Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation, Identification, and Expression of Microbial Cellulases from the Gut of Odontotermes formosanus

  • Duan, Jiwei (Key Laboratory of Microbiology, School of Life Science, Heilongjiang University) ;
  • Liu, Jun (College of Life Science Engineering, Qiqihar University) ;
  • Ma, Xueling (Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University) ;
  • Zhang, Yue (Key Laboratory of Microbiology, School of Life Science, Heilongjiang University) ;
  • Wang, Xiaohua (Key Laboratory of Microbiology, School of Life Science, Heilongjiang University) ;
  • Zhao, Kai (Key Laboratory of Microbiology, School of Life Science, Heilongjiang University)
  • Received : 2016.04.14
  • Accepted : 2016.09.28
  • Published : 2017.01.28
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Abstract

Termites are destructive to agriculture, forestry, and buildings, but they can also promote agro-ecosystem balance through the degradation of lignocellulose. Termite-triggered cellulose digestion may be clarified through microbial metabolism of cellulose products. In the present study, we characterized the activities of cellulase and its three components synthesized by the cellulase-producing fungal strain HDZK-BYTF620 isolated from the gut of Odontotermes formosanus. The protein components of cellulases were synthesized by strain HDZK-BYTF620, which were isolated and characterized using polyacrylamide gel electrophoresis, and the expression of the cellulases was studied at the proteome level.

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