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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Study of Anti-microbial Activities and Anti-inflammatory Effects of Chamomile (Matricaria chamomilla) Extracts in HaCaT cells

HaCaT 세포주에서 캐모마일 (Matricaria chamomilla) 추출물의 항병원 성 및 항염 효과에 관한 연구

  • Lim, Eun Gyeong (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Guen Tae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Bo Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Eun Ji (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Sang-Yong (Department of Food Science & Bio Technology, Shinansan University) ;
  • Han, Nam Kyu (Dr. HASKIN Co., Ltd.) ;
  • Ha, Jae Sun (Dr. HASKIN Co., Ltd.) ;
  • Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
  • 임은경 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김근태 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김보민 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김은지 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김상용 (신안산대학교 식품생명과학과) ;
  • 한남규 (닥터하스킨 주식회사) ;
  • 하재순 (닥터하스킨 주식회사) ;
  • 김영민 (한남대학교 생명나노과학대학 생명시스템과학과)
  • Received : 2017.01.02
  • Accepted : 2017.02.02
  • Published : 2017.03.31
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Abstract

Chamomile (Matricaria chamomilla), a member of the Asteraceae family, is a well-known for medicinal plant and can be found in India and Europe. Chamomile is an effective sedative and various medical effects. But, the effects of acne treatment by chamomile were not investigated. Therefore, we assessed the anti-oxidant effects, anti-microbial activity and anti-inflammatory effects by chamomile extracts in HaCaT keratinocyte cells. Anti-oxidant effects of chamomile extracts were investigated by DPPH assay. Also, results of MTT assay was demonstrated that chamomile extracts did not have a cytotoxic effect in HaCaT cells. To assess the antimicrobial activity, we determined formation of inhibition zone of Propionibacterium acnes by extracts from chamomile. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) induces production of inflammatory cytokines such as interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6 and IL-8 and expression of COX-2. Chamomile extracts could inhibit TNF-${\alpha}$-induced mRNA expression levels of IL-$1{\beta}$, IL-6, IL-8 and COX-2 gene. These results demonstrated the possibility of chamomile for prevention and treatment of skin inflammatory diseases such as acne.

Keywords

References

  1. Lee, W. G., B. H. Kim, S. W. Jeon, K. S. Kim, H. J. Nam, and Y. B. Kim (2011) The Latest Trends of Treatment for Acne Vulgaris in PubMed. J. Korean Orient. Med. Ophthalmol. Otolaryngol. Dermatol. 24: 41-56.
  2. Jin, B. W., J. H. Ahn, and C. J. Ma (2011) Antibacterial Activity of Some Medicinal Plants against Propionibacterium acnes. Kor. J. Pharmacogn. 42: 98-101.
  3. Yoon, H. J. and D. I. Kim (2015) Review on the Acne Related Articles Published in Korean Medical Journals-Focusing on Experimental Studies Published after 2005. J. Korean Obstet. Gynecol. 28: 113-127. https://doi.org/10.15204/jkobgy.2015.28.1.113
  4. An, B. J., J. T. Lee, C. E. Lee, J. H. Son, J. Y. Lee, and T. S. Park (2005) A Study on the Development of Cosmeceutical Ingredient, Rhododendron mucronulatum, and the Application of Rheology Properties. J. Korean Soc. Appl. Biol. Chem. 48: 273-279.
  5. Eom, J. N. and J. D. Kim (2004) An empirical study on the oriental herbal cosmetics purchase behaviors in women in the Metropolitan area. J. Soc. Cosmet. Scientists Korea. 30: 93-102.
  6. Cho, Y. J., S. J. Yoon, J. H. Kim, and S. S. Chun (2005) Biological Activity of Chamomile (Matricaria chamomilla L.) Extracts. J. Korean Soc. Food Sci. Nutr. 34: 446-450. https://doi.org/10.3746/jkfn.2005.34.4.446
  7. Lee, S. H., K. J. Min, K. O. Lee, J. S. Sin, Y. C. Kim (2008) Effect of German Chamomile Oil Application to Atopic Dermatitis Mice on the Change of Serum IgE Level. J. Kor. Soc. Cosm. 14: 337-345.
  8. Srivastava, J. K., E. Shankar, and S. Gupta (2011) Chamomile: A herbal medicine of the past with bright future. Mol. Med. Report. 3: 895-901.
  9. Kim, J. H., S. Y. Kim, Y. N. Hong, Y. S. Kim, and Y. M. Han (2014) Effect of Chamomile Flower Extract on Septic Arthritis due to Candida albicans. Yakhak Hoeji. 58: 343-348.
  10. Abebe, W. (2002) Herbal medication: potential for adverse interactions with analgesic drugs. J. Clin. Pharm. Ther. 27: 391-401. https://doi.org/10.1046/j.1365-2710.2002.00444.x
  11. Weidner, C., S. J. Wowro, M. Rousseau, A. Freiwald, V. Kodelja, H. Abdel-Aziz, O. Kelber, and S. Sauer (2013) Antidiabetic effects of chamomile flowers extract in obese mice through transcriptional stimulation of nutrient sensors of the peroxisome proliferator-activated receptor (PPAR) family. PLoS One. 8: e80335. https://doi.org/10.1371/journal.pone.0080335
  12. Ha, J. H., Y. J. Jeong, J. S. Seong, K. M. Kim, A. Y. Kim, M. M. Fu, J. Y. Suh, N. H. Lee, J. Park, and S. N. Park (2015) Antioxidant and Antibacterial Activities of Glycyrrhiza uralensis Fisher (Jecheon, Korea) Extracts Obtained by various Extract Conditions. J. Soc. Cosmet. Sci. Korea. 41: 361-373.
  13. Choi, I. Y., Y. J. Song, and W. H. Lee (2010) DPPH radical scavenging effect and antimicrobial activities of some herbal extracts. Kor. J. Hort. Sci. Technol. 28: 871-87.
  14. Beissert, S., I. Cavazzana, F. Mascia, P. Meroni, S. Pastore, G. Tessari, and G. Girolomoni (2006) Mechanisms of immune-mediated skin diseases: An overview. Clin. Exp. Rheumatol. 24: S1-6.
  15. Lee, W. R., K. H. Kim, H. J. An, J. Y. Kim, S. M. Han, K. G. Lee, and K. K. Park (2014) The effects of bee venom on tumor necrosis factor (TNF)-${\alpha}$ induced inflammatory human HaCaT keratinocytes. Kor. J. Pharmacogn. 45: 256-261.
  16. Sohn, H. Y., Y. S. Kim, E. J. Kum, Y. S. Kwon, and K. H. Son (2006) Screening of anti-acne activity of natural products against Propionibacterium acnes. Kor. J. Microbiol. Biotechnol. 34: 265-272.
  17. Zu, Y. G., H. M. Yu, L. Liang, Y. J. Fu, T. Efferth, X. Liu, and N. Wu (2010) Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules. 15: 3200-3210. https://doi.org/10.3390/molecules15053200
  18. Singh, O., Z. Khanam, N. Misra, and M. K. Srivastava (2011) Chamomile (Matricaria chamomilla L.): An overview. Pharmacogn Rev. 5: 82-95. https://doi.org/10.4103/0973-7847.79103
  19. Taylor, M., M. Gonzalez, and R. Porter (2011) Pathways to inflammation: acne pathophysiology. Eur. J. Dermatol. 21: 323-333.
  20. Choi, J. Y., S. Y. Song, and H. H. Lee (2015) Antibacterial and antiinflammatory activity of corni fructus ethanol extract in Propionibacterium acnes. Kor. J. Aesthet. Cosmetol. 13: 623-630.
  21. Lee, H. J. and Y. C. Chang (2012) Suppression of TNF-${\alpha}$-induced inflammation by extract from different parts of moringa in HaCaT cells. J. Life Sci. 22: 1254-1260. https://doi.org/10.5352/JLS.2012.22.9.1254
  22. Bordon, Y. (2010) Cytokines: Cytokines reach out. Nat. Rev. Immunol. 10: 382-383 . https://doi.org/10.1038/nri2791

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