Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant and anti-aging activities of water extracts from Chionanthus retusus flesh according to different extraction temperatures

추출온도에 따른 이팝나무 과육 물 추출물의 항산화 및 항노화 활성

  • Choi, Hae-Sim (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Yang-Suk (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Seo, Su-Jeong (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University)
  • 최해심 (대구한의대학교 한의과대학 한의학과) ;
  • 이양숙 (대구한의대학교 한약개발학전공) ;
  • 서수정 (대구한의대학교 한약개발학전공) ;
  • 김남우 (대구한의대학교 한약개발학전공)
  • Received : 2017.11.09
  • Accepted : 2017.12.20
  • Published : 2017.12.30
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Abstract

The fringe tree, Chionanthus retusus (Oleaceae) has been used as a natural remedy that helps a paralytic stroke, dementia, phlegm and malaria. This study was conducted to investigate the antioxidant and anti-aging activities of water extracts obtained from the flesh of C. retusus by reflux extraction at room temperature and $80^{\circ}C$, and pressure heating extraction at $110^{\circ}C$, for an increment of their practical use. The highest extraction yield from the flesh of C. retusus was 67.94% extract by pressure heating extraction at $110^{\circ}C$. The content of total polyphenol compounds was the highest by 72.71 mg/g in $80^{\circ}C$ extract by reflux extraction. The highest content of total flavonoid compounds was 7.60 mg/g at $110^{\circ}C$ extract. The results of soluble protein and reducing sugar contents showed the highest content of 4.93 mg/g and 46.77 mg/g in $110^{\circ}C$ extract, respectively. In the analysis of DPPH radical scavenging activity, all extracts showed an excellent effect of 92% in 1.0 mg/mL concentration. The highest effect of ABTS radical scavenging activity was 88.67% at $110^{\circ}C$ extraction in 1.0 mg/mL concentration. Nitrite scavenging ability was the highest as 66.16% at $110^{\circ}C$ extract at pH 1.2 and 1.0 mg/mL concentration. The results of SOD-like activity and xanthine oxidase inhibition activities showed the highest effect of 14.95% and 59.45% at $110^{\circ}C$ extract in 1.0 mg/mL, respectively. Tyrosinase inhibition activity for whitening effect was the highest of 35.31% at $85^{\circ}C$ in 1.0 mg/mL. In the analysis of anti-aging effect, collagenase and elastase inhibition activity showed the highest effects of 37.78% at $110^{\circ}C$ and 20.39% at $80^{\circ}C$, respectively. All results of antioxidant activities and anti-aging effects increased along with the concentration increases. These results indicated that the extracts extracted from the flesh of C. retusus at $80^{\circ}C$ and $110^{\circ}C$ have a large amount of useful ingredients, an excellent antioxidant activity, like as DPPH and ABTS radical scavenging activity, and anti-aging effects to develop functional products than those of $25^{\circ}C$.

민간에서 식용 및 약용으로 사용되고 있으나 최근에는 활용도가 낮은 이팝나무 성숙열매 과육의 산업적 활용 증진을 위하여 상이한 추출조건(실온 $25^{\circ}C$, $80^{\circ}C$, $110^{\circ}C$)에서 물을 용매로 추출된 이팝나무 과육 3가지 추출물에 대한 항산화 및 항노화 활성을 측정하였다. 총 폴리페놀 화합물은 환류추출기를 이용한 80W에서 72.71 mg/g으로 가장 많이 함유하였으며, 플라보노이드 화합물은 가압열수 추출기를 이용 110W 추출물에서 7.608 mg/g을 함유하였다. 수용성 단백질과 환원당의 함량도 110W에서 각각 4.93 mg/g과 46.77 mg/g으로 가장 많이 함유하였다. DPPH radical 소거율은 1.0 mg/mL에서 모든 추출물이 92% 이상의 소거율을 보였으며, ABTS radical 소거활성은 110W가 88.67%였으며, 80W(87.49%)와 유의적 차이는 없었다. 아질산염 소거능은 pH 1.2의 조건에서 110W가 66.16%였으며, pH 3.0에서는 80W에서 37.86%를 나타내었다. SOD 유사활성 및 xanthine oxidase 저해활성에서도 추출온도가 가장 높은 110W에서 각각 14.95%와 59.45%의 활성을 나타내었다. 추출온도에 따른 이팝나무 과육의 미백효과를 알아보고자 tyrosinase 저해율을 측정한 결과, 환류추출기를 이용한 80W 추출물이 35.31%로 비교적 높은 저해율을 나타내었다. Collagenase에 대한 저해효과는 1.0 mg/mL에서 110W가 37.78%로 가장 우수하였으며, elastase 저해활성은 80W 20.39%, 110W 20.33%로 유사한 저해효과를 나타내었다. 모든 실험에서 농도 의존적으로 항산화 및 항노화 활성이 증가하는 경향을 보였으며, 실온보다는 높은 온도에서 추출한 이팝나무 과육 추출물에서 유용성분의 함량과 생리활성이 높게 나타났다. 이는 추출온도가 높을수록 유용성분의 추출이 용이해져 다양한 항산화 및 항노화 활성을 나타내는 것으로 사료되며, 이팝나무 과육은 기능성 식품 및 제품을 위한 천연소재로 활용 가능할 것으로 판단된다.

Keywords

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