Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Screening of Functional Compound of Abeliophyllum distichum Nakai for Optimize Extraction Condition and Efficacy Evaluation

미선나무의 추출조건 확립 및 효능평가를 통한 기능성 탐색

  • Cho, Seok-Cheol (Department of Food Science & Engineering, Seowon University) ;
  • Kim, Myong-Ki (Department of Food Science & Engineering, Seowon University)
  • Received : 2017.11.24
  • Accepted : 2017.12.20
  • Published : 2017.12.31
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Abstract

To evaluate optimum conditions of extract active components, extraction of Abeliophyllum distichum Nakai was performed with various ethanol and water mixture at ethanol concentration of 0, 20, 40, 60, 80 and 100%(v/v) and extraction methods. Higher polyphenol and flavonoids contents of extract achieved at 2 hours of reflux extraction in 80% ethanol. The highest DPPH free radical scavenging activity of the stem extracts by soaking method and butanol fractions of leaf extracts are 71.3% and 103.3% respectively. The results suggested that Abeliophyllum distichum Nakai can be used for valuable natural resource.

미선나무 잎과 줄기 중 유효성분의 최적 추출조건을 평가하기 위해 다양한 비율의 물과 에탄올 혼합물(0, 20, 40, 60, 80, 100% ethanol(v/v))을 추출용매로 사용하여 환류냉각, 초음파 및 침지법을 적용하여 추출을 진행하였다. 추출물의 총 폴리페놀과 플라보노이드 함량을 분석한 결과 80% 에탄올로 2시간 동안 환류냉각 추출하였을 때 가장 높은 값을 나타내었으며 미선나무 줄기의 DPPH free radical 소거능을 평가한 결과 침지 추출 시 71.3%로 가장 높았다. 미선나무 잎의 DPPH free radical 소거능은 용매별로 분획하여 측정한 결과 용매별로 다른 값을 나타내었으며 부탄올 층에서 103.3%로 가장 높게 나타났다. 이러한 결과로써 미선나무의 잎과 줄기를 생리활성을 보유한 천연물 소재로써 활용이 가능하다는 것을 확인하였다.

Keywords

References

  1. N. N. Lee, Y. E. Choi & H. K. Moon. (2014). Effect of leds on shoot multiplication and rooting of rare plant Abeliophyllum distichum Nakai. Journal of Plant Biotechnology, 41(2), 94-99. DOI : 10.5010/JPB.2014.41.2.94
  2. S. P. Hong & M. J. Han. (2002). The floral dimorphism in the rare endemic plant, Abeliophyllum distichum Nakai (Oleaceae). Flora-Morphology, Distribution, Functional Ecology of Plants, 197, 317-325. DOI : 10.1078/0367-2530-00047
  3. J. H. Park. (2011). Antioxidant Activities and Inhibitory Effect on Oxidative DNA Damage of extracts from Abeliophylli distichi Folium. Korean Journal of Herbology, 26(4), 95-99.
  4. S. B. Kwon, H. J. Kang, M. J. Kim, J. H. Kim, H. S. Shin & K. S. Kim. (2014). Analysis on the components and safety evaluation of Abeliophyllum distichum Nakai leaves and stems. Korean Journal of Environmental Health, 40(3), 234-244. DOI : 10.5668/JEHS.2014.40.3.234
  5. Kipris. (2010). Cosmetic Composition Comprising the Extract of Abelliophyllum distichum as active Ingredient (NO. 100954695). Daejeon : Kipris.
  6. Kipris. (2007). Anti-cancer composiotion comprising extract from Abeliophyllum distichum. NO. 100706131. Daejeon : Kipris.
  7. Kipris. (2017). Development of skin-care products based on antioxidative effect of Abeliophyllum distichum Nakai (NO. 101729209). Daejeon : Kipris.
  8. Y. G. Kim. (2004). Antioxidant. Seoul : Ryo Moom Gak.
  9. S. Y. Choe & K. H. Yang. (1982). Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean Journal of Food Science and Technology, 14(3), 283-288.
  10. N. Y. Kim & H. Y. Lee. (2015). Enhancement of Anti-wrinkle Activities of Abeliophyllum distichum Nakai through Low Temperature Extraction Process. Korean Journal of Medicinal Crop Science, 23(3), 231-236. DOI : 10.7783/KJMCS.2015.23.3.231
  11. Y. Hirobumi, Y. Katsuhiko, Y. Yoshida &, I. K. Kenichiro. (1998). Production of cornoside in Abeliophyllum distichum cell suspension cultures. Phytochemistry, 48(2), 273-277. DOI : 10.1016/S0031-9422(97)01134-5
  12. K. J. Lee & B. H. Um. (2008). Extraction of useful component from natural plants using ultrasound system. Korean Journal of Biotechnology and Bioengineering, 23(2), 101-108.
  13. M. D. Esclapez, J. V. Garcia-Perez, A. Mulet & J. A. Carcel. (2011). Ultrasound-assisted extraction of natural products. Food Engineering Reviews, 3(2), 108-120. DOI : 10.1007/s12393-011-9036-6
  14. Y. Ma, J. C. Chen, D. H. Liu & X. Q. Ye. (2009). Simultaneous extraction of phenolic compounds of citrus peel extracts : effect of ultrasound. Ultrasonics Sonochemistry, 16(1), 57-62. DOI : 10.1016/j.ultsonch.2008.04.012
  15. B. Karki et al. (2010). Enhancing protein and sugar release from defatted soy flakes using ultrasound technology. Journal of Food Engineering, 96(2), 270-278. DOI : 10.1016/j.jfoodeng.2009.07.023
  16. O. Folin & V. Ciocalteu. (1927). On tyrosine and tryptophane determination in proteins. Journal of Biological Chemistry, 27, 627-650.
  17. J. Zhishen, T. Mengcheng & W. Jianming. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559. DOI : 10.1016/s0308-8146(98)00102-2