Han, Y Oshima and T Yasumoto. 1992. Paralytic shellfish toxins in the mussel Mytilus edulis and dinoflagellate Alexandrium tamarense from Jinhae Bay, Korea. J. Korean Fish Soc. 25:144-150.
  24. Lee JB, DY Kim and JA Lee. 1998. Community dynamics and distribution of dinoflagellates and their cysts and Masan-Chinhae Bay, Korea. J. Korean Fish Soc. 1:283-292.
  25. Lee JY and M-S Han. 2007. Change of blooming pattern and population dynamics of phytoplankton in Masan Bay. J. Kor. Soc. of Oceanogr. 12:147-158.
  26. Lee WJ and U-J Yang. 2010. Temporal variations of Heterotrophic-and Photosynthetic dinoflagellates at a single station in Jangmok Bay in summer 2003. J. Env. Sci. Intern. 19:607-615. https://doi.org/10.5322/JES.2010.19.5.607
  27. Lu D, J Goebel, Y Qi, J Zou, X Han, Y Gao and Y Li. 2005. Morphological and genetic study of Prorocentrum donghaiense Lu from the East China Sea, and comparison with some related Prorocentrum species. Harmful Algae 4:494-505.
  28. Matsuoka K, HJ Cho and DM Jacobson. 2000. Observation of the feeding behavior and growth rates of the heterotrophic dinoflagellate Polykrikoides kofoidii (Polykrikaceae, Dinophyceae). Phycologia 39:82-86. https://doi.org/10.2216/i0031-8884-39-1-82.1
  29. Matsuoka K, M Iwataki and H Kawami. 2008. Morphology and taxonomy of chain-forming species of the genus Cochlodinium (Dinophyceae). Harmful Algae 7:261-270. https://doi.org/10.1016/j.hal.2007.12.002
  30. Matsuoka K, H Kawami, S Nagai, M Iwataki and H Takayama. 2009. Re-examination of cyst-motile relationships of Polykrikos kofoidii Chatton and Polykrikos schwartzii Butschli (Gymnodiniales, Dinophyceae). Rev. Palaeobot. Palynol. 154:79-90. https://doi.org/10.1016/j.revpalbo.2008.12.013
  31. Matsuyama Y, M Miyamoto and Y Kotani. 1999. Grazing impact of the heterotrophic dinoflagellate Polykrikos kofoidii on a bloom of Gymnodinium catenatum. Aqua. Microb. Ecol. 17:91-98. https://doi.org/10.3354/ame017091
  32. Orlova TY, MS Selina and OG Shevchenko. 2003. The morphology of cysts and motile cells of Gyrodinium instriatum (Dinophyta), a species new to the Sea of Russia. Russ. J. Mar. Biol. 29:120-122. https://doi.org/10.1023/A:1024037625075
  33. Park K-W, YS Suh and W-A Lim. 2012. Seasonal changes in phytoplankton composition in Jinhae Bay, 2011. J. Korean Soc. Mar. Environ. Saf. 18:520-529. https://doi.org/10.7837/kosomes.2012.18.6.520
  34. Park T-G, YS Kang and Y-T Park. 2009. Abundance of the toxic dinoflagellate Alexandrium catenella in Jinhae Bay, Korea as measured by specific real-time PCR probe. J. Korean Fish Soc. 12:227-235.
  35. Saldarriaga JF, FJR Taylor, PJ Keeling and T Cavalier-Smith. 2001. Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements. J. Mol. Evol. 53:204-213. https://doi.org/10.1007/s002390010210
  36. Shin HH, YH Yoon, H Kawami, M Iwataki and K Matsuoka. 2008. The first appearance of toxic dinoflagellate Alexandrium tamarense (Gonyaulacales, Dinophyceae) responsible for the PSP contamination in Gamak Bay, Korea. Algae 23:251-255. https://doi.org/10.4490/ALGAE.2008.23.4.251
  37. Stoecker DK, DE Gustafson and PG Verity. 1996. Micro- and mesoprotozooplankton at 140$^{\circ}W$ in the equatorial Pacific: heterotrophs and mixotrophs. Aquat. Microb. Ecol. 10:273-282. https://doi.org/10.3354/ame010273
  38. Stoecker DK, A Taniguchi and AE Michaels. 1989. Abundance of autotrophic, mixotrophic and heterotrophic ciliates in shelf and slope waters. Mar. Ecol. Prog. Ser. 50:241-254. https://doi.org/10.3354/meps050241
  39. Yoo K-I and J-B Lee. 1987. On the trophic correlation between Tintinnids and dinoflagellates in Masan Bay, Korea. Bull. Korean Fish. Soc. 20:230-236.
  40. Yoo KW. 1982. Taxonomic study on the causative organisms of red tide: Genus Prorocentrum. Bull. Environ. Sci. Hanyang Univ. 3:25-31.
  41. Yoshida M, K Mizushima and K Matsuoka. 2003. Alexandrium acatenella (Gonyaulacales: Dinophyceae): Morphological characteristics of vegetative cell and resting cyst. Plankton Biol. Ecol. 50:61-64.