Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant activities of phenolic compounds from Angelica gigas Nakai extract using subcritical water

아임계수 추출 기술을 이용한 당귀 추출물의 유효성분 및 산화방지 평가

  • Ko, Min-Jung (Department of Food Science and Engineering, Ewha Womans University) ;
  • Lee, Jeong-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Nam, Hwa-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University)
  • 고민정 (이화여자대학교 식품공학과) ;
  • 이정현 (이화여자대학교 식품공학과) ;
  • 남화현 (이화여자대학교 식품공학과) ;
  • 정명수 (이화여자대학교 식품공학과)
  • Received : 2016.07.08
  • Accepted : 2016.09.02
  • Published : 2017.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Subcritical water extraction can be used to selectively extraction compounds by varying the temperature-dependent dielectric constant of water. This study investigated subcritical water extraction of decursin and nodakenin yields from Angelica gigas Nakai (AN) quantitatively and qualitatively by HPLC, and HPLC-ESI/MS. Total phenolics, total flavonoid contents, and antioxidant activity were determined by DPPH and ABTS radical scavenging activity, including the effects of varying the extraction conditions of temperature ($110-200^{\circ}C$) and time (1-20 min) under high pressure (10 MPa). By subcritical water extraction under operating conditions of $120-130^{\circ}C$, the maximum yields of decursin ($6.64{\pm}0.42%$ in the dried material) and nodakenin ($3.71{\pm}0.28%$ in the dried material) were obtained. From $190-200^{\circ}C$ the maximum yields of total phenolics ($75.97{\pm}1.64mg$ gallic acid equivalent/g AN), flavonoids ($8.56{\pm}1.10mg$ quercetin equivalent/g AN), DPPH ($63.07{\pm}1.71%$), and ABTS ($72.32{\pm}2.82%$) were obtained.

아임계수 추출 기술을 이용하여 참당귀로부터 데쿠신, 노다케닌을 추출하고 HPLC, HPLC-ESI/MS를 이용하여 추출조건에 따른 유효성분을 정성 및 정량적으로 비교 분석하였다. 추출결과, 데쿠신과 노다케닌의 추출함량은 아임계수 추출법과 비교하여 열수 추출 방법을 이용하는 것이 약간 많거나 유사하였지만, 추출 시간은 아임계수 추출법이 5-10분 이내로 매우 빠르게 추출할 수 있는 장점이 있다. 그리고 총 페놀함량, 총 플라보노이드 함량은 아임계수 추출법이 우수하게 많이 추출됨을 확인 할 수 있었고, DPPH, ABTS 라디칼 소거능을 이용한 산화방지 효과도 아임계수 추출법이 우수하였다. 특히, 아임계수 추출 조건인 추출 온도와 추출 시간이 증가할수록 총 페놀함량 및 총 플라보노이드 함량, 그리고 산화방지 효과 또한 점차적으로 증가하는 경향을 나타내었고 높은 상관관계를 보였다. 이는 오직 물만을 사용하여 유효성분을 추출하는 친환경적이며 안전하고 독성이 없는 아임계수 추출의 잠재성과 우수성을 확인한 결과이며, 다양한 한약재뿐만 아니라 다른 식품 추출 산업에 응용하여 사용될 수 있을 것이다.

Keywords

References

  1. Kang SA, Han JA, Jang KH, Choue RW. DPPH radical scavenger activity and antioxidant effects of chan-dang-gui (Angelica gigas). J. Korean Soc. Food Sci. Nutr. 33: 1112-1118 (2004) https://doi.org/10.3746/jkfn.2004.33.7.1112
  2. Um YR, Lee JH, Ma JY. Quantitative analysis of marker substances in solid fermented Angelicae gigantis radix by HPLC. Korean J. Oriental Med. 16: 173-178 (2010)
  3. Park KW, Choi SR, Shon MY, Jeong IY, Kang KS, Lee ST, Shim KH, Seo KI. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J Korean Soc. Food Sci. Nutr. 36: 1385-1390 (2007) https://doi.org/10.3746/jkfn.2007.36.11.1385
  4. Cha, JY, Kim HW, Heo JS, Ahn HY, Eom KE, Heo SJ, Cho YS. Ingredients analysis and biological activity of fermented Angelica gigas Nakai by mold. J. Life Sci. 20: 1385-1393 (2010) https://doi.org/10.5352/JLS.2010.20.9.1385
  5. Heo JS, Cha JY, Kim HW, Ahn HY, Eom KE, Heo SJ, Cho YS. Bioactive materials and biological activity in the extracts of leaf, stem mixture and root from Angelica gigas Nakai. J. Life Sci. 20: 750-759 (2010) https://doi.org/10.5352/JLS.2010.20.5.750
  6. Hwang JB, Yang MO. Comparison of chemical components of Angelica gigas Nakai and Angelica acutiloba Kitagawa. Korean J. Food Sci. Technol. 29: 1113-1118 (1997)
  7. Ko MJ, Cheigh CI, Cho SW, Chung MS. Subcritical water extraction of flavonol quercetin from onion skin. J. Food Eng. 102: 327-333 (2011) https://doi.org/10.1016/j.jfoodeng.2010.09.008
  8. Ko MJ, Cheigh CI, Chung MS. Relationship analysis between flavonoids structure and subcritical water extraction (SWE). Food Chem. 143: 147-155 (2014) https://doi.org/10.1016/j.foodchem.2013.07.104
  9. Ko MJ, Cheigh CI, Chung MS. Optimization of subcritical water extraction of flavanols from green tea leaves. J. Agr. Food Chem. 65: 6828-6833 (2014)
  10. Ko MJ, Kwon HL, Chung MS. Optimum conditions for extracting flavanones from grapefruit peels and encapsulation of extracts. Korean J. Food Sci. Technol. 46: 465-469 (2014) https://doi.org/10.9721/KJFST.2014.46.4.465
  11. Cicco N, Lanorte MT, Paraggio M, Viggiano M, Lattanzio V. A reproducible, rapid and inexpensive Folin-Ciocalteu micromethod in determining phenolics of plant methanol extracts. Microchem. J. 91: 107-110 (2009) https://doi.org/10.1016/j.microc.2008.08.011
  12. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  13. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  14. Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic cotribution to total antioxidant activity. Food Chem. 73: 239-244 (2001) https://doi.org/10.1016/S0308-8146(00)00324-1
  15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  16. Ahn MJ, Lee MK, Kim YC, Sung SH. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J. Pharmaceut. Biomed. 46: 258-266 (2008) https://doi.org/10.1016/j.jpba.2007.09.020
  17. Park JH, Jung JW, Kweon KT, Seo MJ, Seo EK, Park YK, Lee JH. Nodakenin and decursin contents of fermented Angelicae gigantis radix by 4 species strain. Korean J. Herbol. 25: 7-10 (2010)
  18. Li HB, Wong CC, Cheong KW, Chen F. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Sci. Technol. 41: 385-390 (2008) https://doi.org/10.1016/j.lwt.2007.03.011
  19. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Food Drug Anal. 22: 296-302 (2014) https://doi.org/10.1016/j.jfda.2013.11.001
  20. Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem. 112: 303-309 (2009) https://doi.org/10.1016/j.foodchem.2008.05.077
  21. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agr. Food Chem. 51: 2144-2155 (2003) https://doi.org/10.1021/jf020444+