Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Molecular epidemiological study of measles virus throughout an imported epidemic outbreak in Gyeonggi-do in 2014

2014년 경기지역에서 발생한 해외유입 홍역 바이러스의 분자역학적 특성 분석

  • Yun, Hee-Jeong (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Park, Po-Hyun (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Hwang, Sun-Il (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Huh, Jeong-Weon (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Nam, Soo-Jung (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Yong, Kum-Chan (Team of virus, Gyeonggi-do Institute of Health and Environment) ;
  • Yoon, Mi-Hye (Team of virus, Gyeonggi-do Institute of Health and Environment)
  • 윤희정 (경기도보건환경연구원 바이러스팀) ;
  • 박포현 (경기도보건환경연구원 바이러스팀) ;
  • 황선일 (경기도보건환경연구원 바이러스팀) ;
  • 허정원 (경기도보건환경연구원 바이러스팀) ;
  • 남수정 (경기도보건환경연구원 바이러스팀) ;
  • 용금찬 (경기도보건환경연구원 바이러스팀) ;
  • 윤미혜 (경기도보건환경연구원 바이러스팀)
  • Received : 2016.11.14
  • Accepted : 2017.01.26
  • Published : 2017.03.31
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Abstract

Measles virus is a highly contagious, exanthematic virus, preventable by the use of an effective live-attenuated vaccine. However, measles virus remains endemic in many area of the world causing nearly 200,000 deaths per year and still a major cause of child mortality, mostly in developing countries. In March 2014, Republic of Korea was certified as a 'national measles elimination' by the WHO as a result of a high-quality case-based surveillance system and population immunity, which was achieved by a high vaccination rate (>95.0% since 1996). But, since the beginning of 2014, the Gyeonggi province has experienced a resurgence of measles cases. In this study, we investigated the characteristics of measles viruses isolated from confirmed measles in Gyeonggi province during January 1, 2014 ~ July 31, 2014, 60 isolates were obtained from 72 confirmed measles specimens. Genotypic distributions and genetic diversities of isolated measles virus were analyzed by sequencing of nucleoprotein (N) gene. 58 (96.7%) imported cases were identified. The predominant genotype was B3, which reflects the circulating measles virus in adjacent countries. The sequences of nucleoprotein (N) gene of isolated MeV were showed that the strains characterized showed the highest degree of identity (99%) with the Philippine related strains in 2013-2014. Therefore, infected traveler returning from the Philippines transmitted secondary infection in Korea.

홍역바이러스(Measles virus)는 전염성이 매우 높은 발진성 열병을 일으키는 바이러스이며, 약독화된 생백신 접종으로 충분히 예방할 수 있다. 그러나 대부분의 개발도상국에서 어린아이들의 주요 사망원인이며 해마다 20만명이 사망하는 풍토병이다. 우리나라는 1996년 이후부터 국가적으로 시행된 정기예방접종으로 인한 높은 백신 접종률(>95.0%)로 획득된 집단면역과 고도의 기술적 감시체계의 결과로 홍역이 퇴치되었으며, 2014년 3월 WHO로부터 '홍역퇴치국가'로 인증 받았다. 그러나 2014년 초부터 경기도에서 홍역환자가 다시 발생하였다. 본 연구에서는 2014년 1월부터 2014년 7월까지 경기도에서 발생한 홍역 확진 환자를 대상으로 홍역바이러스의 유행양상 및 유전형 분포양상을 분석하였다. 72건의 홍역바이러스 양성검체에서 60주의 홍역바이러스를 분리하였고, nucleoprotein(N) 유전자의 염기서열 분석을 통하여 유전형의 확인 및 유전자변이를 분석하였다. 총 60건의 홍역 확진검체를 분석한 결과 58건(96.7%)에서 B3 genotype을 확인하였다. 또한 nucleoprotein(N) 유전자 부위의 유전자 변이 분석결과, 경기도내 분리주들이 2013년에서 2014년 필리핀에서 유행한 홍역 분리주들과 99% 일치하였다. 따라서 필리핀 등에서 감염된 후 국내에 유입되어 2차 감염이 된 것으로 사료된다.

Keywords

References

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