Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Changes in Serum Lipid Components and Blood Glucose by Krill (Euphausia superba) Meal and NaF in Rats

크릴 분말 및 NaF 첨가 식이가 흰쥐의 혈청 지질성분 및 혈당 변동에 미치는 영향

  • Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University) ;
  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Young-Geun (Department of Food Science and Technology, Pusan National University) ;
  • Kang, Dong-Soo (Department of Marine Bio Food Science, Chonnam National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • 진동혁 (부산대학교 식품공학과) ;
  • 오다영 (부산대학교 식품공학과) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 강동수 (전남대학교 해양바이오식품학과) ;
  • 김한수 (부산대학교 식품공학과)
  • Received : 2017.11.04
  • Accepted : 2017.12.04
  • Published : 2017.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to examine the changes in serum lipid components and blood glucose by krill (Euphausia superba) meal and sodium fluoride (NaF) in rats fed on experimental diets and administered orally to NaF 10 mg for 5 weeks. Body weight of rats decreased as the amount of krill meal diet increased, it was observed the basal diet plus NaF group (BF group) compared to the lower basal diet group (BD group). The serum concentrations of total cholesterol, LDL (low-density lipoprotein) cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL) and blood glucose in serum were higher in the BF group than the 10% krill meal group plus NaF 10 mg (KF10 group) or BD group, the 20% krill meal plus NaF 10 mg group (KF20 group), the 30% krill meal plus NaF 10 mg group (KF30 group). Conversely depending on the content of krill meal for the HDL (high-density lipoprotein) cholesterol level, it showed higher results. The concentration of total protein was no significant difference among the groups (p<0.05). The results indicate that a krill meal diet effectively inhibited increases in lipid elevation and blood glucose level in the sera of rats.

Krill (Euphausia superba)과 NaF가 Sprague Dawley계 수컷 흰쥐의 체중 및 장기 중량, 혈청 지질성분 및 단백질 함량에 미치는 영향을 조사하기 위하여 기본식이를 급여한 대조군인 BD군을 비롯하여 기본식이에 NaF 10 mg을 경구 투여한 BF군, 10%(KF10군), 20%(KF20군), 30%(KF30군)의 krill meal을 첨가 식이에 NaF 10 mg 경구 투여한 군으로 군당 6마리씩 5가지 군으로 나누어 5주간 사육한 결과는 다음과 같다. 흰쥐의 체중은 식이의 krill meal 함량이 증가함에 따라 감소하였고, 대조군인 BD군에 비해 NaF를 경구 투여한 BF군에서 낮게 관찰되었다. 각 장기무게에서의 변화는 간에서 BD군에 비해 NaF 경구 투여 군에서 유의적으로 낮게 나타났으며(p<0.05), 고환에서의 무게는 KF10군을 제외한 NaF 경구 투여 군에서 높은 것으로 관찰되었다. 심장, 폐, 뇌, 신장, 췌장에서의 각 군의 장기무게는 유의적인 차이가 없었다(p<0.05). 혈청 중 total cholesterol, LDL cholesterol, free cholesterol, triglyceride, phospholipid, 혈당 농도는 대조군인 BD군보다 BF군에서 높았지만, krill meal 함량이 증가함에 따라 감소하였고, HDL cholesterol 농도는 BD군보다 BF군에서 낮았으며 krill meal 함량에 따라 점차 증가하는 것으로 관찰되었다. 혈청 중 total protein은 각 군에서 유의적 차이(p<0.05)가 없는 것으로 나타났으며, albumin 농도와 albumin/globulin ratio는 BD군과 BF군은 비슷한 결과가 나타났으나 krill meal 섭취 군에서 감소되었다. Globulin 농도는 KF10군, KF20군, KF30군, BD군, BF군 순으로 높게 관찰되었다. 이와 같은 결과로 미루어 보아, krill meal 식이는 NaF 경구 투여한 흰쥐의 체중, 혈청 지질성분 및 혈당 조절 효과가 있는 것으로 사료된다.

Keywords

References

  1. E. J. Cho, K. J. Moon, "Related factors of medication adherence in patients with dyslipidemia: The 2010-2012 Korean National Health and Nutrition Examination Survey", Korean J. Health Edu. Promot., Vol.32, No.2 pp. 65-74, (2015). https://doi.org/10.14367/kjhep.2015.32.2.65
  2. S. M. Grundy, "Hypertriglyceridemia, atherogenic dyslipidemia, and the metabolic syndrome", American J. Cardiol., Vol.81, No.4 pp. 18B-25B, (1998). https://doi.org/10.1016/S0002-9149(98)00033-2
  3. E. K. Speliotes, J. M. Massaro, U. Hoffmann, R. S. Vasan, J. B. Meigs, D. V. Sahani, J. N. Hirschhorn, C. S. Fox, "Fatty liver is associated dyslipidemia and dysglycemia independent of visceral fat: the Framingham Heart Study", Hepatology, Vol.51, No.6 pp. 1979-1978, (2010). https://doi.org/10.1002/hep.23593
  4. G. Assmann, H. Schulte, P. Cullen, "New and classical risk factors-the Munster heart study (PROCAM)", European J. Med. Res., Vol.2, No.6 pp. 237-242, (1997).
  5. K. Izumi-Nagai, N. Nagai, K. Ohgami, S. Satofuka, Y. Ozawa, K. Tsubota, S. Ohno, Y. Olike, S. Ishida, "Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin", Invest. Ophthalmol, Visual Sci., Vol.49, No.4 pp. 79-1685, (2008). https://doi.org/10.1167/iovs.07-1426
  6. R. A. DeFronzo, E. Ferrannini, "Insulin resistance: A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease", Diabetes Care, Vol.14, No.3 pp. 173-194, (1991). https://doi.org/10.2337/diacare.14.3.173
  7. T. Xu, J. T. Zhang, M. Yang, H. Zhang, W. Q. Liu, Y. Kong, T. Xu, Y. H. Zhang, "Dyslipidemia and outcome in patients with acute ischemic stroke", Biomed. Environ. Sci., Vol.27, No.2 pp. 106-110, (2014). https://doi.org/10.3967/bes2014.023
  8. H. L. Suh, S. A. Kim, H. Y. Soh, "Distribution and abundance of the antarctic Krill (Euphausia superba) : A brief overview", Korean J. Polar Res., Vol.2, No.2 pp. 29-49, (1991).
  9. H. S. Kim, M. A. Kim, Y. Duan, S. H. Jang, D. S. Kang, W. K. Lee, C. S. Lee, J. Y. Ryu, "Fatty acid compositions, mineral and vitamin contents of the antarctic Krill (Euphausia superba)", J. Environ. Sci. Int., Vol.23, No.1 pp. 47-52, (2014). https://doi.org/10.5322/JESI.2014.23.1.47
  10. H. S. Kim, M. A. Kim, Y. Duan, D. S. Kang, S. H. Jang, J. Y. Ryu, C. S. Lee, W. K. Lee, "Studies on the nutritional components and amino acid compositions of Krill (Euphausia superba)", J. Environ. Sci. Int., Vol.23, No.2 pp. 165-170, (2014). https://doi.org/10.5322/JESI.2014.23.2.165
  11. J. L. Grandois, E. Marchioni, M. Zhao, F. Giuffrida, S. Ennahar, F. Bindler, "Investigation of natural phosphatidylcholine sources: Separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species", J. Agric. Food Chem., Vol.57, No.14 pp. 6014-6020, (2009). https://doi.org/10.1021/jf900903e
  12. M. Muriuki, N. Purdie, G. Dumancas, "Quantification of the major ${\omega}-3$, ${\omega}-6$ PUFAs using the purdie assay and their ratios in different cholesterol types and the effects of gender and cholesterol on PUFA levels", Trends Analyt. Chem., Vol.31, pp. 157-164, (2012). https://doi.org/10.1016/j.trac.2011.10.005
  13. J. C. Gigliotti, M. P. Davenport, S. K. Beamer, J. C. Tou, J. Jaczynski, "Extraction and characterisation of lipids from antarctic krill (Euphausia superba)", Food Chem., Vol.125, No.3 pp. 1028-1036, (2011). https://doi.org/10.1016/j.foodchem.2010.10.013
  14. S. K. Rhee, D. S. Kim, "The effective utilization techniques of Krill resources in antarctic ocean as new protein food", J. Korean Professional Engineers Association, Vol.32, No.1 pp. 90-98, (1999).
  15. F. S. H. Lu, B. O. Bruheim, C. Jacobsen, "Effect of temperature towards lipid oxidation and non-enzymatic browning reactions in krill oil upon storage", Food Chem., Vol.157, pp. 398-407, (2014). https://doi.org/10.1016/j.foodchem.2014.02.059
  16. J. Sun, X. Mao, "An environmental friendly process for Antarctic krill (Euphausia superba) utilization using fermentation technology", J. Cleaner Production, Vol.127, pp. 618-623, (2016). https://doi.org/10.1016/j.jclepro.2016.04.020
  17. L. Z. Wang, C. H. Xue, Y. Xue, Y. M. Wang, Z. J. Li, "Optimization and evaluation of a novel technique for hydrolyzing Antarctic krill (Euphausia superba) proteins", Food Bioproducts Processing, Vol.94, pp. 629-636, (2015). https://doi.org/10.1016/j.fbp.2014.08.010
  18. H. S. Kim, M. A. Kim, S. H Jang, "Influences of Korean haw (Crataegus pinnatifida Bunge) on lipid concentration in hypercholesterolemia", J. Environ. Sci. Int., Vol.23, No.5 pp. 793-800, (2014). https://doi.org/10.5322/JESI.2014.5.793
  19. H. S. Kim, M. A. Kim, S. H. Jang, "Improvement effect of hyperlipidemia by wild haw (Crataegus pinnatifida Bunge)", J. Environ. Sci. Int., Vol.23, No.5 pp. 787-792, (2014). https://doi.org/10.5322/JESI.2014.5.787
  20. M. F. Lee, C. S. Lai, A. C. Cheng, J. S. Hou, V. Badmaev, C. T. Ho, M. H. Pan, "Krill oil and xanthigen separately inhibit high fat diet induced obesity and hepatic triacylglycerol accumulation in mice", J. Functional Foods, Vol.19, pp. 913-921, (2015). https://doi.org/10.1016/j.jff.2014.10.015
  21. D. Shanthakumari, S. Srinivasalu, S. Subramanian, "Effect of fluoride intoxication on lipidperoxidation and antioxidant status in experimental rats", Toxicology, Vol.204, No.2 pp. 219-228, (2004). https://doi.org/10.1016/j.tox.2004.06.058
  22. H. S. Kim, J. H. Seong, Y. G. Lee, C. L. Xie, W. S. Choi, S. H. Kim, H. D. Yoon, "Effect of low-molecular-weight collagen peptide extract isolated from scales of the Flathead Mullet (Mugil cephalus) on lipid metabolism in hyperlipidemic rats", Korean J. Food Preserv., Vol.16, No.6 pp. 938-945, (2009).
  23. H. S. Kim, H. D. Yoon, "Effects of the chitosan oligosaccharide intake on the improvement of serum lipid level in hypercholesterolemic rats", J. Life Sci., Vol.18, No.12 pp. 1686-1692, (2008). https://doi.org/10.5352/JLS.2008.18.12.1686
  24. K. H. Lee, S. Y. Yoon, H. K. Kim, "Effect of crab shell powder on lipid metabolism in diet-induced hyperlipidemic rats", J. Korean Soc. Food Sci. Nutr., Vol.29, No.3 pp. 453-459, (2000).
  25. M. Y. Shon, M. H. Chung, S. K. Park, Y. S. Cho, "Effects of krill and cadmium on lipid composition of plasma in cholesterolfed rats", J. Korean Soc. Food Sci. Nutr., Vol.23, No.1 pp. 38-43, (1994).
  26. H. S. Kim, "Effects of the Saururus chinensis Baill hot-water extract intake on the lipid components and metabolic enzyme activities in hyperlipidemic rats", Korean J. Exercise Nutrition, Vol.10, No.2 pp. 99-106, (2006).
  27. H. S. Kim, H. D. Yoon, "Effects of the chitosan oligosaccharide intake on the improvement of serum lipid level in hypercholesterolemic rats", J. Life Sci., Vol.18, No.12 pp. 1686-1692, (2008). https://doi.org/10.5352/JLS.2008.18.12.1686
  28. G. D. J. B. Sudlow, D. J. Birkett, D. N. Wade, "Further characterization of specific drug binding sites on human serum albumin", Molecular pharmacology, Vol.12, No.6 pp. 1052-1061, (1976).
  29. A. M. Dalin, U. Magnusson, J. Haggendal, L. Nyberg, "The effect of transport stress on plasma levels of catecholamines, cortisol, corticosteroid-binding globulin, blood cell count, and lymphocyte proliferation in pigs", Acta veterinaria scandinavica, Vol.34, No.1 pp. 59-68, (1992).
  30. K. A. Kim, "Understanding and application of liver function tests", Korean J. Med., Vol.76, No.2 pp. 163-168, (2009).
  31. A. O. Duran, M. Inanc, H. Karaca, I. Dogan, V. Berk, O. Bozkurt, E. Ozaslan, M. Ucar, C Eroglu, M. Ozkan, "Albuminglobulin ratio for prediction of long-term mortality in lung adenocarcinoma patients", Asian Pacific J. cancer prevention: APJCP., Vol.15, No.15 pp. 6449-6453, (2013). https://doi.org/10.7314/APJCP.2014.15.15.6449
  32. A. C. Kibrick, A. B. Clements, "A comparative study of the serum albumin-globulin ratio, the cephalin-cholesterol flocculation, and the thymol turbidity tests for liver function", J. Lab. Clinic. Med., Vol.33, No.6 pp. 662-671, (1948).