대한약침학회지 (Journal of Pharmacopuncture)

  • 제20권4호
  • /
  • Pages.265-273
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  • 2017
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  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
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Orthosiphon pallidus, a Potential Treatment for Patients with Breast Cancer

  • Singh, Mukesh K. (Shri Rawatpura Sarkar Institute of Pharmacy) ;
  • Dhongade, Hemant (Shri Rawatpura Sarkar Institute of Pharmacy) ;
  • Tripathi, Dulal Krishna (Rungta College of Pharmaceutical Sciences and Research)
  • 투고 : 2017.04.14
  • 심사 : 2017.06.20
  • 발행 : 2017.12.31
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초록

Objective: Orthosiphon pallidus (O. pallidus), which belongs to the Lamiaceae family, is a popular garden plant that is widely used for the treatment of various diseases, such as urinary lithiasis, fever, hepatitis, cancer and jaundice. The objective of the present work was to investigate the antioxidant free-radical scavenging and the anticancer activities of O. pallidus against human breast-cancer cell lines. Methods: The antioxidant activity of Orthosiphon pallidus aqueous extract (OPAE) was investigated using different models, such as the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the 2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) models, as were the $Fe^+$ chelation, the hydroxyl radical and superoxide radical scavenging, and total reducing power activities. The anticancer activities of the extract were determined by using the 3-(4, 5- dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) and the sulforhodamine (SRB) assays on the MCF-7 and the MDA-MB-231 cancer cell lines. Results: The aqueous Orthosiphon pallidus extract showed potent activity in in-vitro models. It significantly inhibited the scavenging of hydroxyl and superoxide radicals, but induced a remarkable $Fe^+$ chelation activity. For both cell lines, the percent cytotoxicity was found to increase steadily with increasing OPAE concentration up to $240{\mu}g/mL$. Conclusion: These results suggest that Orthosiphon pallidus has excellent antioxidant, antimicrobial, and anticancer activities against human breast-cancer cell lines.

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참고문헌

  1. Khan WU, Khan RA, Ahmed M, Khan LU, Khan MW. Pharmacological evaluation of methanolic extract of Cyperus scariosus. Bangladesh J Pharmacol. 2016; 11: 353-8. https://doi.org/10.3329/bjp.v11i2.23611
  2. Sellem I, Kaaniche F, Mtibaa AC, Mellouli L. Anti-oxidant, antimicrobial and anti- acetylcholinesterase activities of organic extracts from aerial parts of three Tunisian plants and correlation with polyphenols and flavonoids contents. Bangladesh J Pharmacol. 2016; 11: 531-44. https://doi.org/10.3329/bjp.v11i2.26001
  3. Ameyaw Y, Duker-Eshun G. The alkaloid contents of the ethno -plant organs of three antimalarial medicinal plant species in the Eastern region of Ghana. Int J Chem Sci. 2009; 7: 48-58.
  4. Kusuma IW, Murdiyanto, Arung ET, Syafrizal , Kim Y. Antimicrobial and antioxidant properties of medicinal plants used by the Bentian tribe from Indonesia. Food Science and Human Wellness. 2014; 3:191–6. https://doi.org/10.1016/j.fshw.2014.12.004
  5. Ghaffari H, Venkataramana M, Ghassam JB., Nayaka CS., Nataraju A, Geetha NP et al. Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells. Life Sci. 2014; 113: 7-13. https://doi.org/10.1016/j.lfs.2014.07.010
  6. Dhayalan M, Jegadeeshwari AL., Gandhi NN. Biological activity sources from traditionally used tribe and herbal plants material. Asian J Pharm Clin Res. 2015; 8 (6): 11-23.
  7. George E, Kasipandi M, Venkataramana M, Naveenkumar K, Allen JA, Parimelazhagan T et al. In vitro antioxidant and cytotoxic analysis of Pogostemon mollis Benth. Bangladesh J Pharmacol. 2016; 11: 148-58. https://doi.org/10.3329/bjp.v11i1.24157
  8. Ashokan K and Muthuraman MS. Anticancer studies on Orthosiphon pallidus royle and Peristrophe bicalyculata nees. J. Pharm. Res. 2011; 4: 2654-6.
  9. Regina KMM, Adama H, Jeanne M and Odile N. Ethnobotany and Ethnopharmacognosy of Lamiaceae Species from Central Burkina Faso: Leucas martinicensis (Jacquin) R. Brown, Hoslundia opposita Vahl and Orthosiphon pallidus Royle Ex Benth. American Journal of Ethnomedicine. 2015; 2(4):219-232.
  10. Prathapan A, Lijo CO, Nampoothiri SV, Mini S and Raghu KG. In vitro antiperoxidative, free radical scavenging and xanthine oxidase inhibitory potentials of ethyl acetate fraction of Saraca ashoka flowers. Nat Prod Res. 2011a; 25 (3): 298-309. https://doi.org/10.1080/14786419.2010.510472
  11. Prathapan A, Singh MK, Anusree SS, Soban Kumar DR, Sundaresan A, Raghu KG. Antiperoxidative, free radical scavenging and metal chelating activities of Boerhaavia diffusa L. J Food Biochem. 2011b; 35: 1548-1554. https://doi.org/10.1111/j.1745-4514.2010.00477.x
  12. Al-Tahtawy RHM, El-Bastawesy AM, Monem MGA, Zekry ZK, Al-Mehdar HA, El-Merzabani MM. Antioxidant activity of the volatile oils of Zingiber Officinale (ginger). Spatula DD 2011; 1(1):1-8. https://doi.org/10.5455/spatula.20101209111419
  13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-evans C. Antioxidant activity applying on improve ABTS radical cation decolorization assay. Free Rad Bio Med 1999; 26:1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  14. Han W, Li X. Antioxidant activity of aloeswood tea in vitro. Spatula DD 2012; 2(1):43- 50. https://doi.org/10.5455/spatula.20120331054309
  15. Klein SM, Cohen G, Cederaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochem 1981; 20: 6006-12. https://doi.org/10.1021/bi00524a013
  16. Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complemen Altern Med. 2008; 8: 63. https://doi.org/10.1186/1472-6882-8-63
  17. Basniwal PK, Suthar M, Rathore GS, Gupta R, Kumar V, Pareek A, et al. In vitro antioxidant activity of hot aqueous extract of Helicteresisora Linn. Fruits. Nat prod radiance 2009; 8(5): 483-7.
  18. Thambiraj J, Paulsamy S, Sevukaperumal R. Evaluation of In- vitro antioxidant activity in the traditional medicinal shrub of western districts of Tamilnadu, India, Acalypha fruticosa Forssk. (Euphorbiaceae). Asian Pac J Trop Biomedicine. 2012; 2(1): 127-130. https://doi.org/10.1016/S2221-1691(12)60142-7
  19. Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharmaceutical Sci. 2011; 3(3): 126-128.
  20. Makari HK, Haraprasad N, Patil H, Ravikumar S. In vitro antioxidant activity of the hexane and methanolic extracts of Cordia wallichii and Celastruspaniculata. Internet J Aesthetic Antiaging Med 2008; 1: 1-10.
  21. Rahman S, Salehin F and Iqbal A. In vitro antioxidant and anticancer activity of young Zingiber officinale against human breast carcinoma cell lines. BMC Complementary and Altern Med. 2011; 11:76 https://doi.org/10.1186/1472-6882-11-76
  22. Sriwiriyajan S, Ninpesh T, Sukpondma Y, Nasomyon T, Graidist P. Cytotoxicity screening of plants of genus Piper in breast cancer cell lines. Trop. J. Pharm. Res. 2014; 13, 921-8. https://doi.org/10.4314/tjpr.v13i6.14
  23. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, et al. New Colorimetric Cytotoxicity Assay for Anticancer-drug Screening. J. Natl. Cancer Inst. 1990; 82: 1107-1112. https://doi.org/10.1093/jnci/82.13.1107
  24. Rubalakshmi G, Karmegam N. Antioxidant and anticancer activities of an endemic medicinal plant Curcuma Neilgherrensis Wt. (Zingiberaceae). IJESET 2011, 1(1): 27- 36
  25. Bhalodia NR, Nariya PB, Acharya RN, Shukla VJ. Evaluation of in vitro antioxidant activity of flowers of Cassia fistula Linn. Int J Pharm Tech Res. 2011; 3(1): 589-599.
  26. Khan A, Khan RA, Ahmed M, Mushtaq N. In vitro antioxidant, antifungal and cytotoxic activity of methanolic extract of Calligonum polygonoides. Bangladesh J Pharmacol. 2015; 10: 316-320. https://doi.org/10.3329/bjp.v10i2.22448
  27. Zhu YZ, Huang SH, Tan BK, Sun J, Whiteman M, Zhu YC. Anti-oxidants in Chinese herbal medicines: A biochemical perspective. Nat Prod Rep. 2004; 21: 479-89.
  28. Costamagna MS, Ordonez RM, Zampini IC, Sayago JE, Isla MI. Nutritional and anti- oxidant properties of Geoffroea decorticans, an Argentinean fruit, and derived products (flour, arrope, decoction and hydroalcoholic beverage). Food Res Inter. 2013; 54: 160-68. https://doi.org/10.1016/j.foodres.2013.05.038
  29. Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007; 100: 1409-18. https://doi.org/10.1016/j.foodchem.2005.11.032
  30. Thambiraj J, Paulsamy S, Sevukaperumal R. Evaluation of in vitro antioxidant activity in the traditional medicinal shrub of western districts of Tamilnadu, India, Acalypha fruticosa Forssk. (Euphorbiaceae). Asian Pac J Trop Biomedicine. 2012; 2(1): 127-130. https://doi.org/10.1016/S2221-1691(12)60142-7
  31. Kilani S, Sghaier MB, Limem I, Bouhlel I, Boubaker J, Bhouri W, et al. In vitro evaluation of antibacterial, anti-oxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperus rotundus. Bioresour Technol. 2008; 99: 9004-8. https://doi.org/10.1016/j.biortech.2008.04.066
  32. Kowalczyk A, Biskup I, Fecka I. Total phenolic content and antioxidative properties of commercial tinctures obtained from some Lamiaceae plants. Nat Prod Commun. 2012; 7: 1631-4.
  33. Ertas A, Boga M, Yesil Y. Phytochemical profile and ABTS cation scavenging, cupric reducing anti-oxidant capacity and anticholinesterase activities of endemic Ballota nigra L. subsp. anatolica P.H. Davis from Turkey. J Coast Life Med. 2014; 2: 555-9.
  34. Karima L, Mohamed T, Soufiane T. Evaluation of net antioxidant activity of mono- and bis-Mannich base hydrochlorides and 3-keto-1,5-bisphosphonates from their ProAntidex parameter. J Mol Struct. 2015; 1091: 152–8. https://doi.org/10.1016/j.molstruc.2015.02.071
  35. Asirvatham R, Christina AJM, Murali A. In Vitro Antioxidant and Anticancer Activity Studies on Drosera Indica L. (Droseraceae). Advanced Pharmaceutical Bulletin. 2013; 3(1): 115-120
  36. Fridovich I. The biology of oxygen radicals. Science. 1978; 201: 875–880. https://doi.org/10.1126/science.210504
  37. Oktay M, Gulcin I, Kufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT Food Science and Technology. 2003; 36: 263-71. https://doi.org/10.1016/S0023-6438(02)00226-8
  38. Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grapes (Vitis vinifera L.) extracts. Food Control. 2004; 5: 333-5.
  39. Akhir NAM, Chua LS, Majid FAAM, Sarmidi MR. Cytotoxicity of ethanolic extracts of Ficus deltoidea on human ovarian carcinoma cell line. Br J Med Med Res. 2011; 1: 397-409. https://doi.org/10.9734/BJMMR/2011/507
  40. Priya V, Rao S. Evaluation of anticancer activity of tridax procumbens leaf extracts on A549 and Hep G2 Cell Lines. Asian J Pharm Clin Res. 2015; 8(3) 129-132.