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Effect of Acer tegmentosum Maxim. extract on differentiation of osteoblastic Primary calvarial osteoblasts cells

조골세포의 분화에 산겨릅나무 추출물이 미치는 영향

  • Oh, Tae Woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Shim, Ki-Shuk (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Kwang-Youn (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Cho, Won-Kyung (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang Il (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Ma, Jin Yeul (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 심기석 (한국한의학연구원 한의기술응용센터) ;
  • 김광연 (한국한의학연구원 한의기술응용센터) ;
  • 조원경 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (한국한의학연구원 한의기술응용센터) ;
  • 마진열 (한국한의학연구원 한의기술응용센터)
  • Received : 2017.11.08
  • Accepted : 2017.11.16
  • Published : 2017.11.30

Abstract

Objectives : The present study, to confirm the osteoblast differentiation effects of Acer tegmentosum Maxim. (AT) extract. Methods : In this experiment, cell viability, Alizarin red S assay, and Alkaline phosphatase (ALP) activity with AT extract (50, $100{\mu}g/m{\ell}$). Also, we studied the expression of differentiation regulator with AT extract in primary calvarial osteoblasts cells (pOB). Results : As a result of AT treatment, we determined that AT extract stimulates ALP activity and alizarin red activities in the pOB cells for mineralization for 18 days. Moreover, these factors increasing osteogenic markers such as Runt-related transcription factor2 ($Run{\times}2$), osteocalcin (OC), osteopontin, osterix, smad1, smad5, activating transcription factor4 (ATF4) and collagen type I alpha 1. Conclusions : These results indicate that AT extract have effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of bone diseases.

Acknowledgement

Supported by : 한국임업진흥원, 한국한의학연구원

References

  1. Takayanagi H., Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems., Nat Rew immunol. 2007 ; 7(4): 292-304. https://doi.org/10.1038/nri2062
  2. Katagiri, T. and N. Takahashi. Regulatory mechanisms of osteoblast and osteoclast differentiation. Oral Diseases. 2002 ; 8 : 147-59. https://doi.org/10.1034/j.1601-0825.2002.01829.x
  3. Dragsted, L. O., M. Strube, and J. C. Larsen. Cancer-protective factor in fruits and vegetables. Biocheminal and biological background. Pharmacol. Toxicol. 1993 ; 72: 116-35.
  4. Newmark, H. L. Plant phemolics as potential cancer prevention agents. Adv. Exp. Med. Biol. 1996 ; 401 : 25-34.
  5. Manolagas, S. C. Basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Birth and death of bone cells. Endocr. Rev. 2000 ; 21 : 115-37.
  6. Mone, Z. Skeletal remodeling in health and disease. Nat. Med. 2007. ; 13 : 791-801. https://doi.org/10.1038/nm1593
  7. Kang SK, Park YB, Ahn HS. The bibliographical studies in the acupuncture treatment of the osteoporosis. J Korean Acupun & Moxibu Medicin Soc. 1998 ; 15(2) : 437-54.
  8. Cho, H.S. Research on menorralgia in the questionnaire of oriental gynecology. Unpublished master's thesis. Dong-Eui University, 2002.
  9. Lee GB. Korean Plant Illustrations. Hang Mun-sa. Seoul. 1993 ; 552.
  10. So BG. Chinese main book illustrations. 3 vol. Yeogangchulpan-sa, Seoul. 1994 ; 193.
  11. Cho EK, Jung KI and Young Ju Choi. Anti-Diabetic, Alcohol Metabolizing Enzyme, and Hepatoprotective Activity of Acer tegmentosum Maxim. Stem Extracts. J Korean Soc Food Sci Nutr. 2015 ; 44(12) : 1785-92 https://doi.org/10.3746/jkfn.2015.44.12.1785
  12. Lee CE, Jeong HH, Cho IA and Sun Yung Ly. In Vitro and In Vivo Anti-Oxidative and Anti-Inflammatory Activities of Acer tegmentosum Maxim Extracts. J Korean Soc Food Sci Nutr. 2017 ; 46(1) : 1-9. https://doi.org/10.3746/jkfn.2017.46.1.001
  13. Seo YH, Lee SH, Hwang HS and Choi SY. Effects of Non-Alcoholic Fatty Liver in Rats by Acer tagmentosum Maxim. Extract. Korean J. Food & Nutr. 2016 ; 29(3) : 307-12. https://doi.org/10.9799/ksfan.2016.29.3.307
  14. Park KM, Yang MC, Lee KH, Kim KR, Choi SU, Lee KR. Cytotoxic phenolic constituents of Acer tegmentosum maxim. Arch Pharm Res. 2006 ; 29 : 1086-90. https://doi.org/10.1007/BF02969296
  15. Lee, J. W. and I. S. Lee. Effects of Rubus coreanus miquel extracts on the activity and differentiation of MC3T3-E1 osteoblastic cell. J. Life Sci. 2004 ; 14 : 967-74. https://doi.org/10.5352/JLS.2004.14.6.967
  16. Mok, S. K. and H. S. Shin. The effects of prostaglandine and dibytyryl cAMP on osteoblasic cell activity and osteoclast generation. J. Wonkwang Dental Res. Ins. 1996 ; 6 : 43-62.
  17. Solt, D. B. The pathogenesis, oral manifestations, and implications for dentistry of metabolic bone disease. Curr. Opin. Dent. 1991 ; 1 : 783-91.
  18. Parfitt, A. M. Osteonal and hemi-osteonal remodeling: The spatial and temporal framework for signal traffic in adult human bone. J. Cell Biochem. 1994 ; 55 : 273-86. https://doi.org/10.1002/jcb.240550303
  19. Bureau of Statistics. The statistics of mortality factors, Meta Data Base. 2006 ; 9
  20. Tung NH , Yan D , Kim SK , Bae KH , Kim YH. Total peroxyl radicalscavenging capacity of the chemical components from the stems of Acer tegmentosum Maxim , J Agric Food Chem, 2008 ; 56 : 10510-4. https://doi.org/10.1021/jf8020283
  21. Park KM, Yang MC, Lee KH, Kim KR, Choi SU, Lee KR. Cytotoxic phenolic constituents of Acer tegmentosum maxim. Arch Pharm Res. 2006 ; 29(12) : 1086-90. https://doi.org/10.1007/BF02969296
  22. Yu T, Lee J, Lee YG, Byeon SE, Kim MH, Sohn EH, Lee YJ, Lee SG, Cho JY. In vitro and in vivo anti-inflammatory effects of ethanol extract from Acer tegmentosum. J Ethnopharmacol. 2010 ; 128(1) : 139-47. https://doi.org/10.1016/j.jep.2009.12.042
  23. Kim EC, Kim SH, Piao SJ, Kim TJ, Bae K, Kim HS, Hong SS, Lee BI, Nam M. Antiangiogenic Activity of Acer tegmentosum Maxim Water Extract in Vitro and in Vivo. J Korean Med Sci. 2015 ; 30(7) : 979-87. https://doi.org/10.3346/jkms.2015.30.7.979
  24. Ha H, Shim KS, Kim T, An H, Lee CJ, Lee KJ, Ma JY. Water extract of Acer tegmentosum reduces bone destruction by inhibiting osteoclast differentiation and function. Molecules. 2014 ; 19(4): 3940-54. https://doi.org/10.3390/molecules19043940
  25. Shin, I.C., J.H. Sa, T.H. Shim and J.H. Lee. The physical and chemical properties and cytotoxic effects of Acer tegmentosum Maxim. extracts. J. Korean Soc. Appl. Biol. Chem. 2006 ; 49 : 322-7.
  26. Jang, S.I., H.O. Pae, B.M. Choi, G.H. Oh, S. Jeong, H.J. Lee, H.Y. Kim, K.H. Kang, Y.G. Yun, Y.C. Kim and H.T. Chung. Salidroside from Rhodiola sachalinesis protects neuronal PC 12 cells against cytotoxicity induced by amyloid-${\beta}$. Immunopharmacol. Immunotoxicol. 2003 ; 25 : 295- 304. https://doi.org/10.1081/IPH-120024498
  27. Zhang, Y. and Y. Liu. Study on effect of salidroside on lipid peroxidation in oxidative stress in rat hepatic stellate cells. Zhong Yao Cai. 2005 ; 28 : 794-6.
  28. Soltanoff CS, Yang S, Chen W, Li YP. Signaling networks that control the lineage commitment and differentiation of bone cells. Crit Rev Eukaryot Gene Expr. 2009 ; 19 :1-46. https://doi.org/10.1615/CritRevEukarGeneExpr.v19.i1.10
  29. Lee MH, Kwon TG, Park HS, Wozney JM, Kim HJ, Ryoo HM: BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2. Biochem Biophys Res Commun. 2003 ; 309(3) : 689-94. https://doi.org/10.1016/j.bbrc.2003.08.058
  30. Roca H, Phimphilai M, Gopalakrishnan R, Xiao G, Franceschi RT: Cooperative interactions between RUNX2 and homeodomain protein-binding sites are critical for the osteoblast-specific expression of the bone sialoprotein gene. J Biol Chem. 2005 ; 280 : 30845-55. https://doi.org/10.1074/jbc.M503942200
  31. Kim YJ, Lee MH, Wozney JM, Cho JY, Ryoo HM: Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2. J Biol Chem. 2004 ; 279 : 50773-80. https://doi.org/10.1074/jbc.M404145200
  32. Xiao ZS, Hinson TK, Quarles LD: Cbfa1 isoform overexpression upregulates osteocalcin gene expression in non- osteoblastic and pre-osteoblastic cells. J Cell Biochem. 1999 ; 74 : 596-605. https://doi.org/10.1002/(SICI)1097-4644(19990915)74:4<596::AID-JCB9>3.0.CO;2-F
  33. Harding HP, Zhang Y, Zeng H, NovoaI, Lu PD, Calfon M, Sadri N, Yun C, Popko B, Paules R, Stojdl DF, Bell JC, Hettmann T, Leiden JM, Ron D. An integrated stress response regulates amino acid metabolism and resistance to oxidative stress. Mol Cell,. 2003 ; 11 : 619-33. https://doi.org/10.1016/S1097-2765(03)00105-9
  34. Yang X, Matsuda K, Bialek P, Jacquot S, Masuoka HC, Schinke T, Li L, Brancorsini S, Sassone-Corsi P, Townes TM, Hanauer A, Karsenty G. ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology; implication for Coffin-Lowry Syndrome. Cell. 2004 ; 117 : 387-98. https://doi.org/10.1016/S0092-8674(04)00344-7
  35. Jiang D, Franceschi RT, Boules H, Xiao G. Parathyroid hormone induction of the osteocalcin gene. Requirement for an osteoblast-specific element 1 sequence in the promoter and involvement of multiple-signaling pathways. J Biol Chem. 2004 ; 279 : 5329-37. https://doi.org/10.1074/jbc.M311547200