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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Measurement Uncertainty for Analytical Method of Hyaluronic Acid Used as a Dietary Supplement

식이보충제로 사용되는 히알루론산의 분석법에 대한 불확도 산정

  • Park, Sang-Wook (Department of Food and Drug, Chosun University Graduate School) ;
  • Kim, Hyung Bum (Department of Food and Drug, Chosun University Graduate School) ;
  • Kim, Kwang Joon (Department of Pharmacy, Chosun University Hospital) ;
  • Lee, Wonjae (Department of Food and Drug, Chosun University Graduate School)
  • 박상욱 (조선대학교 대학원 식품의약학과) ;
  • 김형범 (조선대학교 대학원 식품의약학과) ;
  • 김광준 (조선대학교병원 약제부) ;
  • 이원재 (조선대학교 대학원 식품의약학과)
  • Received : 2016.10.22
  • Accepted : 2017.03.23
  • Published : 2017.03.31
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Abstract

This study aimed to estimate the measurement uncertainty related to determination of hyaluronic acid used as a dietary supplement by high-performance liquid chromatography. According to the guidelines of the Association of Official Analytical Chemists, the analytical processes for determination of hyaluronic acid were performed. And the measurement uncertainty obtained during the analytical processes were expressed in accordance with mathematical/ statistical guidances of GUM (Guide to the Expression of Uncertainty in Measurement) & EURACHEM (Focus for Analytical Chemistry in Europe) for the analytical operations. For the uncertainty in measurement produced based on this analytical method, the expanded uncertainty was calculated by using the relative standard uncertainty between analytical results and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc). In the results of 95% confidence interval, it was calculated that the uncertainty in measurement was $57.75{\pm}8.76{\mu}g/kg$ (k=2.0). Therefore, it showed that the measurement uncertainty obtained by this analytical method influences on 15.2% of the contents of hyaluronic acid as the analytical results.

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References

  1. Meyer, K. and J. W. Palmer (1934) The polysaccharide of the vitreous humor. J. Biol. Chem. 107: 629-634.
  2. Scott, J. E. (1989) Secondary structures in hyaluronan solutions: chemical and biological implications. Ciba. Found. Symp. 143: 6-15.
  3. Laurent, T. C. (1987) Biochemistry of hyaluronan. Acta. Otolaryngol. Suppl. 442: 7-24.
  4. Necas, J., L. Bartosikova, P. Brauner, and J. Kolar (2008) Hyaluronic acid (hyaluronan): a review, Vet. Med. 53: 397-411. https://doi.org/10.17221/1930-VETMED
  5. Ministry of Food and Drug Safety (MFDS), Information for health functional food. http://www.foodnara.go.kr/hfoodi/. (2016).
  6. Ministry of Food and Drug Safety (MFSD), Notice #2014-204, Chungcheongbuk-do, Republic of Korea. http://www.mfds. go.kr/index.do?mid=686&seq=8711&cmd=v. (2016).
  7. ISO/IEC, Uncertainty of measurement Part 3: Guide to the expression of uncertainty in measurement. http://www.iso.org/obp/ui/#iso:std:iso-iec:guide:98:-3:ed-1:c2:en. (2016).
  8. EURACHEM/CITAC Guide, Measurement uncertainty arising from sampling: A guide to methods and approaches, Eurachem, EUROLAB, CITAC, Nordtest and the RSC Analytical Methods Committee. http://eurachem.org/images/stories/Guides/pdf/UfS_2007.pdf. (2016).
  9. Chwae, J. H. (2009) Study on international accreditation for residue analysis and uncertainty in measurement. Korean Soc. Environ. Agr. 1: 105-115.
  10. Kim, J. Y. (2016) Assessment of regression model, linearity and weighting factor for preparation of calibration curve in the quantitative LC-MS/MS measurements of methylphenidate and its metabolite. Yakkak Hoeji. 60: 304-311. https://doi.org/10.17480/psk.2016.60.6.304
  11. Park, S. W., J. S. Bang, and W. Lee (2016) A study on the statistical method for the target component of a glucose-lowering functional material in extracts of evening primerose seeds. Korean J. Clin. Pharm. 26: 70-76.