Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Improvement of colitis preventive effects of Gochujang by addition of Lactobacillus plantarum on C57BL/6 mice

Lactobacillus plantarum 첨가 고추장의 C57BL/6 마우스에서 대장염 예방 증진효과

  • Park, Eui-Seong (Department of Food and Nutrition, College of Human Ecology, Yonsei University) ;
  • Heo, Ju-Hee (Microbial Institute for Fermentation Industry) ;
  • Lim, Yaung-Iee (Department of Food and Nutrition, Sungshin Women's University) ;
  • Ju, Jaehyun (Department of Food Science and Biotechnology, Cha University) ;
  • Park, Kun-Young (Department of Food Science and Biotechnology, Cha University)
  • 박의성 (연세대학교 식품영양학과) ;
  • 허주희 (발효미생물산업진흥원) ;
  • 임양이 (성신여자대학교 식품영양학과) ;
  • 주재현 (차의과학대학교 식품생명공학과) ;
  • 박건영 (차의과학대학교 식품생명공학과)
  • Received : 2017.11.20
  • Accepted : 2017.12.08
  • Published : 2017.12.30
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Abstract

Gochujang, a traditional Korean food, is fermented by mixing red pepper powder, various grain, meju and salt. Changes in the kind of ingredients and fermentation method could increase health functionalities. In this study, in vivo anti-colitis effects of gochujang prepared with mixed grains, bamboo salt baked 3 times and meju starters on DSS-induced colitis in C57BL/6 mice were studied. We prepared gochujang prepared with mixed grains (MG), bamboo salt, and Aspergillus oryzae (A) and Baccillus subtilis (B) mixed starters (MG-AB) and gochujang prepared with MG, bamboo salt and A, B and Lactobacillus plantarum (L) mixed starters (MG-ABL). MG-AB and MG-ABL significantly increased body weight and colon length compared to the control (p<0.05). MG-ABL showed significantly decreased interleukin-6 (IL-6) expression in serum compared to the control and MG-AB group (p<0.05). MG-ABL also regulated mRNA and protein levels of pro-apoptotic Bcl-2-associated X protein (Bax) and anti-apoptotic B-cell lymphoma-2 (Bcl-2) in the mice colon tissue (p<0.05). Therefore, MG-ABL exhibited the increased anticolitis effects by inhibiting damage of colon tissue, probably by regulating a pro-inflammatory cytokine of IL-6 and regulated apoptosis related genes. These results indicated that gochujang changed with good ingredients and starters had colitis preventive effects and might be due to active compounds in mixed grain and bamboo salt, and produced by L during the fermentation of gochujang.

DSS로 대장염을 유도한 C57BL/6 마우스에게 잡곡(보리, 수수, 기장)과 죽염, 종균(AB, ABL)을 접종한 메주로 만든 고추장을 섭취시켰다. MG-AB와 MG-ABL을 섭취한 마우스는 control에 비교해 대장의 길이를 증가시키고, 무게/길이비율은 감소시켜 대장염의 염증반응을 억제시켰다. MG-ABL은 혈청에서 IL-6의 발현을 control과 MG-AB군에 비해 유의적으로 감소시켰고, apoptosis 관련 인자인 Bax와 Bcl-2의 발현을 조절하여 apoptosis를 유도하였다. 따라서 MG-ABL은 발효 시 첨가된 Lab. plantarum로 인해 대장조직의 손상을 억제하고 전염증성 사이토카인과 apoptosis 관련 인자를 조절하여 대장염을 억제하는 것으로 나타났다.

Keywords

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